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Home > ÀüÁ¦Ç°º¸±â > Transfection ½Ã¾à > small RNA Cloning > pBApo-CMV vector ½Ã¸®Áî
Æ÷À¯µ¿¹° ¼¼Æ÷ ¹ßÇö vector

pBApo-CMV vector ½Ã¸®Áî

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Á¦Á¶»ç Á¦Ç°ÄÚµå Á¦Ç°¸í ¿ë·® °¡°Ý
(ºÎ°¡¼¼º°µµ)
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Takara
3240
pBApo-CMV Neo DNA
°ü·ÃÇмú ±¸¸ÅÇϱâ
20 ¥ìg
338,400¿ø 
423,000¿ø
°¡°ÝÇÒÀÎ
09.02 ~ 10.31
3240_3242_e.v1612Da.pdf
Takara
3241
pBApo-CMV Pur DNA
°ü·ÃÇмú ±¸¸ÅÇϱâ
20 ¥ìg
338,400¿ø 
423,000¿ø
°¡°ÝÇÒÀÎ
09.02 ~ 10.31
3240_3242_e.v1612Da.pdf
Takara
3242
pBApo-CMV DNA
°ü·ÃÇмú ±¸¸ÅÇϱâ
20 ¥ìg
322,400¿ø 
403,000¿ø
°¡°ÝÇÒÀÎ
09.02 ~ 10.31
3240_3242_e.v1612Da.pdf

Á¦Ç°¼³¸í
pBApo-CMV´Â Æ÷À¯·ù ¼¼Æ÷¿ë À¯ÀüÀÚ expression vector·Î Cytomegalovirus À¯·¡ÀÇ promoter (CMV IE promoter) ¹× Herpes Simple Virus Thymidine KinaseÀÇ polyA signalÀ» žÀçÇÏ°í ÀÖ´Ù. Cloning site¿¡ ¸ñÀû À¯ÀüÀÚÀÇ ORF¸¦ »ðÀÔÇÏ¿© ¸ñÀû À¯ÀüÀÚÀÇ expression plasmid¸¦ ¾òÀ» ¼ö ÀÖÀ¸¸ç À¯ÀüÀÚ ¹ßÇö ÀÌ¿Ü¿¡ pri-microRNA µîÀÇ Àü»ç »ê¹° ¹ßÇö¿¡µµ ÀÌ¿ëÇÒ ¼ö ÀÖ´Ù.
¶ÇÇÑ ½±°Ô ¡¸promoter£«ORF£«polyA signal¡¹ cassette¸¦ adenovirus vector·Î ¿Å±æ ¼ö ÀÖÀ¸¸ç, À̶§ ÀçÁ¶ÇÕ adenovirus Á¦ÀÛ¿¡´Â Adenovirus Expression Vector Kit (Dual Version) Ver. 2 (Code 6170)¸¦ ÀÌ¿ëÇÒ ¼ö ÀÖ´Ù. Adenovirus´Â ³ôÀº °¨¿°È¿À²°ú ³ÐÀº °¨¿°´É·ÂÀ» °¡Áö°í ÀÖ¾î in vitro ¶Ç´Âin vivo À¯ÀüÀÚ µµÀÔ¿¡ ÀûÀýÇÏ´Ù.
pBApo-CMV ½Ã¸®Áî¿¡¼­´Â mammalian cellÀÇ selection marker·Î neomycin ³»¼º À¯ÀüÀÚ ¶Ç´Â puromycin ³»¼º À¯ÀüÀÚ¸¦ žÀçÇÑ vector°¡ ÀÖ¾î, ½ÇÇè ¸ñÀû¿¡ µû¶ó ÀûÀýÇÑ vector¸¦ ¼±ÅÃÇÒ ¼ö ÀÖ´Ù.
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pBApo-CMV Neo DNA (neomycin ³»¼ºÀ¯ÀüÀÚ Å¾Àç)
¶Ç´Â pBApo-CMV Pur DNA (puromycin ³»¼ºÀ¯ÀüÀÚ Å¾Àç) 20 ¥ìg
¶Ç´Â pBApo-CMV DNA (³»¼ºÀ¯ÀüÀÚ°¡ ¾ø´Â ±âº» vector)
º¸Á¸ -20¡É
°¢ DNAÀÇ ±¸Á¶


pBApo - CMV Neo , pBApo - CMV Pur ÀÇ Å¬·Î´× ±×¸²



pBApo - CMV ÀÇ Å¬·Î´× ±×¸²

Keyword :

small RNA Cloning
>
Æ÷À¯µ¿¹° ¼¼Æ÷ ¹ßÇö vector
pBApo-CMV vector ½Ã¸®Áî
>
Æ÷À¯µ¿¹° ¼¼Æ÷ ¹ßÇö vector
pBApo-EF1¥á Vector ½Ã¸®Áî
>
miRNA µî ÀúºÐÀÚ RNAÀÇ Cloning¿¡ ÃÖÀû
small RNA Cloning Kit
>
ÀúºÐÀÚ RNAÀÇ Cloning ¿ë ±âÁú Á¶Á¦¿¡ ÃÖÀû
small RNA Gel Extraction Kit