• If CMV promoters are silenced in your cell type, try EF-1 alpha
• High-titer lentiviral vector system
• Constitutive expression
• Robust, long-term expression, in the broadest range of cell types

Advantages of the EF-1 Alpha Promoter

Lenti-X¢â lentiviral vector systems with EF-1 alpha promoters allow you to achieve robust, constitutive, long-term expression of your gene of interest in cell types in which CMV promoters are often silenced, such as hematopoietic and stem cells. The EF-1 alpha promoter, which offers a broad host range, is derived from the human EEF1A1 gene that expresses the alpha subunit of eukaryotic elongation factor 1 (1, 2).

Expression Options of Lentiviral Vectors with an EF-1 Alpha Promoter

The Lenti-X Expression System (EF1a Version) is a complete system that includes the pLVX-EF1a-IRES-Puro Vector (Figure 1), and our 4th Generation Lentiviral Packaging System. Alternatively, you can express your protein fused to a red (mCherry or DsRed-Monomer) or green (AcGFP1) fluorescent protein, or express your protein and a fluorescent protein separately from an internal ribosome entry site (IRES).


Figure 1. Lentiviral Vector Systems with an EF-1 Alpha Promoter.

References

1. Uetsuki, T., Naito, A., Nagata, S., and Kaziro, Y. (1989). Isolation and Characterization of the Human Chromosomal Gene for Polypeptide Chain Elongation Factor-1. J. Biol. Chem. 264:5791?5798.
2. Kim, D., Uetsuki, T., Kaziro, Y., Yamaguchi, N. and Sugano, S. (1990).Use of the human elongation factor 1¥á promoter as a versatile and efficient expression system. Gene 91:217?223.

Components

Lenti-X¢â Expression System (EF1a Version) (Cat. No. 631253)

• pLVX-EF1¥á-IRES-Puro Vector
• Lenti-X¢â Packaging single shots
• Lenti-X¢â GoStix Plus

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LS&B47
: http://cms.takara.co.kr/file/lsnb/47-34-35.pdf