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ÀüÁ¦Ç°º¸±â
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Cloning °ü·Ã
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In-Fusion Cloning
> [NEW] In-Fusion¢ç Snap Assembly
ÃÖ°íÀÇ Colony Çü¼º´É°ú Á¤È®ÇÑ PCR cloning
[NEW] In-Fusion¢ç Snap Assembly
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Clontech
638947
In-Fusion¢ç Snap Assembly Master Mix
10 ȸ
210,750¿ø
281,000¿ø
05.02 ~ 06.30
Ưº°ÇÒÀÎ
,
Clontech
638948
In-Fusion¢ç Snap Assembly Master Mix
50 ȸ
897,000¿ø
1,196,000¿ø
05.02 ~ 06.30
Ưº°ÇÒÀÎ
Clontech
638949
In-Fusion¢ç Snap Assembly Master Mix
250 ȸ
2,916,750¿ø
3,889,000¿ø
05.02 ~ 06.30
Ưº°ÇÒÀÎ
Clontech
638951
In-Fusion¢ç Snap Assembly Master Mix with Competent Cells
10 ȸ
382,500¿ø
510,000¿ø
05.02 ~ 06.30
Ưº°ÇÒÀÎ
,
,
Clontech
638952
In-Fusion¢ç Snap Assembly Master Mix with Competent Cells
50 ȸ
1,762,500¿ø
2,350,000¿ø
05.02 ~ 06.30
Ưº°ÇÒÀÎ
Clontech
636763
Stellar¢â Competent Cells
10 ȸ
(10 x 100ul)
232,000¿ø
290,000¿ø
05.02 ~ 06.30
Clontech
636766
Stellar¢â Competent Cells
50 ȸ
(50 x 100ul)
986,400¿ø
1,233,000¿ø
05.02 ~ 06.30
Clontech
636767
Stellar¢â Competent Cells (96 well plate)
96 ȸ
(96 x 20ul)
632,000¿ø
790,000¿ø
05.02 ~ 06.30
Product index
½Ä¹°¿ë Vectors
Takara Plant ½ÇÇè °ü·Ã..
Agrobacterium tumefaci..
pRI 101 DNA ½Ã¸®Áî
pRI 201 DNA ½Ã¸®Áî
pRI 909/910 DNA
È¿¸ð ¡¤ °è»ó±Õ ¿ë Vectors
pAUR101 DNA
pAUR112 DNA
pAUR123 DNA
pAUR135 DNA
pAUR224 DNA
pAUR316 DNA
pPTR I DNA
pPTR II DNA
Genomic DNA
Genomic DNA
Thermus thermophilus H..
¥ëDNA
¥õX174 RF I DNA
DNA Ligation
Cloning ½ÇÇè ÀÔ¹®ÀÚ¸¦ ..
Takara Cloning FAQ
½ÇÇè ¸ñÀûº°·Î ÃßõÇÏ´Â..
DNA Blunting Kit
DNA Ligation Kit
DNA Ligation Kit £¼Mig..
TaKaRa DNA Ligation Ki..
TA-Cloning, ÆòȰ¸»´Ü Cloni..
½ÇÇè ¸ñÀûº°·Î ÃßõÇÏ´Â..
DNA Fragmentation Kit
Kilo-Sequence ¿ë Delet..
Mighty Cloning Reagent..
Mighty TA-cloning Kit
Mighty TA-cloning Reag..
TaKaRa LA PCR¢â in vit..
TaKaRa T-Vector pMD20
In-Fusion Cloning
[Overview] In-Fusion¢ç..
[°³¿ä] So Easy Product
Online Tools for In-Fu..
[Àû¿ë] In-Fusion¢ç Clo..
[FAQ] In-Fusion¢ç Clon..
[Tip] In-Fusion¢ç Clon..
½ÇÁ¦ °í°´ÀÇ »ç¿ëÈıâ
[NEW] In-Fusion¢ç Snap..
[NEW] µ¿°á°ÇÁ¶ In-Fusi..
[Kit] In-Fusion¢ç Clon..
