pAcGFP1 Vector Information

Restriction map and multiple cloning site (MCS) of pAcGFP1.

Unique restriction sites are in bold. NOTE: The Xba I and Bcl I sites are methylated in the DNA provided by Clontech Laboratories, Inc. If you wish to digest the vector with these enzymes, you will need to transform the vector into a dam- host and make fresh DNA.

Location of features

• lac promoter: 95-178
  CAP binding site: 111-124
  -35 region: 143-148; -10 region: 167-172
  Transcription start point: 179
  lac operator: 179-199
• lacZ-ECFP fusion protein expressed in E. coli
  Ribosome binding site: 206-209
  Start codon (ATG): 217-219; stop codon: 1006-1008
• 5' multiple cloning site: 234-281
  
• Aequorea coerulescens Green fluorescent protein (AcGFP1) gene
  Kozak consensus translation initiation site: 282-292
  Start codon (ATG): 289-291; stop codon: 1006-1008
  Insertion of Val at position 2: 292-294
  Last amino acid: 1003-1005
• 3' Multiple Cloning Site: 1009-1108
• Ampicillin resistance gene:
  Promoter: -35 region: 1484-1450, -10 region: 1507-1512
  Ribosome binding site: 1542-1546
  ет-lactamase coding sequences:
  start codon (ATG): 1554-1556; stop codon: 2412-2414
  ет-lactamase signal peptide: 1554-1622
  ет-lactamase mature protein: 1623-2411