HIV-1부터 유래된 lentivirus vector는 primary culture, 비분열세포, 줄기세포 및 신경세포를 포함한 거의 모든 포유류 세포에 유전자를 도입할 수 있다.

최고 효율의 Lentivirus vector
Clontech의 모든 Lenti-X™ vector (Figure 1)는 lentivirus LTR과 packaging 서열 외에, 바이러스 역가 또는 복제 등을 크게 향상시키는 특별한 요소들을 가지고 있다.
Figure 1. Lenti-X™ Expression Systems and Vectors. Lenti-X™ vectors contain essential packaging sequences (lentiviral LTRs, the Ψ packaging sequence, etc.) and additional elements that facilitate packaging and/or boost transgene expression. The Lenti-X™ Expression System (pLVX-Puro Vector) is designed for constitutive expression of a gene of interest and provides a puromycin selectable marker. The Lenti-X™ Bicistronic Expression Systems allow your protein and a selectable marker to be coexpressed from a single mRNA transcript.
Lentiviral Packaging Systems-3가지 옵션

고역가의 Lentivirus를 제작
Lenti-X™ Packaging Single shots

Lenti-X™ Packaging Single shots은 렌티바이러스 패키징 필수 구성 유전자가 최적의 비율로 포함되어 바이러스 생산을 극대화하며, 또한 이 구성요소들이 각각의 분리된 vector로 되어 있어 바이러스 자가 복제 가능성을 효과적으로 억제하여 안정성을 확보했다.

1. VSV-G lentiviral packaging-표준의 일반적이고 가장 선호되는 Lenti-X™ Packaging Single shots (VSV-G) (Code 631275) 은 VSV-G pseudotyped lentivirus를 제작하는데 이용되며 거의 모든 종류의 세포에 감염시킬 수 있다.

2. Ecotropic lentiviral packaging - Lenti-X™ Packaging Single shots (Ecotropic) (Code 631278) 은 MLV ecotropic envelope glycoprotein을 가진 lentivirus를 생산하는데 이것은 mouse cell이나 rat cells에 trasnsduction시 유용하다.

3. Lenti-X™ Packaging Single shots (Integrase Deficient) (Code 631277) 은IDLV generation을 위한 4세대 lentiviral packaging system 전용으로 Lenti-X™ 293T 세포에 형질전환해서 최대 역가titer의 lentivirus를 생산할 수 있도록 최적화된 비율로 5개의 plasmid를 조합해 놓은 시스템이다.

Figure 2. Highly efficient transduction. 293T cells were infected with the indicated volumes (μl) of a Lenti-X™ supernatant generated using the Lenti-X™ Packaging System and the pLVX-Puro vector. After puromycin selection, resistant colonies were stained with crystal violet.
Components & Storage Conditions
각 제품 구성물과 보관조건은 Certificate of Analysis 를 참조하십시오.
제품 별 vector 정보

Vector명

정보

pLVX-Puro Vector (#632164 구성품)

Vector map

Sequence (txt.)

pLVX-EF1alpha-IRES-Puro Vector (#631253 구성품)

Vector map

Sequence (txt.)

pLVX-IRES-Neo vector (#632181 구성품)

Vector map

Sequence (txt.)

pLVX-IRES-Hyg Vector (#632182 구성품)

Vector map

Sequence (txt.)

pLVX-IRES-Puro Vector (#632183 구성품)

Vector map

Sequence (txt.)
References
1. Zufferey, R. et al. (1999) J. Virol. 73(4):2886-2892.
2. Zennou, V. et al. (2000) Cell 101(2):173-185.
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