25 mM
Tris-HCl, pH7.6 (4¡É)
5 mM
MgCl2
50%
Glycerol
500 mM
Tris-HCl, pH9.0 (37¡É)
50 mM
1 M
Diethanolamine (pH9.8)
1 mM
p-nitrophenyl phosphate