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Transfection ½Ã¾à
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¼¼Æ÷¿¡ µû¸¥ È¿À²ÀûÀÎ À¯ÀüÀÚ µµÀÔ
> Xfect mESC Transfection Reagent
Mouse ES ¼¼Æ÷¿¡ ÃÖÀûÈµÈ Transfection ½Ã¾à
Xfect mESC Transfection Reagent
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Clontech
631320
Xfect mESC Transfection Reagent
50 ȸ¡¿2
596,000¿ø
Product index
Overview
[FAQ] Mirus transfecti..
Cell lineº° ¼±Åð¡À̵å
PACKAGE DELIVERY: The ..
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TransIT-X2¢ç Transfect..
TransIT¢ç-LT1 Transfec..
TransIT¢ç-2020 Transfe..
Xfect¢â Transfection R..
CalPhos¢â Mammalian Tr..
È¿À²Áõ´ë¸¦ À§ÇÑ Æ¯¼öµµÀÔ½Ã..
TransIT-VirusGEN¢ç Tra..
TransIT¢ç-Lenti Transf..
TransIT-PRO¢ç Transfec..
RevIT¢â AAV Enhancer
VirusGEN¢ç AAV Transfe..
VirusGEN¢ç LV Transfec..
¼¼Æ÷¿¡ µû¸¥ È¿À²ÀûÀÎ À¯Àü..
TransIT¢ç-293 Transfec..
TransIT¢ç-BrCa Transfe..
TransIT¢ç-CHO Transfec..
TransIT-HeLaMONSTER¢ç ..
TransIT¢ç-Jurkat Trans..
TransIT¢ç-Keratinocyte..
TransIT¢ç-Insect Trans..
Xfect mESC Transfectio..
Electroporation °ü·Ã
Ingenio¢ç solution & k..
Ingenio¢ç cuvette, ¼Ò..
µµÀÔ¹°Áú¿¡ µû¸¥ µµÀԽþà
TransIT¢ç-mRNA Transfe..
TransIT¢ç-Oligo Transf..
Xfect¢â RNA Transfecti..
Xfect¢â Protein Transf..
in-vivo delivery ½Ã½ºÅÛ
TransIT Hydrodynamic D..
µ¶¼º Á¦°Å - Transfection ..
MiraCLEAN¢ç Endotoxin ..
siRNA Transfection ½Ã¾à
Label IT Delivery Cont..
Label IT siRNA Tracker..
TransIT-QR Hydrodynami..
TransIT-siQUEST Transf..
TransIT-TKO Transfecti..
TransIT-X2 Dynamic Del..
siRNA ¹ßÇö ¡¤ ºÐ¼®
pSINsi vector ½Ã¸®Áî
Synthetic siRNA Quanti..
small RNA Cloning
pBApo-CMV vector ½Ã¸®..
pBApo-EF1¥á Vector ½Ã..
small RNA Cloning Kit
small RNA Gel Extracti..
RNAi °ü·Ã Kit
14-30 ssRNA Ladder Mar..
siRNA Ladder Marker
Mouse embryonic stem cellÀü¿ë ÀÇ transfection ½Ã¾à
ÃÖ°íÀÇ È¿À²¼º - ³ôÀº ¼öÁØÀÇ À¯ÀüÀÚ ¹ßÇö
¼¼Æ÷ µ¶¼ºÀÌ ¾ø´Â »ýºÐÇؼº ½Ã¾à - ¿øÇÏÁö ¾Ê´Â È¿°ú°¡ ¹èÁ¦µÈ »ý¹°ÇÐÀû °ü·Ã ÀÚ·á È®º¸ °¡´É
µ¶¼º ¾øÀÌ È¿°úÀûÀ¸·Î DNA¸¦ Àü´ÞÇÏ´Â °úÁ¤Àº À¯ÀüÀÚ Á¶Àý, ´Ü¹éÁú ¹ßÇö ¹× ±â´É ¿¬±¸, À¯ÀüÀÚº¯Çü »ý¹°Ã¼ÀÇ °³¹ß°ú Ä¡·á¸¦ ¸ñÀûÀ¸·Î ÇÏ´Â À¯ÀüÀÚ Àü´ÞÀ» Æ÷ÇÔÇÏ¿© ±âÃÊ ¿¬±¸¿Í ÀÀ¿ë ¿¬±¸¿¡ ÀÖ¾î ÇʼöÀûÀΠù ´Ü°èÀÌ´Ù. ÀϹÝÀûÀÎ transfection ½Ã¾àÀº ´Ù¾çÇÑ DNA¿Í ¸ñÀûÀ¸·Î ÇÏ´Â ¼¼Æ÷¿¡ ¹ü¿ëÀûÀ¸·Î »ç¿ëÇÒ ¼ö ÀÖÁö¸¸, ÃÖÀûÈµÈ ½Ã¾àÀº ƯÁ¤ ¼¼Æ÷¿¡ ÀÛ¿ëÇϵµ·Ï ¼³°èµÇ¾ú´Ù´Â Á¡ÀÌ À¯¸®ÇÏ°Ô ÀÛ¿ëÇÒ ¼ö ÀÖ´Ù.
