RNAiMonitor measures knockdown of RNAi target sequences efficiently and easily. A pMetLucCAV1 reporter plasmid (3 μg) containing a CAV1 target sequence, and the indicated amount of an anti-CAV1 shRNA expression vector, were cotransfected into HeLa cell cultures. A ProLabel expression vector was also included to assess transfection efficiency. At the end of the experiment, the culture medium was assayed for secreted luciferase activity, and the cells were assayed for ProLabel content. The level of luciferase knockdown correlated with the amount of cotransfected CAV1 shRNA construct.
The ProLabel Screening Kit allows you to quantitatively measure the level of any recombinant protein. The ProLabel tag and Enzyme Acceptor (EA) combine to form an active enzyme that cleaves the chemiluminescent substrate; the resulting signal can be detected with any standard luminometer.