제품설명

Ingenio^® Electroporation Kit는 핵산도입이 어려운 mammalian cell, 특히 primary cell에 대하여 합리적인 비용으로 높은 효율의 핵산도입과 저독성을 실현한 electroporation전용 시약이다. 본 제품은 Electroporation solution, cuvettes, Cell Dropers로 구성되어 있고, Bio-Rad, Lonza, BTX, Eppendolf 등의 electroporation 장비에 최적의 효율을 가지도록 고안되었기 때문에 별도의 전용장비를 구매할 필요가 없다.
Ingenio^® 0.2㎝ cuvettes는 Amaxa^® Nucleofector^® II/2b Device에 적합하고, Ingenio^® 0.4 ㎝ cuvettes는 Bio-Rad Gene Pulser Xcell™ Electroporator에 적합하다.
- Ingenio^® Pulse Condition Recommendations (pdf)
- Electroporating Primary cells and Other Hard to Transfect Cell Types using Ingenio^® Electroporation Solution (pdf)
- Electroporation of Human Induced Pluripotent Stem (iPS) Cells with Ingenio^® Electroporation Solution (pdf)

내용

Ingenio^® Electroporation Kits with cuvettes

Code	구성품			적용가능한 기기
Code	Ingenio^® Solution	Cuvettes	Cell Droppers	적용가능한 기기
MIR 50112	6.25 ㎖	0.2 ㎝cuvettes × 25개	25 개	- Lonza Amaxa^® Nucleofector^® II/2b Device
MIR 50115	12.5 ㎖	0.2 ㎝cuvettes × 50개	50 개
MIR 50118	12.5 ㎖×2	0.2 ㎝cuvettes × 100개	100 개
MIR 50113	6.25 ㎖	0.4 ㎝cuvettes × 25개	25 개	- Bio-Rad Gene Pulser Xcell™ Electroporator - Harvard-BTX
MIR 50116	12.5 ㎖	0.4 ㎝cuvettes × 50개	50 개
MIR 50119	12.5 ㎖×2	0.4 ㎝cuvettes × 100개	100 개

Ingenio^® Electroporation Solution

Code	용량
MIR 50111	6.25 ㎖
MIR 50114	12.5 ㎖
MIR 50117	12.5 ㎖×2

보존

Ingenio^® Solution : 4℃
Ingenio^® Cuvettes : 실온
Ingenio^® Cell Dropers : 실온

실험 예

1. Lonza Amaxa^® Nucleofector^® II/2b Device에 적용예

Ingenio^® Electroporation Kits were used to transfect indicated cell types using Amaxa^® Nucleofector^® II Device. Cells were assayed at 24 hours by flow cytometry and reported as percentage of live cell population.
*Primary cell type

2. Bio-Rad Gene Pulser Xcell™ Electroporator에 적용예

Cells were electroporated with an EGFP reporter vector using the Ingenio^® Electroporation Solution and a Gene Pulser Xcell™ Eukaryotic System. EGFP expressing cells were identified 24 hours post-electroporation by flow cytometry and presented as a percentage of the live cell population.

제품설명

내용

보존

실험 예

관련제품