¿À´Ã ÇÏ·ç ±×¸¸º¸±â
´Ý±â
·Î±×ÀÎ
|
ȸ¿ø°¡ÀÔ
|
ID/PW ã±â
|
Á¦Ç°»ó´ã¹®ÀÇ
°í°´Áö¿ø
¾÷¹«¾È³»¦¢Á¦Ç°¹®ÀÇ
ÀüȹøÈ£¦¢
02-2081-2510
Email ¦¢
support@takara.co.kr
´ëÀüÁö»ç
¾÷¹«¾È³»¦¢´ëÀü/ÃæûÁö¿ª ÁÖ¹®, Á¦Ç°¹®ÀÇ
ÀüȹøÈ£¦¢
042-828-6525
Email ¦¢
tkbd@takara.co.kr
¾÷¹«½Ã°£¾È³»
[ Æò¡¡¡¡ÀÏ ] 09 : 00 ~ 18 : 00 ¦¢ [ Á¡½É½Ã°£ ] 12 : 00 ~ 13 : 00
Å䡤ÀÏ¿äÀÏ, °øÈÞÀÏÀº ÈÞ¹«ÀÔ´Ï´Ù.
Á¦Ç°Á¤º¸
°øÁö
±â¼úÁö¿ø
Á¤º¸ÀÚ·á
Learning Center
ȸ»çÁ¤º¸
ÀüÁ¦Ç°º¸±â
Á¦Ç°¼±Åð¡À̵å
Custom Service
À̺¥Æ®/ÇÁ·Î¸ð¼Ç
´ÙÄ«¶ó¼Ò½Ä
Takara Bio°ø½Ã
½ÅÁ¦Ç°¾È³»
¶óÀ̼±½º¾È³»
±â¼úÁö¿ø¹®ÀÇ
±â±âµ¥¸ð½Åû
¼¼¹Ì³ª½Åû
LMO¾È³»
ºê·Î¼Å
On-line tool
eNews
樨毢
CoA & MSDS
°³¿ä & ¸®½ºÆ®
¼Ò°³
µµÀԺ귣µå Á¤º¸
´ë¸®Á¡Á¤º¸
ã¾Æ¿À½Ã´Â±æ
Home
>
ÀüÁ¦Ç°º¸±â
>
Transfection ½Ã¾à
>
´Ù¾çÇÑ ¼¼Æ÷/¹ü¿ëÀûÀÎ µµÀԽþà
>
Trans
IT-X2¢ç Transfection Reagent
¹ü¿ë¼º No.1, Plasmid DNA¿Í siRNA/miRNA ¸ðµÎ Àû¿ë
Trans
IT-X2¢ç Transfection Reagent
Á¦Á¶»ç
Á¦Ç°ÄÚµå
Á¦Ç°¸í
¿ë·®
°¡°Ý
(ºÎ°¡¼¼º°µµ)
ºñ°í
»ç¿ëÀڸŴº¾ó
Mirus
MIR 6003
Trans
IT-X2 Dynamic Delivery System
0.3 ml
°¡°Ý¹®ÀÇ
,
Mirus
MIR 6004
Trans
IT-X2 Dynamic Delivery System
0.75 ml
°¡°Ý¹®ÀÇ
Mirus
MIR 6000
Trans
IT-X2 Dynamic Delivery System
1.5 ml
°¡°Ý¹®ÀÇ
Mirus
MIR 6005
Trans
IT-X2 Dynamic Delivery System
1.5 ml¡¿5
°¡°Ý¹®ÀÇ
Mirus
MIR 6006
Trans
IT-X2 Dynamic Delivery System
1.5 ml¡¿10
°¡°Ý¹®ÀÇ
Product index
Overview
[FAQ] Mirus transfecti..
Cell lineº° ¼±Åð¡À̵å
PACKAGE DELIVERY: The ..
´Ù¾çÇÑ ¼¼Æ÷/¹ü¿ëÀûÀÎ µµÀÔ..
TransIT-X2¢ç Transfect..
TransIT¢ç-LT1 Transfec..
TransIT¢ç-2020 Transfe..
Xfect¢â Transfection R..
CalPhos¢â Mammalian Tr..
È¿À²Áõ´ë¸¦ À§ÇÑ Æ¯¼öµµÀÔ½Ã..
TransIT-VirusGEN¢ç Tra..
