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dNTP Mixture

-

Á¦Á¶»ç Á¦Ç°ÄÚµå Á¦Ç°¸í ¿ë·® °¡°Ý
(ºÎ°¡¼¼º°µµ)
ºñ°í »ç¿ëÀڸŴº¾ó
Takara
4030
dNTP Mixture (ÊÀ2.5 mM)
°ü·ÃÇмú ±¸¸ÅÇϱâ
°¢ 3.2 ¥ìmol/
1.28 ml
69,600¿ø 
87,000¿ø
°¡°ÝÇÒÀÎ
09.02 ~ 10.31
4030_DS.v1812Da.pdf

º¸Á¸ -20¡É
- ¹Ýº¹ÀûÀÎ freeze-thaw¸¦ ÇÇÇÏ°í, ù¹ø° thawing ½Ã º°µµÀÇ tube¿¡ ºÐÁÖ ÈÄ -20¡É¿¡ º¸°ü ¹Ù¶ø´Ï´Ù.
³óµµ : °¢ 2.5 mM
Çü»ó : pH7~9ÀÇ ³ªÆ®·ý ¿° ¼ö¿ë¾×
¼øµµ
- 98% ÀÌ»ó
- PCR°ú RT-PCR ¹ÝÀÀ¿¡¼­ ¾çÈ£ÇÑ °á°ú°¡ ³ª¿À´Â °ÍÀ» È®ÀÎÇÏ¿´´Ù.
- RNase°¡ Æ÷ÇԵǾî ÀÖÁö ¾ÊÀ½À» È®ÀÎÇÏ¿´´Ù.
Ư¡
DNA polymeraseÀÇ ±âÁú·Î »ç¿ëÇÒ ¼ö ÀÖ´Ù.
  - ´ÜÇ°À¸·Î ÆǸÅÇÏ´Â dATP, dCTP, dGTP, dTTPÀÇ µ¿ÀÏÇÑ ¸ôºñÀÇ È¥ÇÕ¹°
  - Èñ¼®ÇÏÁö ¾Ê°í Á¦Ç° ±×´ë·Î PCR ¹ÝÀÀ¿¡ »ç¿ë °¡´É
  - PCR ¹ÝÀÀ¿¡ À־ Ç¥ÁØÀûÀÎ »ç¿ë·®Àº ¹ÝÀÀ¾×ÀÇ 100 §¡´ç 8 §¡ (ÃÖÁ¾³óµµ 200 μM)ÀÌ´Ù.
    (TaKaRa Taq™, TaKaRa Ex Taq®±âÁØ)
¿ëµµ
- DNA ÁõÆø (PCR)
- RT-PCR
- cDNA synthesis
- DNA cloning, sequencing ¹× labeling
°ü·ÃÁ¦Ç°
- °í³óµµ dNTP mixture Advantage® UltraPure PCR Deoxynucleotide Mix (10 mM each dNTP) (Code 639125)
- ºü¸¥ ¹ÝÀÀ¼Óµµ, ³ôÀº Á¤È®µµ, dye plus PCR È¿¼Ò premix PrimeSTAR® GXL Premix Fast, Dye plus (Code R052A)
- Gradient ±â´ÉÀ» žÀçÇÑ ÄÄÆÑÆ®ÇÑ PCR ±â±â TaKaRa PCR Thermal Cycler Dice® Touch (Code TP350)
- RoutineÇÏ°Ô »ç¿ë °¡´ÉÇÑ º£½ºÆ®¼¿·¯ agarose SeaKem® LE Agarose (Code 50004)
- Àú³óµµ »ùÇÿ¡ Àû¿ë °¡´ÉÇÑ 5x premix RTase, PrimeScript™ IV 1st strand cDNA Synthesis Mix (Code 6215A)

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