PrimeScript RTase (200 U/§¡)

5 §¡

RNase Inhibitor (40 U/§¡)

5 §¡

Oligo (dT) 18 Anchor Primer (1 μg/§¡)^*1

10 §¡

5×1st Strand Synthesis Buffer^*2

20 §¡

1st Strand dNTP Mixture

6 §¡

E. coli RNase H/ E. coli DNA Ligase Mixture

10 §¡

E. coli DNA Polymerase I (20 U/§¡)

10 §¡

2nd Strand dNTP Mixture^*2

23 §¡

5×2nd Strand Synthesis Buffer^*2

150 §¡

T4 DNA Polymerase (1 U/§¡)

20 §¡

10×T4 DNA Ligase Buffer

20 §¡

T4 DNA Ligase (350 U/§¡)

20 §¡

EcoR I- Sma I Adaptor (0.4 μg/§¡)

18 §¡

Not I Supplement

135 §¡

Not I (50 U/§¡)

15 §¡

tRNA (10 §¶/§¡)

5 §¡

Dr. GenTLE Precipitation Carrier^*3

60 §¡

3 M Sodium Acetate (pH5.2)

1 §¢

pAP3neo Predigested Vector (100 ng/§¡)^*4

5 §¡

RNase-free H₂O

640 §¡

Control RNA (1 μg/§¡)^*5

5 §¡

T7 promotor primer (5 pmol/§¡)^*6

20 §¡

T3 promotor primer (for pAP3neo) (5 pmol/§¡)^*6

20 §¡

Spin Column

5 °³