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Mouse ES ¼¼Æ÷¿¡ ÃÖÀûÈ­µÈ Transfection ½Ã¾à

Xfect mESC Transfection Reagent

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Á¦Á¶»ç Á¦Ç°ÄÚµå Á¦Ç°¸í ¿ë·® °¡°Ý
(ºÎ°¡¼¼º°µµ)
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Clontech
631320
Xfect mESC Transfection Reagent
°ü·ÃÇмú ±¸¸ÅÇϱ⠶óÀ̼±½º 
50 ȸ¡¿2
596,000¿ø  Á¦Á¶»ç ÆäÀÌÁö·Î ¹Ù·Î°¡±â x33194

  • Mouse embryonic stem cellÀü¿ë ÀÇ transfection ½Ã¾à
  • ÃÖ°íÀÇ È¿À²¼º - ³ôÀº ¼öÁØÀÇ À¯ÀüÀÚ ¹ßÇö
  • ¼¼Æ÷ µ¶¼ºÀÌ ¾ø´Â »ýºÐÇؼº ½Ã¾à - ¿øÇÏÁö ¾Ê´Â È¿°ú°¡ ¹èÁ¦µÈ »ý¹°ÇÐÀû °ü·Ã ÀÚ·á È®º¸ °¡´É
µ¶¼º ¾øÀÌ È¿°úÀûÀ¸·Î DNA¸¦ Àü´ÞÇÏ´Â °úÁ¤Àº À¯ÀüÀÚ Á¶Àý, ´Ü¹éÁú ¹ßÇö ¹× ±â´É ¿¬±¸, À¯ÀüÀÚº¯Çü »ý¹°Ã¼ÀÇ °³¹ß°ú Ä¡·á¸¦ ¸ñÀûÀ¸·Î ÇÏ´Â À¯ÀüÀÚ Àü´ÞÀ» Æ÷ÇÔÇÏ¿© ±âÃÊ ¿¬±¸¿Í ÀÀ¿ë ¿¬±¸¿¡ ÀÖ¾î ÇʼöÀûÀΠù ´Ü°èÀÌ´Ù. ÀϹÝÀûÀÎ transfection ½Ã¾àÀº ´Ù¾çÇÑ DNA¿Í ¸ñÀûÀ¸·Î ÇÏ´Â ¼¼Æ÷¿¡ ¹ü¿ëÀûÀ¸·Î »ç¿ëÇÒ ¼ö ÀÖÁö¸¸, ÃÖÀûÈ­µÈ ½Ã¾àÀº ƯÁ¤ ¼¼Æ÷¿¡ ÀÛ¿ëÇϵµ·Ï ¼³°èµÇ¾ú´Ù´Â Á¡ÀÌ À¯¸®ÇÏ°Ô ÀÛ¿ëÇÒ ¼ö ÀÖ´Ù.
Xfect StemÀº mouse embryonic stem cell (mES)ÀÇ transfection¿¡ ÃÖÀûÈ­µÇ¾î ÀÖ´Ù.
Çõ½ÅÀûÀÎ mES transfection ½Ã¾à
Xfect StemÀº ³ôÀº transfection È¿À²À» ³ªÅ¸³»´Â »õ·Î¿î »ýºÐÇؼº ³ª³ëÀÔÀÚ¸¦ ÀÌ¿ëÇÑ´Ù. 2,300 °³ ÀÌ»óÀÇ Èĺ¸ polymer¸¦ ¼±º°ÇÏ¿© ÃÖÀûÈ­µÈ º¹ÇÕü¸¦ °³¹ßÇÏ¿´´Ù.



Figure 1. Effective transfection of embryonic stem cells with Xfect mESC Transfection Reagent. ES-E14TG2a mES cells were transfected with 5 ¥ìg of DsRed-Express2 plasmid using 250 ¥ìg of Xfect mESC Transfection Reagent. 48 hr posttransfection, transfection efficiency was assessed via flow cytometry (left panel) as well as phase and fluorescent microscopy (middle and right panels respectively). As quantified by flow cytometric analysis, 93.9% of the cells transfected with Xfect mESC Transfection Reagent were positive for DsRed-Express2 expression (left panel), with a MFI of 716.9 (data not shown).
°íÈ¿À² & ³·Àº ¼¼Æ÷ µ¶¼º
Xfect StemÀº ¶Ù¾î³­ µµÀÔ È¿À² (Figure 1)°ú ³ôÀº »ýÁ¸´É·Â (viability)¸¦ Á¦°øÇÑ´Ù. °æÀï»çÀÇ ¼¼ °¡Áö transfection ½Ã¾à°ú ºñ±³ÇÏ¿´À» ¶§ Xfect StemÀº ES-E14TG2a (Figure 2)¿Í
ES-D3 µÎ °³ÀÇ mES ¼¼Æ÷¿¡¼­ ¸ðµÎ ÃÖ°íÀÇ transfection È¿À²À» ³ªÅ¸³»¾ú´Ù.



Figure 2. Xfect mESC Transfection Reagent provides superior transfection efficiency in mES cells. Xfect mESC Transfection Reagent and three competitor reagents were used according to their respective protocols to transfect mES cell lines using plasmid DNA encoding AcGFP1, in a 6-well format. 48 hr posttransfection, AcGFP1 expression was assessed by flow cytometry in order to determine transfection efficiency.

Figure 3. Xfect mESC Transfection Reagent exhibits far higher transfection efficiency than leading competitor reagents. ES-E14TG2a mES cells were transfected in a 6-well plate format with a plasmid expressing AcGFP1 using Xfect mESC Transfection Reagent (Panel A), Product L (Panel B), Product LX (Panel C), or Product F (Panel D) according to each manufacturer¡¯s recommended protocol. 48 hr posttransfection, the cells were imaged using white light (top) and by fluorescence microscopy using a Zeiss¢ç Axioskop™ microscope equipped with a GFP filter (bottom).
Components & Storage Conditions
°¢ Á¦Ç° ±¸¼º¹°°ú º¸°üÁ¶°ÇÀº Certificate of Analysis ¸¦ ÂüÁ¶ÇϽʽÿÀ.

Keyword : stem,Áٱ⼼Æ÷,Æ®·£½ºÆå¼Ç,transfection,mouse