- SMART¹ýÀ» ÀÌ¿ëÇÑ NGS¿ë Strand-specific NGS Library Á¦ÀÛ
- 100pg-100ngÀÇ RNA (purified Poly A+ RNA ¶Ç´Â rRNA depleted RNA)¿¡¼ Library Á¦ÀÛ
- Illumina¢ç sequencer¿¡ ÀûÇÕÇÑ index¿Í adaptor Àû¿ë
- 5½Ã°£ À̳»¿¡ RNA-seq library Á¦ÀÛ °¡´É
- GC-, AT-rich template¿¡ ÃÖÀûÀÎ PCRÈ¿¼Ò ¹× rRNA Á¦°Å½Ã¾à Æ÷ÇÔ
SMARTer¢ç Stranded RNA-Seq Kit´Â 100pg~10ngÀÇ RNA¸¦ ÀÌ¿ëÇÏ¿© Àü»çüÀÇ strand informationÀ» À¯ÁöÇÏ¸é¼ Illumina¢ç sequencing platforms¿¡
Àû¿ëÇÒ ¼ö ÀÖ´Â library¸¦ Á¦ÀÛÇÒ ¼ö ÀÖ´Â ½Ã¾àÀÌ´Ù. º» Á¦Ç°Àº random primer ¹æ¹ýÀ» ÀÌ¿ëÇÏ¿© polyA+ RNA¿Í rRNA-removed total RNA·ÎºÎÅÍ NGS¿ë
cDNA library¸¦ Á¦ÀÛÇϸç, mammalian RNA (poly A+ RNA)¿Í ¹ÚÅ׸®¾Æ »ùÇà (non-poly A+ RNA)¸¦ ÀÌ¿ëÇÒ ¼ö ÀÖ´Ù.
±âÁ¸ÀÇ Clonetch SMARTerÀÇ ¿ø¸®¸¦ ÀϺΠº¯ÇüÇÏ¿© ½ÇÇè °úÁ¤ Áß¿¡, Illumina¢ç index¿Í adaptor¸¦ °áÇÕÇϱ⠶§¹®¿¡ º°µµÀÇ Library preparation kit°¡
ÇÊ¿äÇÏÁö ¾ÊÀ¸¸ç, ºÒ°ú 4½Ã°£ ¸¸¿¡ Illuimina¢ç sequencing¿¡ Àû¿ë °¡´ÉÇÑ indexed library Á¦ÀÛÀÌ °¡´ÉÇÏ´Ù.
º» Á¦Ç° SMARTer¢ç Stranded Total RNA Sample Prep Kit - Low Input Mammalian (Code 634861)Àº ±âÁ¸ÀÇ SMARTer¢ç Stranded RNA-Seq Kit (Code 634836)¿¡
rRNA depletionÀ» À§ÇÑ RiboGone-Mammalian (Code 634847)À»
Æ÷ÇÔÇÏ°í ÀÖ´Ù.
±×¸² 1. NGS Work flow for the SMARTer¢ç Stranded RNA-Seq Kit
Applications
- Illumina¢ç-specific NGS library production that retains strand information.
- RNA-Seq on Illumina¢ç platforms for both mammalian and prokaryotic samples.
- Coding and non-coding transcript information
±¸¼ºÇ° (ÀÚ¼¼ÇÑ ±¸¼ºÇ°Àº CoA ¶Ç´Â ¸Å´º¾ó ÂüÁ¶)
SMARTer¢ç Stranded Total RNA Sample Prep Kit - Low Input Mammalian (Code 634861)
* SMARTer¢ç Stranded RNA-Seq Components
* SeqAmp¢â DNA Polymerase
* 2 x Illumina¢ç Indexing Primer Set
* RiboGone¢â - Mammalian