[Plus] In-Fusion¢ç Clo..
In-Fusion¢ç Ready Vect..
Genomic DNA ÁõÆø¿ë Kit
GenomeWalkerKit
Vector (Cloning ¿ë)
M13 mp18 Single Strand..
pBR322 DNA
pKF18k-2/19k-3 DNA
pSTV28/29 DNA
pUC118/119 type vector
pUC18/19 type vector
TaKaRa T-Vector pMD20
Competent Cells & Electro-..
Takara Competent Cell ..
Agrobacterium tumefaci..
Chaperone Competent Ce..
E. coli Competent Cell..
E. coli Competent Cell..
E. coli Electro-Cells ..
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¼ö½ÄÈ¿¼Ò ¼øµµ À϶÷
¼ö½ÄÈ¿¼Òº° ¿ëµµ
¼ö½ÄÈ¿¼Ò£¯Ligase
E. coli DNA Ligase
T4 DNA Ligase
T4 RNA Ligase
¼ö½ÄÈ¿¼Ò£¯Alkaline Phospha..
Alkaline Phosphatase (..
Alkaline Phosphatase (..
Alkaline Phosphatase (..
T4 Polynucleotide Kina..
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DNA Polymerase I (E. ..
Klenow Fragment (Large..
mRNA Cap 2-O-Methyltra..
Poly(A) Polymerase
Pyrophosphatase (inorg..
SP6 RNA Polymerase
T4 DNA Polymerase
T7 RNA Polymerase
T7 RNA Polymerase ver...
Takara IVTpro¢â mRNA S..
Terminal Deoxynucleoti..
Vaccinia Capping Enzym..
¼ö½ÄÈ¿¼Ò£¯Nuclease¡¢Topois..
BAL 31 Nuclease
DNA Topoisomerase I
Exonuclease I
Exonuclease III
Micrococcal Nuclease
Mung Bean Nuclease
Recombinant DNase I (R..
Ribonuclease H (RNase ..
S1 Nuclease
Nucleotide
Deoxyribonucleoside 8..
dNTP Mixture
Ribonucleoside 5£§-Tri..
¹èÁö ¡¤ Buffer (ºÐ¸»)
AccuGENE 20¡¿SSPE Buff..
Bovine Serum Albumin S..
IPTG£¨dioxane free£©£¨..
X-Gal£¨5-Bromo-4-Chlor..
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Proteinase K
Westase (È¿¸ð ¼¼Æ÷º® ..
Yatalase
Yatalase -Plus-
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In-Fusion
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HD Cloning kit (Code 639648)
ÀÇ ¾÷±×·¹ÀÌµå ¹öÀü Á¦Ç°ÀÔ´Ï´Ù.
Subcloning ºÒÇÊ¿ä
- Cloning À§Ä¡, insert ¼¿¿¡ °ü°è¾øÀÌ, ¸ñÀû vector¿¡ ¹Ù·Î cloning
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Seamless construction
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Snap Assembly´Â ´Ü 15ºÐ ¹ÝÀÀÀ¸·Î ¼±ÇüÈ vector¿¡ ¾î¶² insert¶óµµ ºÒÇÊ¿äÇÑ ¿°±â Ãß°¡ ¾øÀÌ ¹æÇ⼺ ÀÖ´Â cloningÀÌ °¡´ÉÇÏ´Ù. Insert´Â Á¦ÇÑÈ¿¼Ò 󸮳ª phosphorylation, ȤÀº blunt-end ó¸® µî º°µµÀÇ ½ÇÇè °úÁ¤ÀÌ ÇÊ¿äÇÏÁö ¾Ê´Ù. In-Fusion
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Snap AssemblyÀÇ È¿À²Àº 95% ÀÌ»óÀ¸·Î, ÃæºÐÇÑ ¼öÀÇ colony¸¦ ¾òÀ» ¼ö ÀÖ¾î ´ë·® ºÐ¼®À» À§ÇÑ scale up¿¡µµ Àû¿ëÇÒ ¼ö ÀÖ´Ù.