TransIT¢ç-Lenti Transf..
TransIT-PRO¢ç Transfec..
RevIT¢â AAV Enhancer
VirusGEN¢ç AAV Transfe..
VirusGEN¢ç LV Transfec..
¼¼Æ÷¿¡ µû¸¥ È¿À²ÀûÀÎ À¯Àü..
TransIT¢ç-293 Transfec..
TransIT¢ç-BrCa Transfe..
TransIT¢ç-CHO Transfec..
TransIT-HeLaMONSTER¢ç ..
TransIT¢ç-Jurkat Trans..
TransIT¢ç-Keratinocyte..
TransIT¢ç-Insect Trans..
Xfect mESC Transfectio..
Electroporation °ü·Ã
Ingenio¢ç solution & k..
Ingenio¢ç cuvette, ¼Ò..
µµÀÔ¹°Áú¿¡ µû¸¥ µµÀԽþà
TransIT¢ç-mRNA Transfe..
TransIT¢ç-Oligo Transf..
Xfect¢â RNA Transfecti..
Xfect¢â Protein Transf..
in-vivo delivery ½Ã½ºÅÛ
TransIT Hydrodynamic D..
µ¶¼º Á¦°Å - Transfection ..
MiraCLEAN¢ç Endotoxin ..
siRNA Transfection ½Ã¾à
Label IT Delivery Cont..
Label IT siRNA Tracker..
TransIT-QR Hydrodynami..
TransIT-siQUEST Transf..
TransIT-TKO Transfecti..
TransIT-X2 Dynamic Del..
siRNA ¹ßÇö ¡¤ ºÐ¼®
pSINsi vector ½Ã¸®Áî
Synthetic siRNA Quanti..
small RNA Cloning
pBApo-CMV vector ½Ã¸®..
pBApo-EF1¥á Vector ½Ã..
small RNA Cloning Kit
small RNA Gel Extracti..
RNAi °ü·Ã Kit
14-30 ssRNA Ladder Mar..
siRNA Ladder Marker
´Ù¾çÇÑ cell type°ú ´Ù¾çÇÑ application¿¡ °íÈ¿À² Àû¿ë °¡´É (±×¸² 1)
DNA¿Í siRNA/miRNA, co-transfection ¸ðµÎ °¡´É
Primary cellÀ» Æ÷ÇÔÇÑ ´Ù¾çÇÑ ¼¼Æ÷¿¡ È¿À²ÀûÀ¸·Î µµÀÔ:
ÁÖ¿ä cell lineº° ½ÇÇè °¡À̵å¶óÀÎ º¸±â (Ŭ¸¯)
Animal origin Free
CRISPR/Cas9 ½ÇÇè¿¡µµ ÃÖÀû: Cas9-sgRNA Á¶ÇÕ¿¡ µû¸¥ ½ÇÇè °¡À̵å¶óÀÎ Á¦°ø
-
Trans
IT-X2
¢ç
for CRISPR RNP Delivery (
PDF ¸µÅ©
)
-
Trans
IT-X2
¢ç
for CRISPR RNP + ssODN Delivery Protocol (
PDF ¸µÅ©
)
-
Trans
IT-X2
¢ç
for CRISPR pDNA/gRNA Delivery Protocol (
PDF ¸µÅ©
)
-
Trans
IT-X2
¢ç
for CRISPR Plasmid Delivery Protocol (
PDF ¸µÅ©
)
±×¸² 1.
Trans
IT
¢ç
-X2 SystemÀº ´Ù¾çÇÑ cell type¿¡¼ °íÈ¿À²·Î À¯ÀüÀÚ ¹ßÇö °¡´É.
Trans
IT-X2
¢ç
Dynamic Delivery System°ú Lipofectamine
¢ç
2000 Transfection Reagent¸¦ »ç¿ëÇؼ luciferase¸¦ encodingÇÏ´Â plasmid DNA¸¦ 41°³ÀÇ ´Ù¸¥ cell type¿¡ 3 : 1 (reagent : DNA)ÀÇ ºñÀ²·Î transfection ÇÏ¿´´Ù. Transfection 24½Ã°£ ÈÄ¿¡ standard luciferase assay¸¦ ÁøÇàÇÑ °á°ú
Trans
IT-X2
¢ç
¸¦ »ç¿ëÇÏ´Â °æ¿ì 41°³ Áß 36°³ÀÇ cell type¿¡¼ luciferase È°¼ºÀÌ µ¿µîÇϰųª ´õ ³ô¾Ò´Ù. ÀÌ Áß 17°³ÀÇ cell type¿¡¼
Trans
IT-X2
¢ç
°¡ 2¹è ÀÌ»ó ³ô¾Ò°í ÇØ´ç cell typeÀ» ¢Ô·Î Ç¥½ÃÇÏ¿´´Ù.
