TransIT-2020 is a broad spectrum transfection reagent that provides superior transfection of plasmid DNA into mammalian cells. TransIT-2020 is suitable for both transient and stable transfection and works well in typically hard-to-transfect cell lines. TransIT-2020 is comprised of animal-origin free components and is serum compatible, eliminating the need for any culture media change after transfection.
Materials Supplied
Product No. Quantity Number of Transfections
V5404 1 ¡¿ 0.4 ml Up to 160
V5400 1 ¡¿ 1.0 ml Up to 400
V5405 5 ¡¿ 1.0 ml Up to 2000
V5406 10 ¡¿ 1.0 ml Up to 4000
SPECIFICATIONS
Storage Store TransIT-2020 Reagent at -20¡ÆC.
Before each use, warm to room temperature and vortex gently.
Stability/ Guarantee 6 months from the date of purchase,
when properly stored and handled.
Data

High Performance Plasmid Transfection. Primary Human Small Epithelial Cells (HSAEpic) were transfected using TransIT-2020 and an EGFP expression plasmid (4:1 reagent-to-DNA ratio). Images were taken 24 hours post-transfection using a Zeiss axiovert inverted fluorescence microscope.
Superior Gene Expression in a Broad Spectrum of Cell Types.
Experimental Details: Luciferase gene expression was compared in HEK 293, K562, CHO-K1, and primary MEF cells transfected with a luciferase expression plasmid using TransIT-2020 (Mirus Bio, 3:1 reagent to DNA ratio), FuGENE HD (Roche, 7:2 reagent to DNA ratio), Lipofectamine 2000 (Invitrogen, 5:2 reagent to DNA ratio), and Lipofectamine 2000 CD (Invitrogen, 5:2 reagent to DNA ratio). Transfections were performed in 24-well plates using 0.5§¶ of plasmid DNA per well. The optimal level of each transfection reagent was determined empirically and all reagents were used according to manufacturer's protocol. Cells were harvested at 24 hours post-transfection and assayed for luciferase activity. Error bars represent the standard deviation of triplicate wells.