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Snap Assembly Master Mix´Â ligase ¾øÀ̵µ, insert¿Í ¼±ÇüÈ vector ¾ç ¸»´Ü 15 bpÀÇ »óµ¿ ¿°±â¼¿À» ÀÌ¿ëÇÏ¿© È¿À²ÀûÀ¸·Î Á¤È®ÇÏ°Ô °áÇÕ½ÃŲ´Ù. ¿Â¶óÀο¡¼ Á¦°øÇÏ´Â
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primer design tool
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Master Mix´Â ¼±ÇüÈµÈ DNA °¡´ÚÀÇ 3’ ¸»´ÜÀ¸·ÎºÎÅÍ ¿°±â¼¿À» Á¦°ÅÇϰí, µÎ °³ÀÇ DNA ´ÜÆí¿¡¼ »óº¸¼¿ °£ÀÇ annealingÀ» ÅëÇØ DNA¸¦ °áÇÕ½ÃŲ´Ù. ÀÌ ¹æ¹ýÀº subcloningÀ̳ª º°µµÀÇ Á¶ÀÛ ¾øÀÌ, ¿©·¯ °³ÀÇ fragment¸¦ ÇϳªÀÇ vector¿¡ ¹Ù·Î Àû¿ëÇÒ ¼ö ÀÖ¾î multiple cloningÀÌ °¡´ÉÇÏ´Ù.
In-Fusion
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Snap Assembly Master Mix¿¡´Â master mix¿Í control vector, control insert°¡ Æ÷ÇԵǾî ÀÖ¾î cloning È¿À²À» °°ÀÌ È®ÀÎÇÒ ¼ö Àֱ⠶§¹®¿¡ ½ÇÇèÀÇ ½Å·Ú¼º ¶ÇÇÑ È®ÀÎÀÌ °¡´ÉÇÏ´Ù.
Stellar™ Competent Cells (Code 636763 ¿Ü)
˼ In-Fusion
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½Ã½ºÅÛ¿¡ ÃÖÀûȵǾî ÀÖÀ¸¸ç, In-Fusion
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Snap Assembly Master Mix with Competent Cells (Code 638951, 638952)¿¡´Â Stellar™ Competent CellsÀÌ Æ÷ÇԵǾî ÀÖ´Ù.
±×¸² 1. In-Fusion
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Workflow
±×¸² 2. In-Fusion
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Snap Assembly
¿Í In-Fusion HD (±âÁ¸ Á¦Ç°) °£ÀÇ È¿À² ºñ±³.
In-Fusion
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Snap Assembly´Â ±âÁ¸ Á¦Ç°¿¡ ºñÇØ Á¤È®µµ´Â À¯ÁöÇϸé¼, colony Çü¼º ´É·ÂÀÌ °³¼±µÇ¾ú´Ù.
Á¦Ç° ¼º´É ºñ±³¸¦ À§ÇØ In-Fusion
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HD (±âÁ¸ Á¦Ç°)°ú In-Fusion
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Snap AssemblyÀ» »ç¿ëÇÏ¿© ¼·Î ´Ù¸¥ Å©±âÀÇ inserts (405 bp-1,005 bp) 5°³¸¦ 2.7 kb vector¿¡ cloningÇÏ¿´´Ù. Cloning ¹ÝÀÀÀº °¢°¢ ¼¼ ¹ø ¹Ýº¹ ½ÇÇèÇÏ¿´°í, transformation ÈÄ °¢ ¹ÝÀÀÀ¸·ÎºÎÅÍ »ý¼ºµÈ colony¸¦ °¢ 20°³ ¼±ÅÃÇÏ¿© sanger sequencing ÇÑ °á°ú, In-Fusion
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Snap Assembly¿¡¼ ³ôÀº Á¤È®µµ¸¦ À¯ÁöÇÏ¸é¼ ±âÁ¸ Á¦Ç°º¸´Ù 4¹è ´õ ¸¹Àº colony¸¦ »ý¼ºÇÏ¿´´Ù. µÎ ½ÇÇè¿¡¼ In-Fusion
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master mix¸¦ Á¦¿ÜÇÑ primer¿Í ½Ã¾à µîÀº µ¿ÀÏÇÏ°Ô ÁøÇàÇÏ¿´´Ù.
