ÇÁ·ÎÅäÄÝÀÇ À¯¿¬¼º°ú È¿À²ÀûÀ̸ç, ƯÀÌÀûÀ¸·Î glycoproteinÀ» ³óÃà
- À¯¿¬ÇÔ - gravity flow³ª FLPC »ç¿ë°¡´É
- ¿ì¼öÇÑ ¼öÇà·Â - glycoprotein¿¡ ´ëÇÑ Æ¯ÀÌÀû °áÇÕÀº Áõ°¡ & ºñƯÀÌÀû °áÇÕÀº °¨¼Ò
- ƯÀ̼º - Àû°Å³ª ¸¹ÀÌ Á¸ÀçÇÏ´Â Ç÷û glycoproteinÀ» ³óÃà
- ½Å¼ÓÇÔ - 90 ºÐ ¾È¿¡ glycoproteinÀ» ³óÃà
Glycoprotein Enrichment ResinÀº °£´ÜÇÑ gravity flow columnÀ̳ª medium pressure ¹æ¹ýÀ» »ç¿ëÇÏ¿© human serum°ú °°Àº º¹ÀâÇÑ ½Ã·á·ÎºÎÅÍ glycoproteinÀ» ºü¸£°í È¿À²ÀûÀÌ¸ç Æ¯ÀÌÀûÀ¸·Î ³óÃàÇÒ ¼ö ÀÖ´Â boronic acid ±â¹ÝÀÇ resinÀÌ´Ù.
°æÀï»çº¸´Ù ´õ ƯÀÌÀûÀÌ´Ù.
º» resinÀ» °æÀï»çÀÇ resin°ú ºñ±³ÇÏ¿´À» ¶§, serum glycoproteinÀÇ Æ¯ÀÌÀû °áÇÕÀº Áõ°¡Çϰí nonglycosylated serum proteinsÀÇ ºñƯÀÌÀû °áÇÕÀº °¨¼ÒÇÏ´Â °ÍÀ¸·Î ³ªÅ¸³µ´Ù (Figure 1).

Figure 1. Clontech¡¯s Glycoprotein Enrichment Resin demonstrates more effective glycoprotein enrichment than a competitor¡¯s resin. Glycoprotein Enrichment Resin provides decreased leakage of glycosylated proteins (IgG) in the flowthrough fraction (Lanes 2 & 5),and increased binding of glycosylated proteins (IgG) to the column (Lanes 3 & 6) compared to Competitor S¡¯s resin, as demonstrated by Western blotting with an antibody to human IgG.
Components & Storage Conditions
°¢ Á¦Ç° ±¸¼º¹°°ú º¸°üÁ¶°ÇÀº Certificate of Analysis ¸¦ ÂüÁ¶ÇϽʽÿÀ.
References
1. Glycoprotein Enrichment and Detection (July 2008) Clontechniques XXIII(3):6-8.