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Inducible System ¡¤ Yeast hybrid
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Yeast Two Hybrid System (Y2H)
> Y2H Library Construction System
Y2H Library Construction System
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Clontech
630490
Make Your Own ¡°Mate & Plate¡± Library System
5 ȸ
(Package)
3,120,000¿ø
,
,
Product index
Tet À¯µµ ¹ßÇö ½Ã½ºÅÛ
[°³¿ä] Tet-on system
[¼±Åð¡À̵å] Tet Syste..
Tet-On 3G À¯µµ ¹ßÇö ½Ã..
Lenti-X Tet-On 3G Indu..
Tet-On 3G Cell Line
Tet Approved FBS
Tet °ü·Ã Ç×»ýÁ¦
Selection marker (Line..
Tet-One Inducible Expr..
TetR Ç×ü
[Legacy] Tetracycline ..
Tet-Off Cell Lines
pTRE-Tight Vectors
pTet-tTS Vectors
IRES Tet À¯µµ¹ßÇö ½Ã½º..
iDimerize Inducible System..
[°³¿ä] Inducible Prote..
iDimerize Antibodies
Inducible Expression S..
Heterodimer System
Homodimer System
Regulated Transcriptio..
Reverse Dimerization S..
´Ü¹éÁú ¹ßÇö·® Á¶Àý ½Ã½ºÅÛ ..
ProteoTuner System
Tet-ProteoTuner Vector..
[°³¿ä] Yeast Hybrid System
[FAQ] Matchmaker Gold ..
[°³¿ä] Matchmaker Gold..
Yeast Two Hybrid System (Y..
Y2H Library Constructi..
Yeast Culture media (T..
Yeast Two-Hybrid (Y2H)..
Y2H Library (Yeast ³»..
Normalized Y2H Library..
Yeast Two hybrid vecto..
pBridge Vector
Yeast One-Hybrid (Y1H)
Yeast Culture Media (O..
Yeast One-Hybrid (Y1H)..
Matchmaker °ü·ÃÁ¦Ç°
Yeast Culture Media
Yeast Culture Media (..
Aureobasidin A
Yeast Plasmid Isolati..
Matchmaker Ç×ü
Insert Check Kit (prey..
Yeast Transformation M..
X-¥á-Gal
Yeast Transformation S..
Mammalian Cell Assay K..
SMART ±â¼úÀ» »ç¿ëÇÏ¿© yeast¿¡¼ Á÷Á¢ library Á¦ÀÛ
¾î·Á¿î cloningÀ̳ª library ÁõÆø °úÁ¤ ºÒÇÊ¿ä
Yeast two-hybrid screeningÀ» ¼ö¹éȸ ÇÒ ¼ö ÀÖÀ» ¸¸Å ÃæºÐÇÑ ½Ã¾àÀ¸·Î ±¸¼ºµÊ
Mate & Plate ÀåÁ¡
ÀüÇüÀûÀÎ ¹æ¹ýÀ¸·Î yeast two-hybrid¿ë library¸¦ Á¦ÀÛÇÏ°í screening ÇÏ´Â °ÍÀº ³ëµ¿ Áý¾àÀûÀÎ °úÁ¤ÀÌ´Ù. ÇÏÁö¸¸, Mate & Plate Library´Â ¹Ì¸® library·Î ÇüÁú ÀüȯµÈ yeast strain (MAT¥á)À¸·Î »õ bait ´Ü¹éÁúÀ» ¹ßÇöÇÏ´Â reporter strain (MAT¥á)°ú ÇÔ²² ÇÏ·í¹ã ¹è¾çÇÏ¿© ÀûÀýÇÑ selective/minimal ¹èÁö¿¡ µµ¸»ÇÏ¸é µÇ¹Ç·Î, ¾ÆÁÖ ½±°Ô »óÈ£ÀÛ¿ëÀ» Ž»öÇÒ ¼ö ÀÖ´Ù. ClontechÀº library screening¿¡ Àû¿ë °¡´ÉÇÑ ´Ù¾çÇÑ ÇüÅÂÀÇ tissue-specific library, normalized library ¹× universal library¸¦ Á¦°øÇÏ°í ÀÖ´Ù.
Figure 1. Library generation using in vivo homologous recombination in yeast.
Mate & Plate Libraries are created via recombination between your SMART-generated cDNA and the Matchmaker prey vector, pGADT7-Rec. Transformed yeast colonies are pooled, mixed, and aliquoted into multiplevials. Each single 1 ml vial can be used for a two-hybrid screen.