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Snap Assembly
¿Í NEBuilder HiFi (1).
PCR·Î ¼±ÇüÈÇÑ vector¿¡ single insert¸¦ cloningÇÑ °á°ú, insert Å©±â¿¡ °ü°è¾øÀÌ NEBuilder HiFiº¸´Ù In-Fusion
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Snap Assembly¿¡¼ ÈξÀ ¸¹Àº colony ¼ö¸¦ º¸¿´°í, 95% ÀÌ»óÀ¸·Î ³ôÀº Á¤È®µµ¸¦ º¸¿´´Ù.
3.8 kb insert (A), 34.2 kb adenovirus insert (B)¸¦ 2.7 kbÀÇ ¼±ÇüÈ º¤ÅÍ¿¡ cloningÇÏ¿´´Ù. Cloning ¹ÝÀÀÀº °¢°¢ ¼¼ ¹ø ¹Ýº¹ ½ÇÇèÇÏ¿´°í, transformation ÈÄ °¢ ¹ÝÀÀÀ¸·ÎºÎÅÍ »ý¼ºµÈ colony¸¦ 20°³ ¼±ÅÃÇÏ¿© Sanger sequencing°ú colony PCR ¹æ¹ýÀ¸·Î cloning Á¤È®µµ¸¦ È®ÀÎÇÏ¿´´Ù. ±× °á°ú, In-Fusion
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Snap Assembly°¡ NEBuilder HiFi¿¡ ºñÇØ 2¹è ´õ ¸¹Àº colony¸¦ »ý¼ºÇÏ¿´´Ù.
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Snap Assembly¿Í NEBuilder HiFi (2).
Á¦ÇÑÈ¿¼Ò·Î ¼±ÇüÈÇÑ vector¿¡ single insert¸¦ cloningÇÑ °á°ú, ¸»´ÜÀÇ ÇüÅ (overhangs, blunt)¿¡ °ü°è¾øÀÌ NEBuilder HiFiº¸´Ù In-Fusion
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Snap Assembly¿¡¼ ÈξÀ ¸¹Àº colony ¼ö¸¦ º¸¿´´Ù.
In-Fusion
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Snap Assembly¿Í Ÿ»ç Á¦Ç° (NEBuilder HiFi)¸¦ ÀÌ¿ëÇØ Á¦ÇÑÈ¿¼Ò¸¦ ó¸®ÇÏ¿© Á¦ÀÛÇÑ 5’ overhang (A), blunt end (B), 3’ overhang (C)ÀÇ ´ÜÀÏ insert (3.8 kb)¸¦ ¼±ÇüÈÇÑ Ç¥ÁØ vector¿¡ cloningÇÏ¿´´Ù. Cloning ¹ÝÀÀÀº °¢°¢ ¼¼ ¹ø ¹Ýº¹ ½ÇÇèÇÏ¿´°í, °¢°¢ »ý¼ºµÈ colony¸¦ 20°³ ¼±ÅÃÇÏ¿© Sanger sequencingÀ¸·Î ¼¿ÀÌ Á¤È®ÇÏ°Ô »ðÀԵǾú´ÂÁö È®ÀÎÇÏ¿´´Ù.
Applications
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Snap Assembly Master MixÀÇ ´Ù¾çÇÑ applications ¿¹½Ã
ÀÚ¼¼ÇÑ ±¸¼ºÀº CoA¸¦ Âü°íÇϼ¼¿ä.
