Nuclease의 특성 비교
Nuclease의 특성 비교
| S1 Nuclease | Mung Bean Nuclease | BAL31 Nuclease | Exonuclease I | Exonuclease III | |
|---|---|---|---|---|---|
| 반응 특이성 | Endonuclease ssDNA, ssRNA |
Endonuclease ssDNA, ssRNA |
Endonuclease ssDNA, ssRNA Exonuclease 5’→ 3’, 3’→ 5’ssDNA |
Exonuclease 5’→ 3’, 3’→ 5’ssDNA | |
| 최종 생성물 | 5’-P mononucleotide 5’-P oligonucleotide |
5’-P mononucleotide 5’-P oligonucleotide |
5’-P mononucleotide | 5’-P mononucleotide | ssDNA 5’-P mononucleotide |
| 분자량 | 32,000 | 39,000 | 73,000~83,000 | 52,000 | 28,000 |
| 최적 pH | 4.5 | 5.0 | 8.1 | 9.5 | 8.0 |
| 금속이온 | Zn2＋ | Zn2＋ | Ca2＋ , Mg2＋ |
Mg2＋ | Mg2＋ |
| Deoxyribonuclease I | Ribonuclease H | Micrococcal Nuclease | ECloned DNase I | |
|---|---|---|---|---|
| 반응 특이성 | Endonuclease ssDNA, dsDNA |
RNA-DNA Hybrid 특이적 Endonuclease |
Endonuclease ssDNA, ssRNA ssRNA |
Exonuclease ssDNA, dsDNA |
| 최종 생성물 | 5’-P mononucleotide | ssDNA 5’-P mononucleotide |
3’-P mononucleotide 3’-P oligonucleotide |
5’-P oligonucleotide |
| 분자량 | 31,000 | 21,000 | 16,800 | 30,000 |
| 최적 pH | 7.0 | 8.0 | 9.2 | 7.0 |
| 금속이온 | Mg2＋, Mn2＋ | Mg2＋, Mn2＋ | Ca2＋ | Ca2＋, Mg2＋, Mn2＋ |