Á¦Ç° ¼³¸í
º» Á¦Ç°Àº TaKaRa Taq¢âÀÇ ÀÔÁõµÈ ¼º´É°ú È¿À²ÀûÀÎ 3¡¯ -> 5¡¯ exonucleaseÀÇ ±³Á¤´É·ÂÀ» ¸ðµÎ °®Ãá °íÈ¿À², °í°¨µµÀÇ DNA polymeraseÀÌ´Ù. ±æÀ̰¡ ±ä ´ÜÆíÀÇ ÁõÆø¿¡µµ ÀûÇÕÇϸç ÀϹÝÀûÀÎ PCR application¿¡¼­ ±âÁ¸ÀÇ Taq DNA polymeraseº¸´Ù ³ôÀº ÁõÆøÈ¿À², ³·Àº mismatchÀ²À» ½ÇÇöÇÏ¿´´Ù.
TaKaRa Ex Taq¢çÀº ¹ÝÀÀ¿ë buffer (Mg2+ Æ÷ÇÔ ¶Ç´Â Mg2+ º°µµÁ¦°ø) ¿Í dNTP mixture°¡ ÇÔ²² Á¦°øµÈ´Ù.
³»¿ë (250 U)
TaKaRa Ex Taq¢ç (Code RR001A)
1. TaKaRa Ex Taq (5 U/§¡) 250 U
2. 10¡¿ Ex Taq Buffer (20 mM Mg2+ plus) 1 §¢
3. dNTP Mixture (°¢ 2.5 mM) 800 §¡

TaKaRa Ex Taq¢ç(Mg2+ free Buffer) (Code RR001AM)
1. TaKaRa Ex Taq (5 U/§¡) 250 U
2. 10¡¿ Ex Taq Buffer (Mg2+ free) 1 §¢
3. MgCl2(25 mM) 1 §¢
4. dNTP Mixture (°¢ 2.5 mM) 800 §¡
º¸Á¸ -20¡É
Çü»ó

20 mM

Tris-HCl (pH8.0)

100 mM

KCl

0.1 mM

EDTA

1 mM

DTT

0.5%

Tween 20

0.5%

NP-40

50%

Glycerol

÷ºÎ dNTP Mixture Á¶¼º*

³óµµ

°¢ 2.5 mM

Çü»ó

¼ö¿ë¾× (³ªÆ®·ý¿°), pH7~9

¼øµµ

°¢ 98% ÀÌ»ó

*dATP, dCTP, dGTP, dTTPµî ¸ô È¥ÇÕ¹°·Î Èñ¼®ÇÏÁö ¾Ê°í ±×´ë·Î PCR¹ÝÀÀ¿¡ ÀÌ¿ëÇÒ ¼ö ÀÖ´Ù.
PCR »ê¹°¿¡ ´ëÇÏ¿©
TaKaRa Ex Taq¢çÀ» ÀÌ¿ëÇÏ¿© ÁõÆøÇÑ PCR »ê¹°ÀÇ ´ëºÎºÐÀº 3¡¯ ¸»´Ü¿¡ A°¡ 1¿°±â ºÎ°¡µÇ¾î ÀÖ´Ù. µû¶ó¼­ ÀÌ PCR »ê¹°À» ±×´ë·Î T-vector pMD20 (Code 3270) ¶Ç´Â T-vector pMD19 (Simple) (Code 3271)¿¡ cloningÇÒ ¼ö ÀÖ´Ù. ¶ÇÇÑ ¸»´Ü ÆòȰȭ ¹× ÀλêÈ­ ÈÄ, ÆòȰ ¸»´Ü vector¿¡ cloningÇÒ ¼öµµ ÀÖ´Ù.
°ü·ÃÁ¦Ç°
- ºñƯÀÌÀûÀÎ ÁõÆøÀ» ¹æÁö: TaKaRa Ex Taq¢ç Hot Start Version
- 2X PCR master mix: Premix Taq¢â (Ex Taq Version 2.0) (500 ¥ìl¡¿6 tube)
- ¿ªÀü»ç¹ÝÀÀÀ» À§ÇÑ all-in-One kit: PrimeScript¢â 1st strand cDNA Synthesis Kit
- 5x premix ¿ªÀü»ç ½Ã¾à: PrimeScript¢â IV 1st strand cDNA Synthesis Mix
- ´Ù¾çÇÑ »ùÇÃÀÇ DNA Á¤Á¦¿¡ ÃÖÀû: TaKaRa MiniBEST Universal Genomic DNA Extraction Kit
- ¿Ã¶ó¿îµå Agarose: SeaKem¢ç LE Agarose
- 5ºÐ¸¸¿¡ Àü±â¿µµ¿ ¿Ï·á! Precast gel system: FlashGel¢â System
- ÄÄÆÑÆ®ÇÑ 96 well gradient PCR ±â±â : Clontech PCR Thermal Cycler GP