°ü·ÃÁ¦Ç°
- ÆíÀǼºÀÌ ÁÁÀº ½Ç¿Â º¸°ü master mix,
In-Fusion
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Snap Assembly EcoDry™ Master Mix (Code 638954)
- In-Fusion
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°ú ¦²á, ÃÖ°íÀÇ Á¤È®µµ¸¦ Áö´Ñ PCRÈ¿¼Ò,
PrimeSTAR
®
Max DNA Polymerase (Code R045A)
- In-Fusion
®
Cloning¿¡ ÃÖÀûÈµÈ °íÈ¿À² competent cells,
Stellar™ Competent Cells (Code 636763)
- Touch down PCR ¹× gradient PCR ¼³Á¤ÀÌ °¡´ÉÇÑ °£ÆíÇϰí ÄÄÆÑÆ®ÇÑ PCR ±â±â,
Clontech PCR Thermal Cycler GP (Code WN400)
- Plasmid DNA¸¦ ½Å¼ÓÇÏ°Ô Á¤Á¦ÇÒ ¼ö ÀÖ´Â,
TaKaRa MiniBEST Plasmid Purification Kit (Code 9760A ¿Ü)
Citations
In-Fusion
®
HD Cloning kit Á¦Ç°Àº In-Fusion
®
Snap Assembly Master Mix·Î ¾÷±×·¹ÀÌµå µÇ¾ú½À´Ï´Ù. In-Fusion
®
HD¿Í CitationÀÌ µ¿ÀÏÇÕ´Ï´Ù.
A highly photostable and bright green fluorescent protein
Nature Biotechnology | 2022 Apr 25 | PubMed ID: 35468954 |
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Distinct modes of mitochondrial metabolism uncouple T helper cell differentiation and function
Nature | 2019 Jul 05 | PubMed ID: 31217581 |
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Saturation Editing of Genomic Regions by Multiplex Homology-Directed Repair
Nature | 2014 Sep 04 | PubMed ID: 25141179 |
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Endocrine Deficiency As a Function of Radiation Dose to the Hypothalamus and Pituitary in Pediatric and Young Adult Patients With Brain Tumors
Journal of Clinical Oncology | 2018 Oct 01 | PubMed ID: 30118397 |
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SPARC enables genetic manipulation of precise proportions of cells.
Nature neuroscience | 2020 Jul 20 | PubMed ID: 32690967 |
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SARS-CoV-2 Infects the Brain Choroid Plexus and Disrupts the Blood-CSF Barrier in Human Brain Organoids
Cell Stem Cell | 2020 Oct 13 | PubMed ID: 33113348 |
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Mutations in SARS-CoV-2 variants of concern link to increased spike cleavage and virus transmission
Cell Host & Microbe | 2022 Jan 21 | PubMed ID: 35150638 |
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Chimeric CRISPR-CasX enzymes and guide RNAs for improved genome editing activity
Molecular cell | 2022 Jun 08 | PubMed ID: 35219382 |
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The Functional Proximal Proteome of Oncogenic Ras Includes mTORC2
Molecular cell | 2019 Jan 10 | PubMed ID: 30639242 |
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Identification of a novel deFADding activity in human, yeast and bacterial 5′ to 3′ exoribonucleases
Nucleic Acids Research | 2022 Aug 26 | PubMed ID: 35904778 |
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Experimental demonstration of operon formation catalyzed by insertion sequence
Nucleic Acids Research | 2022 Feb 22 | PubMed ID: 35066585 |
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Grand scale genome manipulation via chromosome swapping in Escherichia coli programmed by three one megabase chromosomes
Nucleic Acids Research | 2021 Sep 07 | PubMed ID: 33907814 |
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Drop-off-reinitiation triggered by EF-G-driven mistranslocation and its alleviation by EF-P
Nucleic Acids Research | 2022 Mar 21 | PubMed ID: 35188576 |
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Rational design of an XNA ligase through docking of unbound nucleic acids to toroidal proteins
Nucleic Acids Research | 2019 Jul 26 | PubMed ID: 31334814 |
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An siRNA-guided ARGONAUTE protein directs RNA polymerase V to initiate DNA methylation
Nature Plants | 2021 Nov 08 | PubMed ID: 34750500 |
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NIMA-related kinase 9 regulates the phosphorylation of the essential myosin light chain in the heart
