Application: Protein Separation in Agarose Gels
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MetaPhor¢ç Agarose´Â polyacrylamide¸¦ ´ë½ÅÇØ¼ »ç¿ëÇÒ ¼ö ÀÖ´Â °íÇØ»óµµÀÇ agarose·Î, Áß°£ ¼öÁØÀÇ ¿ëÇØ ¿Âµµ (75¡ÆC)¸¦ ³ªÅ¸³»¸ç, ±âÁ¸ agarose¿¡ ºñÇØ 2¹è ³ôÀº ÇØ»óµµ¸¦ °¡Áö°í ÀÖ´Ù. Fast running protocolÀ» »ç¿ëÇϸé 20 cm ±æÀÌÀÇ ¼öÁ÷Çü ¶Ç´Â ¼öÆòÇü Àü±â¿µµ¿À» ÅëÇØ 1.5½Ã°£ ³»¿¡ 1% Å©±â Â÷ÀÌÀÇ DNA ´ÜÆíÀ» ±¸ºÐÇÒ ¼ö ÀÖ´Ù (¿¹¸¦ µé¾î 200 bp DNA ´ÜÆí°ú 204 bp DNA ´ÜÆíÀ» ºÐ¸®ÇÏ´Â °ÍÀÌ °¡´ÉÇÏ´Ù).
2~4% MetaPhor¢ç Agarose gelÀº 4~8% polyacrylamide gel ¼öÁØÀÇ ÇØ»óµµ¸¦ °¡Á®, AMPFLP, STR ºÐ¼® ¹× tri - tetranucleotide repeat °ËÃâ¿¡ ÀûÇÕÇϸç, ÀÛÀº Å©±âÀÇ PCR »ê¹°À» ºÐ¼®ÇÏ´Â µ¥µµ »ç¿ëÇÒ ¼ö ÀÖ´Ù.
Analytical Specifications
Gelling temperature (3%) |
¡Â35¡ÆC |
Melting temperature (3%) |
¡Â75¡ÆC |
Gel strength (3%) |
¡Ã300 g/cm©÷ |
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- All Lonza Agarose is quality tested to certify performance
- Very fine resolution of small DNA fragments
- High gel strength
- Low EEO
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Àü±â¿µµ¿ ¿¹ - 3.5% MetaPhor¢ç agarose gel

DNA ladders with 4 bp or 8 bp step sizes were prepared by ligation of Bgl II linkers. Aliquots of 0.8 ¥ìg of the ladders were separated on a 3.5% MetaPhor¢ç Agarose gel in a horizontal format and compared to an 8% polyacrylamide gel run in a vertical format in TBE buffer.
Separation range of MetaPhor¢ç Agarose
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- EtBrº¸´Ù ³ôÀº °¨µµÀÇ gel staining ½Ã¾à: GelStar¢ç Nucleic Acid Stain
- »ç¿ëÀÌ °£ÆíÇÑ premade buffer: Lonza AccuGene¢ç Buffer ½Ã¸®Áî
- Gradient PCR ±â±â: TaKaRa PCR Thermal Cycler Touch¢ç
- ³»¿¼º gel maker set¸¦ Æ÷ÇÔÇÑ µðÁöÅÐ Àü±â¿µµ¿ ÀåÄ¡: Mupid¢ç-One