miRNA °°Àº small RNA´Â ¼¼Æ÷ ³» ´ë»ç, ½ÅÈ£Àü´Þ, Áúº´ÀÇ ¿øÀÎÀ» ã±â À§ÇØ ´Ù¾çÇÑ ¿¬±¸¸¦ ÁøÇàµÇ°í ÀÖ½À´Ï´Ù.
´ÙÄ«¶ó´Â miRNA ¹ßÇö ºÐ¼®À» À§ÇÑ µÎ°¡Áö È¿À²ÀûÀÎ ¼Ö·ç¼ÇÀ» Á¦¾ÈÇÕ´Ï´Ù.
💡¼Ö·ç¼Ç1. MicroRNA qRT-PCR Á¤·®
Simple & single-step | ▪Poly(A) polymerase, SMART MMLV RTaseÀÇ ÃÖÀûÈµÈ Á¶ÇÕ ▪¸Å¿ì °£´ÜÇÑ single stepÀ¸·Î miRNAÀÇ cDNA ÇÕ¼º ¿Ï·á |
°í°¨µµ °ËÃâ | ▪TB Green¢â Advantage qPCR Premix·Î °í°¨µµ °ËÃâ : 50 copies ¼öÁرîÁö °ËÃâ °¡´É |
2 in 1 & Flexible use | ▪miRNA Á¤·®¿¡ ÇÊ¿äÇÑ cDNA ÇÕ¼º ½Ã¾à ¹× qPCR premix ¸ðµÎ Æ÷ÇÔ ▪ÇÕ¼ºµÈ cDNA´Â miRNA¿Í mRNA ºÐ¼®¿¡ ¸ðµÎ ÀÌ¿ë °¡´É |
👉 Mir-X¢â miRNA qRT-PCR TB Green¢ç KitÀÇ °£´ÜÇÑ ½ÇÇè°úÁ¤
Step1: 1st Strand cDNA ÇÕ¼º: miRNA 3 ¸»´Ü tailing°ú cDNA ÇÕ¼ºÀ» µ¿½Ã ÁøÇà
Step2: qPCR Á¤·®: miRNA forward primer¿Í Á¦Ç°¿¡ Æ÷ÇÔµÈ mRQ 3 Primer·Î °í°¨µµ Á¤·®
Á¦Ç° ÄÚµå | Á¦Ç°¸í | Ư¡ |
638313 ¿Ü | RNA½Ã·á°¡ Æ÷ÇÔµÈ microRNA·Î °£ÆíÇÏ°Ô ´ÜÀÏ ¹ÝÀÀÀ¸·Î cDNA ÇÕ¼º | |
638314 ¿Ü | °£ÆíÇÑ ´ÜÀÏ ¹ÝÀÀ ´Ü°èÀÇ cDNA ÇÕ¼º |
💡¼Ö·ç¼Ç2. smRNA-Seq (NGS ºÐ¼®)
Simple & single-step | ▪3½Ã°£ À̳»¿¡ single-tube¿¡¼ ½ÇÇè, °£´ÜÇÑ ÇÁ·ÎÅäÄÝ |
°í°¨µµ °ËÃâ | ▪¼Ò·®ÀÇ small RNAs (low-input amounts)ºÎÅÍ °íÇ°ÁúÀÇ Illumina¢ç-ready sequencing library¸¦ Á¦ÀÛ |
³ôÀº ÀçÇö¼º | ▪Adapter ligation free ¹æ½ÄÀ» ÀÌ¿ëÇÏ¿© Ÿ»ç´ëºñ bias ÃÖ¼ÒÈ |
👉 SMARTer¢ç smRNA-Seq Kit for Illumina¢çÀÇ °£´ÜÇÑ ½ÇÇè°úÁ¤
Á¦Ç° ÄÚµå | Á¦Ç°¸í | Ư¡ |
635029 ¿Ü | Small RNA (miRNA, siRNA, piRNA, sno RNA µî) ºÐ¼®À» À§ÇÑ NGS Library Á¦ÀÛ |
Á¦Ç° ¹®ÀÇ»çÇ×Àº °í°´Áö¿ø (02-2081-2510, support@takara.co.kr) ·Î ¿¬¶ô ¹Ù¶ø´Ï´Ù.
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