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event
 Á¦¸ñ : [À̺¥Æ® Á¾·á] [Ä·ÆäÀÎ] ½ÇÇè ÆíÀǼºÀÇ Àý´ë°­ÀÚ EcoDry ½Ã¸®Áî (cDNA ÇÕ¼º, PCR, cloning) 20% ÇÒÀÎ Áß (~6/30)
ÀÛ¼ºÀÏ : 2023.04.28
 ÀÛ¼ºÀÚ : ´ÙÄ«¶óÄÚ¸®¾Æ

EcoDry ½Ã¸®Áî½ÇÇèÀÌ °£´ÜÇÒ¼ö·Ï Error°¡ ÁÙ¾îµì´Ï´Ù.

EcoDry¢â´Â È¿¼Ò, buffer µî ½ÇÇè¿¡ ÇÊ¿äÇÑ ¸ðµç ½Ã¾àÀÌ 1ȸºÐ¾¿ ÇÑ Æ©ºê¿¡ ºÐÁÖ µÇ¾î µ¿°á°ÇÁ¶ ÇüÅ·ΠpremixµÈ Á¦Ç°ÀÔ´Ï´Ù.

µû¶ó¼­, ±âÁ¸ÀÇ ½ÇÇè½Ã°£°ú °úÁ¤À» ´ëÆø ÁÙÀÌ°í ½ÇÇè Æí¸®¼ºÀ» ±Ø´ëÈ­ÇÒ ¼ö ÀÖ½À´Ï´Ù.

ƯÈ÷ ½ÇÇèÀ» óÀ½ Á¢Çϰųª, ±âÁ¸ ½ÇÇè È¿À²ÀÌ ³·¾Ò´ø ¿¬±¸Àڵ鵵 ¼Õ½±°Ô ÁøÇàÇÏ¿© ÀçÇö¼º ³ôÀº ½ÇÇè °á°ú¸¦ ¾òÀ¸½Ç ¼ö ÀÖ½À´Ï´Ù.

ÇöÀç 20% ÇÒÀÎ Ä·ÆäÀÎÀÌ ÁøÇàÁßÀÎ ¸¸Å­ ¸¹Àº °ü½É ºÎŹµå¸³´Ï´Ù.

 

1. RNA to cDNA EcoDry Premix – cDNA ÇÕ¼ºÀ» ÃÖ°í·Î Æí¸®ÇÏ°Ô

Ư¡. 1st strand cDNA ÇÕ¼º¿¡ ÇÊ¿äÇÑ ¸ðµç ½Ã¾àÀÌ Æ÷ÇÔµÈ ready to use Á¦Ç°
- Tube
¿¡ ¹°°ú RNA¸¸ ³ÖÀ¸¸é ¹Ù·Î 1st strand cDNA ÇÕ¼º °¡´É
- 10 kb
ÀÌ»óÀÇ 1st strand cDNA ÇÕ¼º °¡´É, ½ÇÇè ¼±È£µµ¿¡ µû¶ó primer ¼±Åà °¡´É (Oligo dT, Random hexamer, Doubled primed)

Á¦Ç°¸í

Code

Ư¡

RNA to cDNA EcoDry Premix (Oligo dT)

639541

- mRNA 3¡¯ ¸»´Ü (poly A)·ÎºÎÅÍ cDNA ÇÕ¼º

RNA to cDNA EcoDry Premix (Random hexamers)

639544

- Total RNA·ÎºÎÅÍ cDNA ÇÕ¼º

RNA to cDNA EcoDry Premix (Double primed)

639547

- Oligo dT, Random hexamers¸¦ ÇÔ²² »ç¿ë

 

2. High Fidelity PCR EcoDry Premix / High Yield PCR EcoDry¢â Premix – ÃÖ°íÀÇ ÆíÀǼº PCR È¿¼Ò 2Á¾

Ư¡. PCR¿¡ ÇÊ¿äÇÑ ¸ðµç ½Ã¾àÀÌ Æ÷ÇÔµÈ ready to use Á¦Ç°
- Tube
¿¡ template°ú primer, ¹°¸¸ ³ÖÀ¸¸é ¹Ù·Î PCR °¡´É
-
¸ñÀû¿¡ µû¶ó PCRÈ¿¼Ò ¼±Åà °¡´É (High fidelity or High yield)

Á¦Ç°¸í

Code

Ư¡

High Fidelity PCR EcoDry Premix

639280

- Advantage 2 DNA Polymerase ±â¹Ý

- º¸´Ù ³ôÀº Á¤È®µµ·Î ±ä template ÁõÆø °¡´É
- cDNA library, cloning, long PCR
¿¡ ÀûÇÕ

High Yield PCR EcoDry¢â Premix

639544

- TITANIUM Taq DNA Polymerase ±â¹Ý
- Routine PCR (colony PCR, genotyping)
¿¡ ÀûÇÕ

 

3. In-Fusion¢ç Snap Assembly EcoDry¢â Master Mix – In-Fusion¢ç cloningÀ» ÃÖ°í·Î Æí¸®ÇÏ°Ô

Ư¡. In-Fusion¢ç cloning¿¡ ÇÊ¿äÇÑ ¸ðµç ½Ã¾àÀÌ Æ÷ÇÔµÈ ready to use Á¦Ç°
- Tube
¿¡ vector¿Í insert¸¸ ³ÖÀ¸¸é ¹Ù·Î In-Fusion¢ç ¹ÝÀÀ °¡´É
- In-Fusion¢ç Cloning mechanism µ¿¿µ»ó º¸·¯°¡±â
- High-throughput
½ÇÇè¿¡ ÃÖÀû

Á¦Ç°¸í

Code

In-Fusion¢ç Snap Assembly EcoDry¢â Master Mix

638954

In-Fusion¢ç Snap Assembly EcoDry¢â Master Mix (96 well plate)

639544

   ​  

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