°£´ÜÇÏ°í ºü¸£°Ô ¸¸µé ¼ö ÀÖ´Â ÀڽŸ¸ÀÇ Library
ClontechÀÇ Mate & Plate Library¿¡¼ ÀÚ½ÅÀÇ ¸ñÀû¿¡ ÀûÇÕÇÑ Library¸¦ ãÀ» ¼ö ¾ø´Ù¸é, Make Your Own Mate & Plate Library SystemÀ» »ç¿ëÇÏ´Â °Íµµ ÁÁ´Ù. º» systemÀ» »ç¿ëÇÏ¸é ¼ö ¹éȸÀÇ yeast two-hybrid screeningÀ» ÇÒ ¼ö ÀÖÀ» ¸¸Å ÃæºÐÇÑ ¾çÀÇ library¸¦ ÀÏÁÖÀÏ ¾È¿¡ Á¦ÀÛÇÒ ¼ö ÀÖ´Ù. Library Á¦ÀÛÀº yeast ÀÚüÀÇ homologous recombination Çö»óÀ» »ç¿ëÇÏ¿© Y187 library yeast strain¿¡¼ Á÷Á¢ ÀÌ·ç¾îÁø´Ù (Figure 1). ÀϹÝÀûÀÎ library Á¦ÀÛ °úÁ¤¿¡ ÇÊ¿äÇÑ ¼ö°í (¿¹: library cloning, ÁõÆø, Á¤Á¦ µî)¸¦ ÇÇÇÒ ¼ö ÀÖ´Ù.
°æÁ¦ÀûÀÎ SMART cDNA ÇÕ¼º ±â¼ú
º» systemÀº ClontechÀÇ SMART cDNA ÇÕ¼º ±â¼úÀ» »ç¿ëÇÏ¿© total RNA 100 ng Á¤µµÀÇ ÀûÀº ¾çÀ¸·Î ¾î¶°ÇÑ Á¶Á÷ÀÇ cDNA libraryµµ Á¦ÀÛÇÒ ¼ö ÀÖ´Ù. SMART ±â¼úÀº Moloney murine leukemia virus ¿ªÀü»çÈ¿¼ÒÀÇ terminal transferase¿Í template switching activity¸¦ ÀÌ¿ëÇÏ¿© ù ¹ø°·Î ÇÕ¼ºµÇ´Â cDNA ¾ç ¸»´Ü¿¡ ¼¿À» ¾Ë°í ÀÖ´Â primer binding site°¡ »ý¼ºµÇµµ·Ï ÇÑ´Ù.µû¶ó¼ SMART ±â¼ú·Î ÇÕ¼ºµÈ cDNA´Â ¹Ù·Î PCR ÁÖÇüÀ¸·Î »ç¿ëÀÌ °¡´ÉÇÏ´Ù. ¶ÇÇÑ, ÀÌ·¸°Ô ÁõÆøµÈ cDNA´Â homologous recombinationÀ» ÀÌ¿ëÇÏ¿© Matchmaker Gold prey plasmidÀÎ pGADT7-RecÀ¸·Î cloningÇÒ ¼ö ÀÖ´Ù. ÀÌ·¯ÇÑ Æ¯¼ºÀ¸·Î ÀÎÇÏ¿© ng ¼öÁØÀÇ RNA (microdissected tissues, lasercaptured cells, biopsy samples µî)·ÎºÎÅÍ cDNA¸¦ ÇÕ¼ºÇÏ¿© pGADT7-Rec vector¿Í ÇÔ²² yeast strain Y187·Î co-transformationÇÏ¿© Á÷Á¢ÀûÀ¸·Î library¸¦ Á¦ÀÛÇÒ ¼ö ÀÖ´Ù.
Components & Storage Conditions
°¢ Á¦Ç° ±¸¼º¹°°ú º¸°üÁ¶°ÇÀº Certificate of Analysis ¸¦ ÂüÁ¶ÇϽʽÿÀ.
Keyword :
Yeast Two Hybrid System (Y2H)
Y2H Library Construction System
Yeast Culture media (Two-Hybrid ¿ë)
Yeast Two-Hybrid (Y2H) System
Y2H Library (Yeast ³»¿¡ µµÀÔµÈ »óÅÂ)
Normalized Y2H Library (Yeast ³»¿¡ µµÀÔµÈ »óÅÂ)
Yeast Two hybrid vectors
pBridge Vector