¿À´Ã ÇÏ·ç ±×¸¸º¸±â
´Ý±â
·Î±×ÀÎ
|
ȸ¿ø°¡ÀÔ
|
ID/PW ã±â
|
Á¦Ç°»ó´ã¹®ÀÇ
°í°´Áö¿ø
¾÷¹«¾È³»¦¢Á¦Ç°¹®ÀÇ
ÀüȹøÈ£¦¢
02-2081-2510
Email ¦¢
support@takara.co.kr
´ëÀüÁö»ç
¾÷¹«¾È³»¦¢´ëÀü/ÃæûÁö¿ª ÁÖ¹®, Á¦Ç°¹®ÀÇ
ÀüȹøÈ£¦¢
042-828-6525
Email ¦¢
tkbd@takara.co.kr
¾÷¹«½Ã°£¾È³»
[ Æò¡¡¡¡ÀÏ ] 09 : 00 ~ 18 : 00 ¦¢ [ Á¡½É½Ã°£ ] 12 : 00 ~ 13 : 00
Å䡤ÀÏ¿äÀÏ, °øÈÞÀÏÀº ÈÞ¹«ÀÔ´Ï´Ù.
Á¦Ç°Á¤º¸
°øÁö
±â¼úÁö¿ø
Á¤º¸ÀÚ·á
Learning Center
ȸ»çÁ¤º¸
ÀüÁ¦Ç°º¸±â
Á¦Ç°¼±Åð¡À̵å
Custom Service
À̺¥Æ®/ÇÁ·Î¸ð¼Ç
´ÙÄ«¶ó¼Ò½Ä
Takara Bio°ø½Ã
½ÅÁ¦Ç°¾È³»
¶óÀ̼±½º¾È³»
±â¼úÁö¿ø¹®ÀÇ
±â±âµ¥¸ð½Åû
¼¼¹Ì³ª½Åû
LMO¾È³»
ºê·Î¼Å
On-line tool
eNews
樨毢
CoA & MSDS
°³¿ä & ¸®½ºÆ®
¼Ò°³
µµÀԺ귣µå Á¤º¸
´ë¸®Á¡Á¤º¸
ã¾Æ¿À½Ã´Â±æ
Home
>
ÀüÁ¦Ç°º¸±â
>
Transfection ½Ã¾à
>
´Ù¾çÇÑ ¼¼Æ÷/¹ü¿ëÀûÀÎ µµÀԽþà
> Xfect¢â Transfection Reagent
Mammalian cell ¿ë °íÈ¿À² ¡¤ Àúµ¶¼º Transfection ½Ã¾à
Xfect¢â Transfection Reagent
Á¦Á¶»ç
Á¦Ç°ÄÚµå
Á¦Ç°¸í
¿ë·®
°¡°Ý
(ºÎ°¡¼¼º°µµ)
ºñ°í
»ç¿ëÀڸŴº¾ó
Clontech
631317
Xfect¢â Transfection Reagent
50 ȸ¡¿2
357,000¿ø
,
,
,
,
Clontech
631318
Xfect¢â Transfection Reagent
100 ȸ¡¿3
868,000¿ø
Product index
Overview
[FAQ] Mirus transfecti..
Cell lineº° ¼±Åð¡À̵å
PACKAGE DELIVERY: The ..
´Ù¾çÇÑ ¼¼Æ÷/¹ü¿ëÀûÀÎ µµÀÔ..
TransIT-X2¢ç Transfect..
TransIT¢ç-LT1 Transfec..
TransIT¢ç-2020 Transfe..
Xfect¢â Transfection R..
CalPhos¢â Mammalian Tr..
È¿À²Áõ´ë¸¦ À§ÇÑ Æ¯¼öµµÀÔ½Ã..
TransIT-VirusGEN¢ç Tra..
TransIT¢ç-Lenti Transf..
TransIT-PRO¢ç Transfec..
RevIT¢â AAV Enhancer
VirusGEN¢ç AAV Transfe..
VirusGEN¢ç LV Transfec..
¼¼Æ÷¿¡ µû¸¥ È¿À²ÀûÀÎ À¯Àü..
TransIT¢ç-293 Transfec..
TransIT¢ç-BrCa Transfe..
TransIT¢ç-CHO Transfec..
TransIT-HeLaMONSTER¢ç ..
TransIT¢ç-Jurkat Trans..
TransIT¢ç-Keratinocyte..
TransIT¢ç-Insect Trans..
Xfect mESC Transfectio..
Electroporation °ü·Ã
Ingenio¢ç solution & k..
Ingenio¢ç cuvette, ¼Ò..
µµÀÔ¹°Áú¿¡ µû¸¥ µµÀԽþà
TransIT¢ç-mRNA Transfe..
TransIT¢ç-Oligo Transf..
Xfect¢â RNA Transfecti..
Xfect¢â Protein Transf..
in-vivo delivery ½Ã½ºÅÛ
TransIT Hydrodynamic D..
µ¶¼º Á¦°Å - Transfection ..
MiraCLEAN¢ç Endotoxin ..
siRNA Transfection ½Ã¾à
Label IT Delivery Cont..
Label IT siRNA Tracker..
TransIT-QR Hydrodynami..
TransIT-siQUEST Transf..
TransIT-TKO Transfecti..
TransIT-X2 Dynamic Del..
siRNA ¹ßÇö ¡¤ ºÐ¼®
pSINsi vector ½Ã¸®Áî
Synthetic siRNA Quanti..
small RNA Cloning
pBApo-CMV vector ½Ã¸®..
pBApo-EF1¥á Vector ½Ã..
small RNA Cloning Kit
small RNA Gel Extracti..
