Affinity-tagged protein 정제를 위한 전용 Lysis buffer

다양한 분자량의 affinity-tagged protein을 정제하기 위하여 mammalian cell pellet extraction 뿐만 아니라 bacteria에도 TALON xTractor Buffer를 사용 가능하다. 또한 TALON xTractor Buffer Kit에는 lysozyme과 DNase가 포함되어 있어 bacteria 세포벽과 막을 완전히 파괴할 수 있기 때문에 높은 분자량의 단백질을 효율적으로 추출 가능 하다. 본 제품은 inclusion body에서 불용해성 단백질을 추출하기 위해 사용할 수 있다.

Protein Extraction with xTractor Buffer than Sonication

Extraction
Method

Fraction

Protein
(mg)

Activity

Results

Sonication

Total lysate

28.6

872

Sonication provides lower yield & activity

Purified protein

0.6

2,115

xTractor Buffer Kit

Total lysate

122.1

2,277

xTractor provides higher yield & activity

Purified protein

0.9

3,054

For this comparison of protein extraction methods, LacZ protein was extracted from a 1.7 g pellet of E. coli cells expressing his-tagged protein. Lysates were purified on a TALON column and eluted with 150 nM imidazole. Protein activity was determined by measuring luminescence units.

Figure 1. xTractor Cell Lysis Buffer simplifies protein extraction. After extraction, you can purify your protein with either a standard TALON Resin column after centrifugation or filtration (left), or with the TALON CellThru Column (right).

xTractor Cell Lysis Buffer는 his-tagged proteins 추출에 최적화 되어 있기 때문에, TALON Co Resin 이나 His60 Ni Superflow Resin 또는 다른 종류의 affinity resin에도 사용 할 수 있다.

모든 종류의 단백질 정제시 ProteoGuard Protease Inhibitor Cocktail 과 함께 사용할 것을 추천한다.
Components & Storage Conditions
각 제품 구성물과 보관조건은 Certificate of Analysis 를 참조하십시오.
주요 샘플에 따른 xTractor Buffer Lysis Protocol

적용샘플 종

Protocol (클릭하여 다운로드하세요)

xTractor Buffer 표준 매뉴얼

xTractor Buffer and xTractor Buffer Kit User Manual 

Bacteria

xTractor Buffer Bacterial Sample Preparation Protocol-At-A-Glance 

Baculovirus

xTractor Buffer Baculovirus Sample Preparation Protocol-At-A-Glance 

Mammalian

xTractor Buffer Mammalian Sample Preparation Protocol-At-A-Glance 

Yeast

xTractor Buffer Yeast Sample Preparation Protocol-At-A-Glance