Co-immunoprecipitation (Co-IP) is used to pull down an entire complex of intact protein complexes in order to identify unknown members of the complex.

The EpiXplore Co-Immunoprecipitation Kits are designed to evaluate protein-protein complexes, either in whole cells or in nuclear extracts. Both kits utilize specially formulated magnetic Protein G beads, which have been optimized for maximal signal-to-noise ratio. The included wash buffers enable flexibility for detection of strong or weak protein-protein interactions.

• Use the EpiXplore Co-Immunoprecipitation Kit to evaluate protein-protein complexes from whole cell extracts.
• Use the EpiXplore Nuclear Co-Immunoprecipitation Kit to evaluate protein-protein complexes from nuclear extracts. This kit includes cytoplasmic and nuclear lysis buffers to fractionate nuclear proteins. Enzymatic treatment of nuclear extracts enables release of proteins from genomic DNA.

For your convenience, Protein G is included in Clontech's Co-IP kits.

Figure 1. CoIP assay for proteins involved in epigenetic interactions. Nuclear or whole-cell extracts are incubated with a primary antibody specific for the protein of interest and magnetic beads, to isolate complexes containing the protein of interest. Protein complexes can then be analyzed further.