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High-throughput & microscale

His60 Ni Magnetic Beads

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Á¦Á¶»ç Á¦Ç°ÄÚµå Á¦Ç°¸í ¿ë·® °¡°Ý
(ºÎ°¡¼¼º°µµ)
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Clontech
635693
His60 Ni Magnetic Beads
°ü·ÃÇмú ±¸¸ÅÇϱâ
1 ml
246,000¿ø  Á¦Á¶»ç ÆäÀÌÁö·Î ¹Ù·Î°¡±â x32709
Clontech
635692
His60 Ni Magnetic Beads
°ü·ÃÇмú ±¸¸ÅÇϱâ
10 mL
1,070,000¿ø  Á¦Á¶»ç ÆäÀÌÁö·Î ¹Ù·Î°¡±â
Clontech
635665
His60 Ni Buffer Set
°ü·ÃÇмú ±¸¸ÅÇϱâ
20 ȸ
524,000¿ø  Á¦Á¶»ç ÆäÀÌÁö·Î ¹Ù·Î°¡±â x32711
Clontech
631964
Magnetic Stand
°ü·ÃÇмú ±¸¸ÅÇϱâ
1 Set
114,000¿ø  Á¦Á¶»ç ÆäÀÌÁö·Î ¹Ù·Î°¡±â

  • His-tagged proteinÀÇ ºü¸£°í °£ÆíÇÑ ºÐ¸®
  • His60 Ni°ú magnetic bead¸¦ ÀÌ¿ëÇÏ¿© °í¼øµµÀÇ ´Ü¹éÁú Á¤Á¦
  • ÀûÀº ºÎÇÇ¿¡¼­ elutionÇÏ¿© °í³óµµ Á¤Á¦ °¡´É
Á¦Ç° ¼³¸í
His60 Ni Magnetic Beads´Â ½Å¼ÓÇÏ°í °£ÆíÇÏ°Ô his-tagged proteinÀ» Á¤Á¦ÇÒ ¼ö ÀÖ´Â magnetic beads·Î, magnetic separator¸¦ ÀÌ¿ëÇØ ¹Ì¼Ò±Ô¸ðÀÇ ´Ü¹éÁúÀ» ºü¸£°í ½±°Ô ºÐ¸®ÇÒ ¼ö ÀÖ´Ù.

Highly Specific Binding & Elution
His60 Ni Magnetic Beads´Â ³ôÀº ƯÀ̼ºÀ¸·Î ÀÛÀº ºÐÀÚ·®¿¡¼­ Å« ºÐÀÚ·®±îÁö ´Ù¾çÇÑ Å©±âÀÇ his-tagged protein°ú °áÇÕÇÑ´Ù. Á¤Á¦µÈ ´Ü¹éÁúÀº ¼Ò·®ÀÇ ¿ë¾× (50-200 §¡)À¸·Î elutionÇÏ¿© °í³óµµ(~3 §·/§¢)ÀÇ »ùÇÃÀ» Á¤Á¦ÇÒ ¼ö ÀÖ´Ù. His60 Ni Magnetic Beads´Â 5% ÇöŹ¾×À¸·Î Á¦°øµÇ¸ç, §¢ ´ç 1.65§·ÀÇ ´Ü¹éÁú°ú °áÇÕÇÒ ¼ö ÀÖ´Ù.
Microscale Screening
His60 Ni Magnetic Beads¸¦ ÀÌ¿ëÇÑ microscale Á¤Á¦´Â ´Ü¹éÁúÀÇ ¹ßÇö ¼öÁØÀ» ½ºÅ©¸®´×Çϰųª protein-protein interaction ¿¬±¸¿¡ ÀûÇÕÇϸç, ¶ÇÇÑ His60 Ni ±â¼úÀÌ Á¢¸ñµÈ ClontechÀÇ His60 Ni Superflow ResinÀ» »ç¿ëÇÔÀ¸·Î½á ¿øÈ°ÇÏ°Ô ¸ñÀû ´Ü¹éÁúÀÇ Á¤Á¦¸¦ scale-up ÇÒ ¼ö ÀÖ´Ù.

±×¸². Native condition¿¡¼­ÀÇ his-tagged AcGFP1 Á¤Á¦ ¿¹ AcGFP1-6xHNÀ» ¹ßÇöÇÏ´Â E. coli lysate 400 §¡¸¦ His60 Ni Magnetic Beads¸¦ ÀÌ¿ëÇÏ¿© Á¤Á¦ÇÏ¿´´Ù.


±×¸². Denaturing condition¿¡¼­ÀÇ his-tagged AcGFP1 Á¤Á¦ ¿¹ AcGFP1-6xHNÀ» ¹ßÇöÇÏ´Â E. coli lysate 500 §¡¸¦ His60 Ni Magnetic Beads ¸¦ ÀÌ¿ëÇÏ¿© Á¤Á¦ÇÏ¿´´Ù.


±×¸². His60 Ni Magnetic Beads
º¸Á¸:£º4¡É
Components & Storage Conditions
°¢ Á¦Ç° ±¸¼º¹°°ú º¸°üÁ¶°ÇÀº Certificate of Analysis ¸¦ ÂüÁ¶ÇϽʽÿÀ.
°ü·ÃÁ¦Ç°
- Protein Á¤Á¦¸¦ À§ÇÑ Àü¿ë lysis buffer : xTractor¢â Buffer Kit
- His-tagged protein °í¹ßÇöÀ» À§ÇÑ cold shock vector system : pCold¢â vector
- Acetic acid ¾ø´Â °í°¨µµ ´Ü¹éÁú ¿°»ö½Ã¾à : TaKaRa CBB Protein Safe Stain
- ¹ü¿ëÀûÀÎ °ËÃâºÎÅÍ ÃÊ°í°¨µµ °ËÃâ±îÁö ´Ù¾çÇÑ Á¦Ç° ¶óÀξ÷ : HRP Substrate ½Ã¸®Áî
- ½Ç¿Â¿¡¼­ 30ºÐ ¿Ï·á : Western BLoT Stripping Buffer

Keyword :

His-tag À¶ÇÕ ´Ü¹éÁú Á¤Á¦
>
Spin columnŸÀÔÀ¸·Î ºü¸£°í °£ÆíÇÑ His-tag ´Ü..
Capturem¢â His-Tagged Purification
>
His-tagged protein °í¼øµµ Á¤Á¦
TALON (Cobalt) resin & column
>
ºü¸£°Ô ¼Ò¿ë·®ÀÇ his-tagged protein Á¤Á¦
TALON Magnetic Beads
>
FPLC purification - His-tagged protein
TALON (Cobalt) Superflow resin & cartridge
>
´ëÀå±ÕÀ¸·ÎºÎÅÍ His-tag À¶ÇÕ ´Ü¹éÁú ÃßÃâ¿¡,xT..
xTractor Buffer Kit
>
°í¼öÀ² Ni resin & FPLC purification
His60 (Nickel) Superflow resin & cartridge
>
High-throughput & microscale
His60 Ni Magnetic Beads
>
4¡É¿¡¼­ È°¼ºÀ» ¶ç´Â HN-tag¸¦ °®°í ÀÖ´Â ´Ü¹é..
HRV 3C Protease
>
Cell lysate¿¡¼­ His-tagged protein Á÷Á¢ Á¤Á¦
TALON (Cobalt) CellThru resin
>
Prepacked spin column
TALONSpin Column