EF-1 Alpha Promoter Vectors

Human elongation factor-1 alpha (EF-1 alpha) is a constitutive promoter of human origin that can be used to drive ectopic gene expression in various in vitro and in vivo contexts (1). EF-1 alpha is often useful in conditions where other promoters (such as CMV) have diminished activity or have been silenced (as in embryonic stem cells; 2).
EF-1 alpha promoter vectors are available in multiple formats for either plasmid or lentiviral delivery:

• Fluorescent protein fusion vectors
• IRES vectors for coexpression of 2 genes
• Vectors with highly immunoreactive Myc- or HA-tags that can be used to confirm Matchmaker interactions in mammalian cells

Figure 1. EF-1 alpha expression vectors for many applications. Plasmid and lentiviral vector choices are available carrying the EF-1 alpha promoter. IRES: internal ribosome entry sequence; FP1: fluorescent protein (AcGFP1, DsRed-Monomer, or mCherry); FP2: fluorescent protein (mCherry or ZsGreen1); MCS: multiple cloning site

Storage Conditions

-20”ĘC

References

1.Teschendorf, C., et al. (2002) Anticancer Res. 22(6A):3325-3330. 2.Kim S, et al. (2007) Stem Cells Dev. 16(4):537-45. 3.Wang, et al. (2008) Stem Cells Dev. 17:279-289.