EcoDry ½Ã¸®Áî, ½ÇÇèÀÌ °£´ÜÇÒ¼ö·Ï Error°¡ ÁÙ¾îµì´Ï´Ù.
EcoDry¢â´Â È¿¼Ò, buffer µî ½ÇÇè¿¡ ÇÊ¿äÇÑ ¸ðµç ½Ã¾àÀÌ 1ȸºÐ¾¿ ÇÑ Æ©ºê¿¡ ºÐÁÖ µÇ¾î µ¿°á°ÇÁ¶ ÇüÅ·ΠpremixµÈ Á¦Ç°ÀÔ´Ï´Ù.
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ÇöÀç 20% ÇÒÀÎ Ä·ÆäÀÎÀÌ ÁøÇàÁßÀÎ ¸¸Å ¸¹Àº °ü½É ºÎŹµå¸³´Ï´Ù.
1. RNA to cDNA EcoDry Premix – cDNA ÇÕ¼ºÀ» ÃÖ°í·Î Æí¸®ÇÏ°Ô
Ư¡. 1st strand cDNA ÇÕ¼º¿¡ ÇÊ¿äÇÑ ¸ðµç
½Ã¾àÀÌ Æ÷ÇÔµÈ ready to use Á¦Ç°
- Tube¿¡ ¹°°ú RNA¸¸ ³ÖÀ¸¸é ¹Ù·Î 1st
strand cDNA ÇÕ¼º °¡´É
- 10 kb ÀÌ»óÀÇ 1st strand cDNA ÇÕ¼º °¡´É, ½ÇÇè ¼±È£µµ¿¡ µû¶ó primer ¼±Åà °¡´É (Oligo dT, Random hexamer, Doubled primed)
Á¦Ç°¸í |
Code |
Ư¡ |
RNA to
cDNA EcoDry Premix (Oligo dT) |
639541 |
- mRNA 3¡¯ ¸»´Ü (poly A)·ÎºÎÅÍ cDNA ÇÕ¼º |
RNA to
cDNA EcoDry Premix (Random hexamers) |
639544 |
- Total RNA·ÎºÎÅÍ cDNA ÇÕ¼º |
RNA to
cDNA EcoDry Premix (Double primed) |
639547 |
- Oligo dT, Random
hexamers¸¦
ÇÔ²² »ç¿ë |
2. High
Fidelity PCR EcoDry Premix / High
Yield PCR EcoDry¢â Premix – ÃÖ°íÀÇ ÆíÀǼº PCR È¿¼Ò 2Á¾
Ư¡. PCR¿¡ ÇÊ¿äÇÑ ¸ðµç ½Ã¾àÀÌ Æ÷ÇÔµÈ ready to use Á¦Ç°
- Tube¿¡ template°ú primer, ¹°¸¸
³ÖÀ¸¸é ¹Ù·Î PCR °¡´É
- ¸ñÀû¿¡ µû¶ó PCRÈ¿¼Ò ¼±Åà °¡´É (High
fidelity or High yield)
Á¦Ç°¸í |
Code |
Ư¡ |
High
Fidelity PCR EcoDry Premix |
639280 |
- Advantage
2 DNA Polymerase ±â¹Ý - º¸´Ù ³ôÀº Á¤È®µµ·Î ±ä template ÁõÆø °¡´É |
High Yield PCR EcoDry¢â Premix |
639544 |
- TITANIUM
Taq DNA Polymerase ±â¹Ý |
3. In-Fusion¢ç
Snap Assembly EcoDry¢â Master Mix – In-Fusion¢ç
cloningÀ» ÃÖ°í·Î Æí¸®ÇÏ°Ô
Ư¡. In-Fusion¢ç cloning¿¡
ÇÊ¿äÇÑ ¸ðµç ½Ã¾àÀÌ Æ÷ÇÔµÈ ready to use Á¦Ç°
- Tube¿¡ vector¿Í insert¸¸ ³ÖÀ¸¸é
¹Ù·Î In-Fusion¢ç ¹ÝÀÀ °¡´É
- In-Fusion¢ç
Cloning mechanism µ¿¿µ»ó º¸·¯°¡±â
- High-throughput ½ÇÇè¿¡ ÃÖÀû
Á¦Ç°¸í |
Code |
In-Fusion¢ç
Snap Assembly EcoDry¢â Master Mix |
638954 |
In-Fusion¢ç
Snap Assembly EcoDry¢â Master Mix (96 well plate) |
639544 |