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Mammalian cell ¿ë °íÈ¿À² ¡¤ Àúµ¶¼º Transfection ½Ã¾à

Xfect¢â Transfection Reagent

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Clontech
631317
Xfect¢â Transfection Reagent
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50 ȸ¡¿2
357,000¿ø  Á¦Á¶»ç ÆäÀÌÁö·Î ¹Ù·Î°¡±â x32731, x94899, x32770, x32771, x33200
Clontech
631318
Xfect¢â Transfection Reagent
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100 ȸ¡¿3
868,000¿ø  Á¦Á¶»ç ÆäÀÌÁö·Î ¹Ù·Î°¡±â

  • ³ôÀº ¼öÁØÀÇ À¯ÀüÀÚ ¹ßÇö °¡´É
  • ³·Àº µ¶¼ºÀÇ »ýºÐÇؼº ³ª³ëÀÔÀÚ (biodegradable nonoparticles)
  • Ç÷û À¯¹«¿¡ »ó°ü¾øÀÌ »ç¿ë °¡´ÉÇÑ °£ÆíÇÑ Á¶ÀÛ °úÁ¤
Xfect´Â 2,300 Á¾·ù ÀÌ»óÀÇ polymer¸¦ ¼±º°ÇÏ¿© ¾ö¼±ÇÑ °íÈ¿À²ÀÇ transfection ½Ã¾àÀ̸ç, DNA°¡ °áÇÕÇÏ¿© »ýºÐÇؼº, ºñµ¶¼ºÀÇ ³ª³ëÀÔÀÚ¸¦ Çü¼ºÇÏ¿© DNA¸¦ È¿°úÀûÀ¸·Î Æ÷À¯·ù¼¼Æ÷¿¡ µµÀÔÇÑ´Ù.
°íÈ¿À² transfection°ú ³·Àº ¼¼Æ÷µ¶¼º
È¿°úÀûÀÌ°í µ¶¼ºÀÌ ¾ø´Â DNA µµÀÔ ¹æ¹ýÀº ±âÃÊ ¿¬±¸ »Ó¸¸ ¾Æ´Ï¶ó À¯ÀüÀÚ µµÀÔ »ý¹° °³¹ßÀ̳ª À¯ÀüÀÚµµÀÔ Ä¡·á¿Í °°Àº ÀÀ¿ë ¿¬±¸¿¡¼­µµ ²À ÇÊ¿äÇÑ ±â¼úÀÌ´Ù.
TransfectionÀÌ ¾î·Á¿î ¼¼Æ÷¿¡¼­µµ ¶Ù¾î³­ ¼º´É
ÀϹÝÀûÀ¸·Î »ç¿ëµÇ´Â ¿©·¯ Á¾·ùÀÇ cell lines¸¦ ÀÌ¿ëÇÏ¿© Xfect¿Í Ÿ»ç ½Ã¾àµéÀ» ºñ±³ÇÑ °á°ú, HeLa, HEK-293, CHO-K1, HT1080 ¼¼Æ÷¿¡¼­ Xfect°¡ ¿ì¼öÇÑ transfection È¿À²À» ³ªÅ¸³Â´Ù (Figure 1). ¶ÇÇÑ, Xfect´Â ³·Àº ¼¼Æ÷ µ¶¼ºÀ¸·Î ³ôÀº ¼¼Æ÷ »ýÁ¸ ´É·Â (Table 1)À» ³ªÅ¸³Â´Ù. ´Ù¸¥ ºñ±³ ½ÇÇèÀ¸·Î´Â ÀϹÝÀûÀ¸·Î transfectionÀÌ ¾î·Æ´Ù°í ¾Ë·ÁÁø Jurkat ¼¼Æ÷¸¦ »ç¿ëÇÏ¿´´Ù. ±× °á°ú Xfect°¡ °æÀï»ç Á¦Ç° LXº¸´Ù 40 ¹è ÀÌ»óÀÇ ³ôÀº È¿À²À» ³ªÅ¸³Â´Ù.
Table I: Cells Transfected with Xfect have High Viability
Xfect (%) Product L (%)
HeLa 79.4% ¡¾ 17.9 53.2 ¡¾ 26.7
HEK-293 63.5 ¡¾ 12.5 52.3 ¡¾ 12.4
CHO-K1 86 ¡¾ 0.8 90.9 ¡¾ 10.7
MCF7 51.4 ¡¾ 10.6 29.7 ¡¾ 21.3
°£ÆíÇÑ Á¶ÀÛ°úÁ¤
XfectÀº Ç÷û À¯¹«¿¡ »ó°ü¾øÀÌ transfectionÀ» ¼öÇà ÇÒ ¼ö ÀÖ´Ù. Transfection ½ÇÇè °úÁ¤Àº ÃÖ¼ÒÀÇ ´Ü°è·Î µÇ¾î ÀÖ´Ù.


Figure 1. Obtain high transfection efficiencies in many cell types with Xfect.
Xfect and Product L were used according to their respective protocols to transfect the indicated cell lines with an AcGFP1 expression vector. 48 hr posttransfection, the cultures were assessed for AcGFP1 expression using flow cytometry in order to determine transfection efficiency.
Components & Storage Conditions
°¢ Á¦Ç° ±¸¼º¹°°ú º¸°üÁ¶°ÇÀº Certificate of Analysis ¸¦ ÂüÁ¶ÇϽʽÿÀ.

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