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DNA Markers
Lonza DNA Marker´Â PCR »ê¹°À̳ª Á¦ÇÑÈ¿¼Ò ¹ÝÀÀ¹°ÀÇ ºü¸¥ »çÀÌÁî È®ÀÎÀ» À§ÇÑ Á¦Ç°À¸·Î ethidium bromide (EtBr) stainingÀ» ÅëÇØ °£ÆíÇÏ°Ô È®ÀÎ °¡´ÉÇÏ´Ù.
DNA Markers ¸ð½Äµµ
DNA Ladders
Lonza DNA Ladder´Â ºü¸£°í ½±°Ô DNA sample»çÀÌÁ È®ÀÎ ÇÒ ¼ö ÀÖ´Â Á¦Ç°À¸·Î ¼±¸íÇÑ Çػ󵵸¦ °¡Áö°í ÀÖ´Ù.
DNA Ladders ¸ð½Äµµ
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Ladder

6X Loading Buffer

Distilled Water

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1 kb DNA Marker (1 kb - 10 kb)

5.0 ¥ìl

1.0 ¥ìl

-

For a 6 ¥ìl total load volume

50 bp DNA Marker (50 bp - 2,500 bp)

5.0 ¥ìl

1.0 ¥ìl

-

50 bp DNA Marker (50 bp - 1,000 bp)

5.0 ¥ìl

1.0 ¥ìl

-

20 bp DNA Ladder (20 bp - 500 bp)

1.0 ¥ìl

2.5 ¥ìl

11.5 ¥ìl

For a 15 ¥ìl total load volume

20 bp Extended Range DNA Ladder (20 bp - 1,000 bp)

1.5 ¥ìl

2.5 ¥ìl

11.0 ¥ìl

100 bp DNA Ladder (100 bp - 1,000 bp)

1.6 ¥ìl

2.5 ¥ìl

10.9 ¥ìl

100 bp Extended Range DNA Ladder (100 bp - 3,000 bp)

1.5 ¥ìl

2.5 ¥ìl

11.0 ¥ìl

500 bp DNA Ladder (500 bp - 8,000 bp)

1.5 ¥ìl

2.5 ¥ìl

11.0 ¥ìl

DNA QuantLadder (100 bp - 1,000 bp)

2.5 ¥ìl

2.5 ¥ìl

10.0 ¥ìl

DNA Reverse QuantLadder (100 bp - 1,000 bp)

2.5 ¥ìl

2.5 ¥ìl

10.0 ¥ìl

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EtBrº¸´Ù ³ôÀº °¨µµ·Î staining °¡´ÉÇÑ GelStar Nucleic Acid Stain ¹Ù·Î°¡±â