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Suspension CHO cell용 대량 단백질 발현 시스템

CHOgro® Expression System

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제조사 제품코드 제품명 용량 가격
(부가세별도)
비고 사용자매뉴얼
Mirus
MIR 6260
CHOgro® Expression System
관련학술 관심상품등록 구매하기
1 Kit
947,000원  chogro-expression-system-protocol.pdf
Mirus
MIR 6200
CHOgro® Expression Medium
관련학술 관심상품등록 구매하기
1 L
180,000원  chogro-expression-system-protocol.pdf
Mirus
MIR 6210
CHOgro® Complex Formation Solution
관련학술 관심상품등록 구매하기
100 ml
45,000원  chogro-expression-system-protocol.pdf
Mirus
MIR 6230
Poloxamer 188 Solution
관련학술 관심상품등록 구매하기
100 ml
45,000원 
Mirus
MIR 6240
L-Glutamine Solution
관련학술 관심상품등록 구매하기
100 ml
45,000원 
Mirus
MIR 6250
Human IgG1 Expression Control
관련학술 관심상품등록 구매하기
100 ug
541,000원  human-igg1-expression-control-qrp.pdf
Mirus
MIR 5740
TransIT-PRO® Transfection Reagent
관련학술 관심상품등록 구매하기
1 ml
631,000원 
Mirus
MIR 5750
TransIT-PRO® Transfection Reagent
관련학술 관심상품등록 구매하기
10 ml
5,053,000원 

제품 설명
CHOgro® Expression System은 suspension CHO Cell을 이용하여 대량 단백질 발현에 최적인 제품이다. Transfection 시약과 배지가 모두 포함된 all-in-one kit이며, 다른 culture system을 사용하고 있는 연구자도 transfection 24시간 전에 배지 교체하여 간편하게 높은 단백질 발현을 경험할 수 있다.
  • 셋업이 간편: 대량 단백질 생산에 최적인 프로토콜 제공
  • Cell clumping 없이 균일한 단백질 발현 및 높은 viability 실현
  • Animal origin free, hydrolysate-free
  • cGMP (국제의약품 품질 기준) 조건에서 생산
CHOgro® Expression System 구성

구성품

용량

별도구매 가능

TransIT-PRO® Transfection Reagent

1 ml

MIR 5740

CHOgro® Expression Medium

2 × 1000 ml

MIR 6200

CHOgro® Complex Formation Solution

100 ml

MIR 6210

Poloxamer 188 Solution

100 ml

MIR 6230

L-Glutamine Solution

100 ml

MIR 6240

Media에 따른 cell density, viability 비교

Figure 1. Suspension CHO cells grow to high density in the CHOgro® Expression Medium. Tripli­cate flasks of FreeStyle™ CHO-S cells were seeded in CHOgro® Expression Medium (red line) or FreeStyle™ CHO Expression Medium (blue line) at a cell density of 0.5 x 106 cells/㎖. Cell counts (solid line) and viability (dotted line) were measured daily using a Guava easyCyte™ 5HT flow cytometer.
Expression system에 따른 단백질 발현 비교

Figure 2. CHOgro® Expression Medium yields multi-fold increases in antibody titer. Human IgG1 was produced by transient transfection using TransIT-PRO® (1:1), FreeStyle™ MAX (1.25:1.25) or 25kDa linear PEI (6:1) transfection reagents according to the manufacturers’ or published protocol (reagent:DNA ratio). FreeStyle™ CHO-S cells were transfected using 1㎍ plasmid DNA per milliliter of culture and cell densities of 2 x 106 cells/㎖ or 1 x 106 cells/㎖ for the CHOgro® Expression Medium (red bars) or FreeStyle™ Expression Medium (blue bars). Antibody levels were also analyzed from day 6 clarified supernatants using a human IgG ELISA.
간편한 culture system 변경과 발현율 증가

Figure 3. Media exchange leads to higher protein production. Free-Style™ CHO-S cells were cultured in FreeStyle™ CHO Expression Medium or CHOgro® Expression Medium and 24 hours prior to transfection a subset of the cells grown in FreeStyle™ CHO Expression Medium were spun down and exchanged with 100% fresh CHOgro® Expression Medium. The cells were allowed to grow and adapt for 24 hours prior to transfection with FreeStyle™ MAX or TransIT-PRO® transfection reagents according to the manufacturers’ protocol and a hIgG1 encoding construct. (A) Workflow schematic of media exchange of CHO-S cells from FreeStyle™ CHO Expression Medium to CHOgro® Expression Medium (black arrow) or the normal CHOgro® Expression System (red arrow) (B) Day 6 supernatants were clarified and analyzed using a human IgG ELISA. Data is normalized to the complete CHOgro® Expression System (red bar).
Human IgG1 Expression Control
포유류 세포에서 항체 발현 확인용 control로, 대량 항체 생산에 적합한 제품이다.

Figure 4.Human IgG1 Expression Control is an Optimized Premix of Heavy and Light Chain Constructs. The Human IgG1 Expression Control is an equimolar premix of proprietary human IgG1 heavy and light chain expression constructs with antibody expression driven by CMV promoters. This product incorporates DNA2.0 IP-Freeⓒ vector technology.
관련 제품
고발현 transfection reagent TransIT-PRO® Transfection Reagent 정보 자세히 보기
Endotoxin 제거를 통한 단백질 발현 향상 MiraCLEAN® Endotoxin Removal Kit 자세히보기
Transfection 효율 확인용 Label IT® Plasmid Delivery Control, Cy® 3 자세히보기

Keyword :
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포유류 세포 단백질 발현
Recombinant Protein의 Refolding
> Chaperonin GroE
Suspension CHO cell용 대량 단백질 발현 시스템
> CHOgro® Expression System
단백질 disulfide Bond의 재조합에
> Corystein™(Purothionin)
무세포 단백질 합성계
> Human Cell-Free Protein Expression System
Human cell (HEK293)에서 단백질을 고발현
> pHEK293 Ultra Expression Vector
단백질 disulfide Bond의 재조합에
> Protein Disulfide-isomerase(PDI)
Inclusion Body의 Refolding 조건 검토에 최적 Kit
> Refolding CA Kit
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