Somatic stem cell라 불리는 adult stem cell은 다분화능을 갖고 있기 때문에 매우 중요한 치료적 가치를 지닌 미분화 세포이다. mesenchymal stem cells (hMSCs)과 adipose-derived stem cells (hADSCs), 두 종류 세포가 human adult stem cell 연구에 가장 많이 이용되고 있다. 일반적인 transfection 시약으로는 형질전환이 어려운 이 세포에 Xfect Adult Stem Cell Tranfection Reagent는 효과적으로 transfection할 수 있다.
Xfect Human Adult Stem Cell Transfection Reagent는 플라스미드 DNA와 결합하여 생분해성 나노입자를 형성하는 Xfect Transfection Reagent 계열로 낮은 세포독성과 뛰어난 트랜스펙션 효율을 갖고 있으며 hMSCs와 hADSCs에 효과적으로 트랜스펙션할 수 있도록 더 특화되어 있다.
Chondrogenic differentiation of human mesenchymal stem cells (hMSCs) is not affected by the use of Xfect Adult Stem Cell Transfection Reagent. Non-transfected hMCSs (control) and hMCSs transfected using Xfect Adult Stem Cell Transfection Reagent, were induced to differentiate by feeding with chondrogenic induction medium. The transfected cells differentiated to the same extent as the control cells, indicating that the Xfect reagent does not alter this process. Chondrogenic differentiation was confirmed by Alcian Blue staining of sulfated proteoglycans deposits.
Xfect Adult Stem Cell Transfection Reagent transfects human adipose-derived stem cells (hADSCs) with high efficiency. pAcGFP1-C1 was transfected into hADSCs using three different transfection reagents according to their respective protocols. Xfect Adult Stem Cell Transfection Reagent demonstrated a transfection efficiency that far exceeded those of the two leading competitors (L and F).
Transfection efficiency and cell viability as a function of increasing DNA. The combination of 7.5 μg of plasmid DNA with 3 μl of Xfect Polymer, achieves the optimal transfection efficiency and viability in both human mesenchymal stem cells, and human adipose-derived stem cells. Experiments performed in a 6-well plate format.