SYBR® Green Nucleic Acid Gel Stain series
특징
전기영동 예
1. DNA 전기영동: SYBR® Green I Stain과 EtBr의 감도 비교

DNA samples (pBR322 Msp I digest) ranging from 1 to 200 ng per lane were separated on a 10 cm x 16 cm x 0.1 cm, 4% vertical MetaPhor Agarose gel prepared in 1X TBE Buffer. The gel war ren for 1 hours at 488V/cm. Following electrophoresis the gel was divided into two, and one half was stained with 1 ㎍/㎖ ethidium bromide while the other was stained with SYBR® Green I Stain (1:10,000 dilution of stock). Detection was achieved with standard 300 nm UV transillumination.

2. RNA 전기영동: SYBR® Green II Stain과 EtBr의 감도 비교

Samples of E. coli total RNA were denatured using the following denaturants:
Lane A: Formaldehyde/ Formamide; Lane B: Formamide; Lane C: Glyoxal. Samples were loaded at 2 ㎍/lane for the formaldehyde/formamide and formamide only denatured samples, and 4 ㎍/lane for the glyoxal denatured samples. Reliant™ RNA precast Agarose gels were run at 7 V/cm for 40 minutes in 1X MOPS Buffer and post stained with SYBR Green II® gel stain and photo graphed on the Clare Chemical Research, Inc., Dark Reader® Transilluminator.
관련제품
- No.1 agarose: SeaKem® LE Agarose
- 사용이 간편한 premade buffer: Lonza AccuGene® Buffer 시리즈
- Gradient PCR 기기: TaKaRa PCR Thermal Cycler Touch®
- 내열성 gel maker set를 포함한 디지털 전기영동 장치: Mupid®-One