Xfect StemÀº mouse embryonic stem cell (mES)ÀÇ transfection¿¡ ÃÖÀûȵǾî ÀÖ´Ù.
Çõ½ÅÀûÀÎ mES transfection ½Ã¾à
Xfect StemÀº ³ôÀº transfection È¿À²À» ³ªÅ¸³»´Â »õ·Î¿î »ýºÐÇؼº ³ª³ëÀÔÀÚ¸¦ ÀÌ¿ëÇÑ´Ù. 2,300 °³ ÀÌ»óÀÇ Èĺ¸ polymer¸¦ ¼±º°ÇÏ¿© ÃÖÀûÈµÈ º¹ÇÕü¸¦ °³¹ßÇÏ¿´´Ù.
Figure 1. Effective transfection of embryonic stem cells with Xfect mESC Transfection Reagent.
ES-E14TG2a mES cells were transfected with 5 ¥ìg of DsRed-Express2 plasmid using 250 ¥ìg of Xfect mESC Transfection Reagent. 48 hr posttransfection, transfection efficiency was assessed via flow cytometry (left panel) as well as phase and fluorescent microscopy (middle and right panels respectively). As quantified by flow cytometric analysis, 93.9% of the cells transfected with Xfect mESC Transfection Reagent were positive for DsRed-Express2 expression (left panel), with a MFI of 716.9 (data not shown).
°íÈ¿À² & ³·Àº ¼¼Æ÷ µ¶¼º
Xfect StemÀº ¶Ù¾î³ µµÀÔ È¿À² (Figure 1)°ú ³ôÀº »ýÁ¸´É·Â (viability)¸¦ Á¦°øÇÑ´Ù. °æÀï»çÀÇ ¼¼ °¡Áö transfection ½Ã¾à°ú ºñ±³ÇÏ¿´À» ¶§ Xfect StemÀº ES-E14TG2a (Figure 2)¿Í
ES-D3 µÎ °³ÀÇ mES ¼¼Æ÷¿¡¼ ¸ðµÎ ÃÖ°íÀÇ transfection È¿À²À» ³ªÅ¸³»¾ú´Ù.
Figure 2. Xfect mESC Transfection Reagent provides superior transfection efficiency in mES cells.
Xfect mESC Transfection Reagent and three competitor reagents were used according to their respective protocols to transfect mES cell lines using plasmid DNA encoding AcGFP1, in a 6-well format. 48 hr posttransfection, AcGFP1 expression was assessed by flow cytometry in order to determine transfection efficiency.
Figure 3. Xfect mESC Transfection Reagent exhibits far higher transfection efficiency than leading competitor reagents.
ES-E14TG2a mES cells were transfected in a 6-well plate format with a plasmid expressing AcGFP1 using Xfect mESC Transfection Reagent (Panel A), Product L (Panel B), Product LX (Panel C), or Product F (Panel D) according to each manufacturer¡¯s recommended protocol. 48 hr posttransfection, the cells were imaged using white light (top) and by fluorescence microscopy using a Zeiss¢ç Axioskop™ microscope equipped with a GFP filter (bottom).
Components & Storage Conditions
°¢ Á¦Ç° ±¸¼º¹°°ú º¸°üÁ¶°ÇÀº Certificate of Analysis ¸¦ ÂüÁ¶ÇϽʽÿÀ.
Keyword : stem,Áٱ⼼Æ÷,Æ®·£½ºÆå¼Ç,transfection,mouse
¼¼Æ÷¿¡ µû¸¥ È¿À²ÀûÀÎ À¯ÀüÀÚ µµÀÔ
Trans
IT¢ç-293 Transfection Reagent
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IT¢ç-BrCa Transfection Reagent
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IT¢ç-CHO Transfection Kit
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IT-HeLaMONSTER¢ç Transfection Kit
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IT¢ç-Jurkat Transfection Reagent
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IT¢ç-Keratinocyte Transfection Reagent
°í¿ª°¡ÀÇ Baculvoirus »ý»êÀ» À§ÇÑ Àü¿ë½Ã¾à
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IT¢ç-Insect Transfection Reagent
Mouse ES ¼¼Æ÷¿¡ ÃÖÀûÈµÈ Transfection ½Ã¾à
Xfect mESC Transfection Reagent