Á¦Ç° ¼³¸í
TransIT-X2
¢ç
Dynamic Delivery SystemÀº non-liposomal polymeric ½Ã½ºÅÛÀ¸·Î primary cellÀ» Æ÷ÇÔÇÑ ´Ù¾çÇÑ cell type¿¡¼ °íÈ¿À² transfectionÀÌ °¡´ÉÇÏ´Ù. ÀÌ Á¦Ç°À» »ç¿ëÇϸé DNA, siRNA/RNAi ¹× DNA¿Í siRNA µîÀÇ co-transfectionÀ» ÁøÇàÇÒ ¼ö ÀÖ´Ù. ¶ÇÇÑ serum compatible transfectionÀÌ Æ¯Â¡À¸·Î transfection ÈÄ º°µµÀÇ media change°¡ ÇÊ¿äÇÏÁö ¾Ê´Ù´Â ÀåÁ¡ÀÌ ÀÖ´Ù.
º¸Á¸ -20¡É
°ü·ÃÁ¦Ç°
- Á¦ÇÑÈ¿¼Ò site¸¦ °í·ÁÇÏÁö ¾Ê´Â PCR cloing ¿øÁ¶
In-Fusion
¢ç
Snap Assembly Master Mix (Code 638947)
- Mycoplasma ¿À¿° È®ÀÎ ½Ã¾à
: Nested PCR·Î Á¤¹Ð °ËÃâ
TaKaRa PCR Mycoplasma Detection Set
: °í°¨µµÀÇ cycling probe qPCR ÀÌ¿ë
CycleavePCR Mycoplasma Detection Kit
: Luminometer·Î 20ºÐ¸¸¿¡ °ËÃâ
MycoAlert PLUS mycoplasma detection kit
- RNP µµÀÔµµ °¡´ÉÇÑ:
Guide-it¢â Recombinant Cas9 (Electroporation-Ready)
- sgRNA ÇÕ¼º & ½ºÅ©¸®´×:
Guide-it¢â Complete sgRNA Screening System
)
½ÇÇè µ¥ÀÌÅÍ
±×¸²2. Ÿ»çÁ¦Ç° ´ëºñ ³ôÀº transfection È¿À²°ú ³·Àº ¼¼Æ÷µ¶¼ºÀ» ³ªÅ¸³»´Â
Trans
IT-X2
¢ç
Dynamic Delivery System.
A549 (A)¿Í MDCK (B) ¼¼Æ÷¿¡ luciferase¸¦ encodingÇÏ´Â plasmid¸¦
Trans
IT-X2
¢ç
, Lipofectamine
¢ç
2000 (Thermo Fisher Scientific), Lipofectamine
¢ç
3000 (Thermo Fisher Scientific)À¸·Î °¢°¢ÀÇ ÃÖÀû Á¶°Ç¿¡¼ 24½Ã°£ µ¿¾È transfection ÇÑ °á°úÀÌ´Ù. Luciferase activity·Î transfection È¿À²À» ÃøÁ¤ÇÏ°í, ¼Õ»óµÈ ¼¼Æ÷ÀÇ ¼¼Æ÷Áú¿¡¼ ¹æÃâµÇ´Â LDH¸¦ Á¤·®ÇØ ¼¼Æ÷µ¶¼ºÀ» Æò°¡ÇÏ¿´´Ù. ±× °á°ú Lipofectamine
¢ç
2000 ¹× Lipofectamine
¢ç
3000¿¡ ºñÇØ
Trans
IT-X2
¢ç
¿¡¼ ³ôÀº transgene ¹ßÇöÀ²°ú ³·Àº ¼¼Æ÷µ¶¼ºÀ» È®ÀÎÇÒ ¼ö ÀÖ¾ú´Ù.
±×¸² 3. ´Ù¾çÇÑ cell-line ¹× primary cell¿¡¼
°íÈ¿À² GFP ¹ßÇö.
