The ApoAlert Glutathione Detection Kit is a quantitative in vitro assay that detects decreased cytosolic glutathione (GSH) levels that occur early in apoptosis in some cell types.

In healthy cells, glutathione acts as a redox buffer. However, in Jurkat and some other cell types, the plasma membrane contains an ATP-dependent GSH transport system that is triggered by the initiation of apoptosis. When GSH is actively pumped out of the cell, the cytosol is shifted from a reducing to an oxidizing environment.

The ApoAlert Glutathione Detection Kit includes a monochlorobimane (MCB) dye that fluoresces blue when bound to glutathione. The decrease in glutathione levels between nonapoptotic and apoptotic cell extracts is easily detected using a fluorometer or 96-well fluorescence plate reader.

Applications

Monitor early stages of apoptosis

Components

Cell Lysis Buffer
Cell Wash Buffer
Monochlorobimane (MCB)
Protocol-at-a-Glance (PT3450-2)

Storage Conditions

-20”ĘC for MCB
4”ĘC for all other components