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Orion II Technical Data

Luminometer

Orion II Technical Data

Sample Format

96-well microplate, opaque in solid or strip format,
configuration for both 96- well and 384-well microplate available.

Detector

Photomultiplier tube with bialkali cathode, effective spectral sensitivity
range 300-600 nm, operated in photon counting mode.

Sensitivity

2 atto mole ATP.

Crosstalk

Less than 3 x 10-5.

Dynamic Range

6 decades.

Measuring Time

0.1-100 s per well.

Scan Pattern

Measurement by row or by columns.
Random selectable single well, access by mouse-click.

Injectors

Up to four per Orion II system (upgradeable).
Two injectors in pre-position, two in measurement position.

Injection Volumes

10-150 ¥ìl per pump in increments of 1¥ìl.

Injection Pattern

Double Injection Design DID: Simultaneous injection into two adjacent
wells possible in column mode as well as in row mode.

Tubing

Chemically inert PTFE tubing and connections (PTFE; KEL-F; glass; PS),
easily exchangeable liquid handling system and tips.
Minimal dead volume due to short reagent lines.

Priming

Forward and reverse priming, external priming container or built-in priming
container (optional).

Shaker

Linear, orbital and cross shaking.

Sample Incubation

Ranges for optional sample incubation:
Room temperature to 42¡ÆC, ¡¾ 1 K (bottom heating).
Room temperature to 50¡ÆC, ¡¾ 0.5 K (top and bottom heating).

Automation

Compatible to all known microplate transfer devices.

Interfaces

Serial interface (RS-232) and USB interface for PC.

Dimensions

W 385 mm, D 410 mm, H 255 mm.

Weight

22 kg.

Power

230 V 50 Hz; 115 V 60 Hz.

Power Consumption

30 VA, maximum 70 VA (4 injectors and heating).

ÀÀ¿ëºÐ¾ß
- Reporter gene assays including dual-reporter assays
- ATP assays
- Kinase activity assays
- Cell proliferation, cytotoxicity and biomass assays, apoptosis assays
- Quantification of nucleic acids
- Ca2+ measurements and Aequorin-based assays
- Luminescence immunoassays(LIA)
- Cellular luminescence
- Environmental toxicity and mutagenicity assays