A549, CHO-K1, Hep G2, MDCK, LNCaP, PC-12, primary human mammary epithelial cells (HMEC), normal human dermal fibroblasts (NHDF) ¼¼Æ÷¿¡ EGFP¸¦ encodingÇÏ´Â plasmid¸¦
Trans
IT-X2
¢ç
Dynamic Delivery System·Î transfection ÇÑ °á°úÀÌ´Ù. 96-well plate¿¡¼ 0.2~0.4 §¡ÀÇ
Trans
IT-X2
¢ç
·Î 0.1 §¶ÀÇ DNA¸¦ transfection Çß°í (2:1, 3:1, 4:1 / reagent: DNA ratio), 48½Ã°£ µÚ 3¹Ýº¹À¸·Î guava easyCyte¢â 5HT Flow Cytometer¿¡¼ ºÐ¼®µÇ¾ú´Ù. ±× °á°ú
Trans
IT-X2
¢ç
Dynamic Delivery SystemÀº ´Ù¾çÇÑ cell-line ¹× primary cell¿¡¼ °íÈ¿À²·Î GFP¸¦ ¹ßÇö½Ãų ¼ö ÀÖ¾ú´Ù.
* Primary cell type
±×¸² 4. Ÿ»çÁ¦Ç° ´ëºñ RNPÀÇ transfection È¿À²ÀÌ ³ôÀº
Trans
IT-X2
¢ç
Dynamic Delivery System.
HEK293T/17°ú U2OS ¼¼Æ÷¿¡
2-part gRNA (IDT)¸¦ Ÿ°ÙÇÏ´Â PPIB (cyclophilin B)¿Í Cas9 protein (PNA Bio)À¸·Î Á¦ÀÛÇÑ Ribonucleoprotein (RNP) complexes¸¦
Trans
IT-X2
¢ç
Dynamic Delivery System (1 §¡/well) ¶Ç´Â Lipofectamine
¢ç
CRISPRMAX¢â (1.5 §¡/well and 1 §¡/well of Lipofectamine
¢ç
Cas9 Plus¢â Reagent, ThermoFisher), Lipofectamine
¢ç
RNAiMAX (1.5 §¡/well), Lipofectamine
¢ç
3000 (1.5 ¥ìl/well and 1 ¥ìl/well of P3000¢â Reagent, ThermoFisher)·Î Á¦Á¶»çÀÇ ÇÁ·ÎÅäÄÝ¿¡ µû¶ó transfection ÇÑ °á°úÀÌ´Ù. 6 nM Cas9 protein (PNA Bio)°ú ´Ù¾çÇÑ ¾çÀÇ gRNA (6 nM, 12 nM)·Î Å×½ºÆ®µÇ¾ú°í, transfection 48½Ã°£ ÈÄ T7E1 mismatch detection assay·Î cleavage efficiency¸¦ ÃøÁ¤ÇÏ¿´´Ù. ±× °á°ú
Trans
IT-X2
¢ç
Dynamic Delivery SystemÀº Ÿ»ç ´ëºñ ³ôÀº È¿À²·Î RNP¸¦ µµÀÔÇÏ¿© °íÈ¿À²·Î À¯ÀüÀÚ ÆíÁýÀ» ÁøÇàÇÒ ¼ö ÀÖ¾ú´Ù.
Keyword : v6003,v6004,v6000,v6005,v6006,mir6003,mir6004,mir6000,mir6005,mir6006,CRISPR,Cas9
´Ù¾çÇÑ ¼¼Æ÷/¹ü¿ëÀûÀÎ µµÀԽþà
¹ü¿ë¼º No.1, Plasmid DNA¿Í siRNA/miRNA ¸ðµÎ ..
Trans
IT-X2¢ç Transfection Reagent
Àúµ¶¼º No.1, µ¶¼º¿¡ ¹Î°¨ÇÑ ¼¼Æ÷µµ °íÈ¿À²·Î p..
Trans
IT¢ç-LT1 Transfection Reagent
Animal origin free·Î ¹ü¿ëÀûÀÎ »ç¿ë
Trans
IT¢ç-2020 Transfection Reagent
Mammalian cell ¿ë °íÈ¿À² ¡¤ Àúµ¶¼º Transfect..
Xfect¢â Transfection Reagent
CalPhos¢â Mammalian Transfection Kit