* [FAQ] DNA SMART ChIP-Seq kit º¸±â

ChIP Elute KitÀº chromatin immunoprecipitation (ChIP) ¹æ¹ýÀ¸·Î ¾ò¾îÁø DNA¸¦ ȸ¼ö, Á¤Á¦ÇÒ ¼ö ÀÖ´Â Á¦Ç°ÀÌ´Ù. º» Á¦Ç°À» »ç¿ëÇϸé Single-stranded DNA (ssDNA)¸¦ ¾à 1½Ã°£¸¸¿¡ ȸ¼öÇÒ ¼ö ÀÖÀ¸¸ç, ȸ¼öµÈ DNA´Â DNA SMART ChIP-Seq Kit (code 634865)¿¡ ¹Ù·Î Àû¿ëÇÒ ¼ö ÀÖ´Ù.

DNA SMART ChIP-Seq KitÀº º°µµÀÇ ligation °úÁ¤ ¾øÀÌ(Ligation-independent manner), ¸ñÀû DNA ¾ç ¸»´Ü¿¡ Illumina-specific sequencing adaptor¸¦ ºÎ°¡ÇÏ¿© Illumina platform¿¡ ÀûÇÕÇÑ DNA Library¸¦ Á¦ÀÛÇÏ´Â Á¦Ç°ÀÌ´Ù. Chromatin Immunoprecipitation(ChIP)À¸·ÎºÎÅÍ È¸¼öÇÑ DNA´Â ¾çÀÌ ¸Å¿ì Àû±â ¶§¹®¿¡ Chromatin Immunoprecipitation-sequencing(ChIP-Seq)¿¡ Àû¿ëÇϱⰡ ¸Å¿ì ¾î·Æ´Ù. DNA SMART ChIP-Seq KitÀº ligation °úÁ¤ ¾øÀÌ, 100 pg - 10 ngÀÇ ¼Ò·®ÀÇ DNA »ùÇ÷κÎÅÍ Illumina platform¿ë sequencing library¸¦ Á¦ÀÛÇÒ ¼ö ÀÖ´Ù. ¶ÇÇÑ Àý´Ü ¶Ç´Â ºÐÇØµÈ double strand DNA(dsDNA) »Ó¸¸ ¾Æ´Ï¶ó single-strand DNA(ssDNA)µµ ÀÌ¿ëÇÒ ¼ö Àֱ⠶§¹®¿¡ ssDNA·ÎºÎÅÍ dsDNA·Î ¸¸µé±â À§ÇÑ º°µµÀÇ °úÁ¤ÀÌ ÇÊ¿ä ¾ø´Ù. º» Á¦Ç°À» ÀÌ¿ëÇÏ¸é ¾à 4½Ã°£¸¸¿¡ È¿À²ÀûÀ¸·Î library Á¦ÀÛÀÌ °¡´ÉÇÏ´Ù.

º» Á¦Ç°Àº Clontech ƯÇãÀÇ SMART ±â¼úÀ» Àû¿ëÇÑ »õ·Î¿î Á¦Ç°À¸·Î DNA ÁÖÇüÀ¸·ÎºÎÅÍ DNA¸¦ ÇÕ¼ºÇÒ ¼ö ÀÖ´Ù (DNA dependent DNA polymerase). DNA SMART ChIP-Seq KitÀÇ ÇÙ½ÉÀº ±Ø¼Ò·®ÀÇ »ùÇà (low-input sample)·ÎºÎÅÍ ligation °úÁ¤ ¾øÀÌ Illumina¿ë sequencing adaptor¸¦ ºÎ°¡ÇÒ ¼ö ÀÖ´Ù´Â Á¡ÀÌ´Ù.
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±×¸² 1. This single-tube workflow. allows users to generate Illumina-compatible libraries for ChIP-seq experiments. After library size selection and purification, the total time from input DNA to ChIP-seq library is approximately four hours.


Ç¥ 1. The DNA SMART ChIP-Seq Kit uses a combined size selection and clean-up step after library amplification by PCR. Compared to other protocols that perform size selection before PCR amplification, post-PCR size selection results in a higher yield while maintaining the quality of the libraries.


Ç¥ 2. The DNA SMART ChIP-Seq Kit has the sensitivity to generate sequencing libraries from very small amounts of fragmented DNA. The number of unique, non-duplicate reads is high across all input levels, and the number of peaks identified is similar across input amounts.
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- DNA SMART ChIP-Seq Components
- Indexing Primer Set HT for Illumina
- SeqAmp DNA Polymerase

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Note

634865

DNA SMART ChIP-Seq Kit - 12

the reagents to generate up to 12 indexed libraries using a single forward primer and the 12 reverse indexing primers

634866

DNA SMART ChIP-Seq Kit - 48 A

generate up to 48 indexed libraries using the first 4 forward primers and the 12 reverse indexing primers

634867

DNA SMART ChIP-Seq Kit - 48 B

generate up to 48 indexed libraries using the second 4 forward primers and the 12 reverse indexing primers