Nature Communications | 2022 Oct 20 |
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Activation of stably silenced genes by recruitment of a synthetic de-methylating module
Nature Communications | 2022 Sep 23 | PubMed ID: 36151095 |
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Translational induction of ATF4 during integrated stress response requires noncanonical initiation factors eIF2D and DENR
Nature Communications | 2020 Sep 16 | PubMed ID: 32938929 |
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Age-dependent loss of adipose Rubicon promotes metabolic disorders via excess autophagy
Nature Communications | 2020 Aug 18 | PubMed ID: 32811819 |
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The surfaceome of multiple myeloma cells suggests potential immunotherapeutic strategies and protein markers of drug resistance
Nature Communications | 2022 Jul 15 | PubMed ID: 35840578 |
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Tracking cryptic SARS-CoV-2 lineages detected in NYC wastewater
Nature Communications | 2022 Feb 03 | PubMed ID: 35115523 |
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ICER is requisite for Th17 differentiation
Nature Communications | 2016 Sep 29 | PubMed ID: 27680869 |
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Therapeutic homology-independent targeted integration in retina and liver
Nature Communications | 2022 Apr 12 | PubMed ID: 35414130 |
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Robust and tunable signal processing in mammalian cells via engineered covalent modification cycles
Nature Communications | 2022 Mar 31 | PubMed ID: 35361767 |
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An endoribonuclease-based feedforward controller for decoupling resource-limited genetic modules in mammalian cells
Nature Communications | 2020 Nov 10 |
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Defunctionalizing intracellular organelles such as mitochondria and peroxisomes with engineered phospholipase A/acyltransferases
Nature Communications | 2022 Jul 29 | PubMed ID: 35906209 |
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A Single Immunization with Spike-Functionalized Ferritin Vaccines Elicits Neutralizing Antibody Responses against SARS-CoV-2 in Mice
ACS Central Science | 2021 Jan 05 |
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Regulatory encoding of quantitative variation in spatial activity of a Drosophila enhancer
Science Advances | 2020 Dec 02 | PubMed ID: 33268361 |
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Characterization of a vaccine-elicited human antibody with sequence homology to VRC01-class antibodies that binds the C1C2 gp120 domain
Science Advances | 2022 May 04 | PubMed ID: 35507661 |
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Exonuclease VII repairs quinolone-induced damage by resolving DNA gyrase cleavage complexes
Science Advances | 2021 Mar 03 | PubMed ID: 33658195 |
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A two-step mechanism governing PARP1-DNA retention by PARP inhibitors
Science Advances | 2022 Sep 01 | PubMed ID: 36070389 |
Read Article
>>
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Keyword : infusion,in-fusion,cloning,PCR cloning,cloning,Ŭ·Î´×,º¹¼ö´ÜÆí,ÀÎÇ»Àü,ÇÁ·Î¹Í½º,premix, mutagenesis,mutation,soeasy,so easy,639648,639649,639650,636763,638909,638910,638911
In-Fusion Cloning
[Overview] In-Fusion¢ç Cloning
[°³¿ä] So Easy Product
Online Tools for In-Fusion
[Àû¿ë] In-Fusion¢ç Cloning Àû¿ë»ç·Ê
[FAQ] In-Fusion¢ç Cloning
[Tip] In-Fusion¢ç Cloning
½ÇÁ¦ °í°´ÀÇ »ç¿ëÈıâ
ÃÖ°íÀÇ Colony Çü¼º´É°ú Á¤È®ÇÑ PCR cloning
[NEW] In-Fusion¢ç Snap Assembly
½Ç¿Â º¸°ü Master mix
[NEW] µ¿°á°ÇÁ¶ In-Fusion¢ç Snap Assembly
PremixÈ! ½Ã°£´ÜÃà! È¿À²¼º Çâ»ó!
[Kit] In-Fusion¢ç Cloning
[Plus] In-Fusion¢ç Cloning
In-Fusion¢ç cloningÀ» ¹Ù·Î ÁøÇàÇϱâ À§ÇÑ lin..
In-Fusion¢ç Ready Vectors