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제품명/제품코드
사이트내검색
Recombinant Lentivirus 생산에 최적

TransIT®-Lenti Transfection Reagent

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제조사 제품코드 제품명 용량 가격
(부가세별도)
비고 사용자매뉴얼
Mirus
MIR 6600
TransIT®-Lenti Transfection Reagent
관련학술 관심상품등록 구매하기
1.5 ㎖
510,000원 
857,000원
가격할인
09.11 ~ 10.31
transit-lenti-reagent-protocol.pdf
Mirus
MIR 6603
TransIT®-Lenti Transfection Reagent
관련학술 관심상품등록 구매하기
0.3 ㎖
172,800원 
216,000원
가격할인
09.11 ~ 10.31
Mirus
MIR 6604
TransIT®-Lenti Transfection Reagent
관련학술 관심상품등록 구매하기
0.75 ㎖
432,800원 
541,000원
가격할인
09.11 ~ 10.31
Mirus
MIR 6605
TransIT®-Lenti Transfection Reagent
관련학술 관심상품등록 구매하기
5 x 1.5 ㎖
2,982,400원 
3,728,000원
가격할인
09.11 ~ 10.31
Mirus
MIR 6606
TransIT®-Lenti Transfection Reagent
관련학술 관심상품등록 구매하기
10 x 1.5 ㎖
5,485,600원 
6,857,000원
가격할인
09.11 ~ 10.31
Mirus
MIR 6620
TransduceIT™ Transduction Reagent
관련학술 관심상품등록 구매하기
1 ㎖
36,000원 
45,000원
가격할인
09.11 ~ 10.31
transduceit-reagent-pds.pdf

  • High Performance - Provide up to eight-fold higher functional titers
  • Simple Protocol - No media change required, single harvest
  • Animal Origin Free - Regulatory friendly
제품설명
TransIT®-Lenti Transfection Reagent는 adherent HEK 293T 세포에 packaging vector와 lentiviral vector를 효율적으로 co-transfection하여 recombinant lentivirus를 생산할 수 있는 transfection 시약이다.
TransduceIT™ Transduction Reagent는 hexadimethrine bromide 수용액으로 retroviral/lentiviral transduction 효율을 높이고자 할 때 사용한다.
보존 - 20 ℃
적용예
1. High Functional Titers with TransIT®-Lenti Transfection Reagent.


Adherent 293T/17 cells were transfected in a 6-well plate with pLKO.1-puro-CMV-TurboGFP™ transfer vector and the Lentivirus Packaging Mix powered by MISSION® (1:1 ratio, 2 μg/well) with the following reagents: TransIT®-Lenti (3:1, vol:wt), Lipofectamine™ 2000 (3:1), Lipofectamine™ 3000 (3:1:1), 25 kDa PEI (6:1), or CaPO4 precipitation (4 μg pDNA/well). The supernatant was harvested, filtered (0.45 μm), and titered using 293T/17 cells. Lentivirus transductions were performed in the presence of 8 μg/ml TransduceIT™ and GFP expression was measured 72 hours post-transduction using guava easyCyte™ 5HT Flow Cytometer. Error bars represent triplicate transfection complexes titered individually. Functional titers were calculated using virus dilutions with less than 20% GFP positive cells.

2. High Transduction Efficiency with Unconcentrated Lentivirus Using TransIT®-Lenti.

(A) Lentivirus was produced with the TransIT®-Lenti Transfection Reagent (3:1, vol:wt) or Lipofectamine™ 2000 using the MISSION® vectors (pLKO.1-puro-CMV-TurboGFP™ transfer vector and the Lentivirus Packaging Mix powered by MISSION®). The supernatant was harvested, filtered (0.45 μm), and frozen. Lentivirus transductions were performed 5 days post-plating with iCell® Motor Neurons (Cellular Dynamics International). For bothTransIT-Lenti and Lipofectamine™ 2000, one microliter of unconcentrated supernatant was added per well of a 96-well plate. GFP efficiency was measured 72 hours post-transduction using guava easyCyte™ 5HT Flow Cytometer. Error bars represent the SEM of duplicate wells. (B) iCell® Motor Neurons were plated in Ibidy 35mm dishes and transduced withlentivirus produced using the TransIT®-Lenti Transfection Reagent and MISSION® vectors. Images were captured at 72 hours post-transduction with a Zeiss Axiovert S100 inverted fluorescence microscope using a 63X objective under oil.

Keyword :
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진핵세포 용 유전자 도입 시약
> Cell line별 transfection reagent 선택가이드
> CalPhos Mammalian Cell Transfection 시약
> In Vivo Luciferase Imaging Kit
> Ingenio Electroporation 관련 제품
핵산도입이 곤란한 포유동물 세포 용 Electrophorat..
> Ingenio Electroporation Kit & Solution
DNA 시료로 부터 endotoxin 제거
> MiraCLEAN Endotoxin Removal Kit
Mouse 생체 내 세포 용 유전자 도입 시약
> TransIT Hydrodynamic Delivery System
Recombinant Lentivirus 생산에 최적
> TransIT®-Lenti Transfection Reagent
범용적인 Transfection 시약(동물 유래 성분 free)
> TransIT-2020 Transfection Reagent
> TransIT-293
> TransIT-BrCa Transfection Reagent
> TransIT-CHO
> TransIT-HeLaMONSTER
> TransIT-Insect Transfection Reagent
> TransIT-Jurkat
> TransIT-Keratinocyte
범용적인 Transfection 시약
> TransIT-LT1 Transfection Reagent
> TransIT-mRNA Transfection Kit
> TransIT-Neural
> TransIT-Oligo
> TransIT-PRO Transfection Kit
Plasmid DNA와 siRNA/miRNA를 효과적으로 delivery
> TransIT-X2 Dynamic Delivery System
Mammalian cell 용 고효율 · 저독성 Transfection ..
> Xfect Transfection 시약
Mous ES 세포에 최적화된 Transfection 시약
> Xfect mESC Transfection Reagent
> Xfect Protein Transfection Reagent
One Tube=One Transfection format
> Xfect Single Shots (Midi)
mRNA, sgRNA, siRNA등 다양한 RNA transfection에 ..
> Xfect™ RNA Transfection Reagent
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