RNAi °ü·Ã Kit
14-30 ssRNA Ladder Mar..
siRNA Ladder Marker
³ôÀº ¼öÁØÀÇ À¯ÀüÀÚ ¹ßÇö °¡´É
³·Àº µ¶¼ºÀÇ »ýºÐÇؼº ³ª³ëÀÔÀÚ (biodegradable nonoparticles)
Ç÷û À¯¹«¿¡ »ó°ü¾øÀÌ »ç¿ë °¡´ÉÇÑ °£ÆíÇÑ Á¶ÀÛ °úÁ¤
Xfect´Â 2,300 Á¾·ù ÀÌ»óÀÇ polymer¸¦ ¼±º°ÇÏ¿© ¾ö¼±ÇÑ °íÈ¿À²ÀÇ transfection ½Ã¾àÀ̸ç, DNA°¡ °áÇÕÇÏ¿© »ýºÐÇؼº, ºñµ¶¼ºÀÇ ³ª³ëÀÔÀÚ¸¦ Çü¼ºÇÏ¿© DNA¸¦ È¿°úÀûÀ¸·Î Æ÷À¯·ù¼¼Æ÷¿¡ µµÀÔÇÑ´Ù.
°íÈ¿À² transfection°ú ³·Àº ¼¼Æ÷µ¶¼º
È¿°úÀûÀÌ°í µ¶¼ºÀÌ ¾ø´Â DNA µµÀÔ ¹æ¹ýÀº ±âÃÊ ¿¬±¸ »Ó¸¸ ¾Æ´Ï¶ó À¯ÀüÀÚ µµÀÔ »ý¹° °³¹ßÀ̳ª À¯ÀüÀÚµµÀÔ Ä¡·á¿Í °°Àº ÀÀ¿ë ¿¬±¸¿¡¼µµ ²À ÇÊ¿äÇÑ ±â¼úÀÌ´Ù.
TransfectionÀÌ ¾î·Á¿î ¼¼Æ÷¿¡¼µµ ¶Ù¾î³ ¼º´É
ÀϹÝÀûÀ¸·Î »ç¿ëµÇ´Â ¿©·¯ Á¾·ùÀÇ cell lines¸¦ ÀÌ¿ëÇÏ¿© Xfect¿Í Ÿ»ç ½Ã¾àµéÀ» ºñ±³ÇÑ °á°ú, HeLa, HEK-293, CHO-K1, HT1080 ¼¼Æ÷¿¡¼ Xfect°¡ ¿ì¼öÇÑ transfection È¿À²À» ³ªÅ¸³Â´Ù (Figure 1). ¶ÇÇÑ, Xfect´Â ³·Àº ¼¼Æ÷ µ¶¼ºÀ¸·Î ³ôÀº ¼¼Æ÷ »ýÁ¸ ´É·Â (Table 1)À» ³ªÅ¸³Â´Ù. ´Ù¸¥ ºñ±³ ½ÇÇèÀ¸·Î´Â ÀϹÝÀûÀ¸·Î transfectionÀÌ ¾î·Æ´Ù°í ¾Ë·ÁÁø Jurkat ¼¼Æ÷¸¦ »ç¿ëÇÏ¿´´Ù. ±× °á°ú Xfect°¡ °æÀï»ç Á¦Ç° LXº¸´Ù 40 ¹è ÀÌ»óÀÇ ³ôÀº È¿À²À» ³ªÅ¸³Â´Ù.
Table I: Cells Transfected with Xfect have High Viability
Xfect (%)
Product L (%)
HeLa
79.4% ¡¾ 17.9
53.2 ¡¾ 26.7
HEK-293
63.5 ¡¾ 12.5
52.3 ¡¾ 12.4
CHO-K1
86 ¡¾ 0.8
90.9 ¡¾ 10.7
MCF7
51.4 ¡¾ 10.6
29.7 ¡¾ 21.3
°£ÆíÇÑ Á¶ÀÛ°úÁ¤
XfectÀº Ç÷û À¯¹«¿¡ »ó°ü¾øÀÌ transfectionÀ» ¼öÇà ÇÒ ¼ö ÀÖ´Ù. Transfection ½ÇÇè °úÁ¤Àº ÃÖ¼ÒÀÇ ´Ü°è·Î µÇ¾î ÀÖ´Ù.
Figure 1. Obtain high transfection efficiencies in many cell types with Xfect.
Xfect and Product L were used according to their respective protocols to transfect the indicated cell lines with an AcGFP1 expression vector. 48 hr posttransfection, the cultures were assessed for AcGFP1 expression using flow cytometry in order to determine transfection efficiency.
Components & Storage Conditions
°¢ Á¦Ç° ±¸¼º¹°°ú º¸°üÁ¶°ÇÀº Certificate of Analysis ¸¦ ÂüÁ¶ÇϽʽÿÀ.
Powered by Bioz
See more details on Bioz
Keyword :
´Ù¾çÇÑ ¼¼Æ÷/¹ü¿ëÀûÀÎ µµÀԽþà
¹ü¿ë¼º No.1, Plasmid DNA¿Í siRNA/miRNA ¸ðµÎ ..
Trans
IT-X2¢ç Transfection Reagent
Àúµ¶¼º No.1, µ¶¼º¿¡ ¹Î°¨ÇÑ ¼¼Æ÷µµ °íÈ¿À²·Î p..
Trans
IT¢ç-LT1 Transfection Reagent
Animal origin free·Î ¹ü¿ëÀûÀÎ »ç¿ë
Trans
IT¢ç-2020 Transfection Reagent
Mammalian cell ¿ë °íÈ¿À² ¡¤ Àúµ¶¼º Transfect..
Xfect¢â Transfection Reagent
CalPhos¢â Mammalian Transfection Kit