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Cloning °ü·Ã
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È¿¸ð ¡¤ °è»ó±Õ ¿ë Vectors
> pAUR112 DNA
È¿¸ð ÀÚÀ²º¹Á¦Çü Suttle Vector
pAUR112 DNA
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Takara
3601
pAUR112 DNA
20 ¥ìg
469,000¿ø
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pUC118/119 type vector
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Competent Cells & Electro-..
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¼ö½ÄÈ¿¼Ò ¼øµµ À϶÷
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¼ö½ÄÈ¿¼Ò£¯Ligase
E. coli DNA Ligase
T4 DNA Ligase
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Alkaline Phosphatase (..
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Proteinase K
Westase (È¿¸ð ¼¼Æ÷º® ..
Yatalase
Yatalase -Plus-
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GenBank
Accession No. AB012283
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pAUR112 DNA´Â
Saccharomyces cerevisiae
¿¡¼ plasmid »óÅ·Π¾ÈÁ¤ÀûÀ¸·Î À¯ÁöµÇ´Â È¿¸ð shuttle vectorÀÌ´Ù. º» vector´Â ´ëÀå±Õ¿¡¼ÀÇ ¼±Åà ¸¶Ä¿·Î ampicillin ³»¼º À¯ÀüÀÚ Ampr ¸¦,
S. cerevisiae
¿¡¼ÀÇ ¼±Åà ¸¶Ä¿·Î AureobasidinA (AbA) ³»¼ºÀ¯ÀüÀÚ AUR1-C¿Í URA3À» °¡Áö¸ç ¾ÈÁ¤ÀûÀÎ ÀÚÀ²º¹Á¦¸¦ À§ÇØ CEN/ARS ¼¿·Î Æ÷ÇÔÇÏ°í ÀÖ´Ù. Ŭ·Î´× »çÀÌÆ®·Î
Xho
I,
Sal
I,
Xba
I,
Sma
I,
Sac
I,
Kpn
IÀÇ 6Á¾·ùÀÇ Á¦ÇÑÈ¿¼Ò Àý´ÜºÎÀ§¸¦ »ç¿ëÇÒ ¼ö ÀÖ´Ù.
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7,104 bp
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Ç×»ý¹°Áú Aureobasidin A¸¦ ÀÌ¿ëÇÑ È¿¸ðÀÇ ÇüÁúÀüȯÀ» À§ÇÑ vector. È¿¸ð ³»¿¡¼ plasmid »óÅ·Πº¸À¯ ¡¤ À¯ÁöµÈ´Ù.
License Notice : [M11]
pAUR112 DNAÀÇ ±¸Á¶
±×¸² pAUR112 DNA Á¦ÇÑÈ¿¼Ò ¸Ê ¹× cloning site
AUR1-C
S. cerevisiae ÀÇ AbA ³»¼º À¯ÀüÀÚ
URA3
S. cerevisiae ÀÇ Uracil ¼±Åà ¸¶Ä¿
ARS
S. cerevisiae º¹Á¦ ±âÁ¡
CEN
S. cerevisiae ÀÇ centromere
Amp
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E. coli ¿¡¼ÀÇ ¼±Åà ¸¶Ä¿
Ori
E. coli ¿¡¼ÀÇ º¹Á¦ ±âÁ¡
(pBR322 À¯·¡)
Keyword :
È¿¸ð ¡¤ °è»ó±Õ ¿ë Vectors
Chromosomal Integrating È¿¸ð Suttle Vector
pAUR101 DNA
È¿¸ð ÀÚÀ²º¹Á¦Çü Suttle Vector
pAUR112 DNA
È¿¸ð·Î ´Ü¹éÁú ¹ßÇöÇü Shuttle Vector
pAUR123 DNA
Ãâ¾Æ È¿¸ð ¿ë Marker Á¦°ÅÇü Vector
pAUR135 DNA
Schizo. pombe ¿ë ´Ü¹éÁú ¹ßÇöÇü Shuttle Vecto..
pAUR224 DNA
Aspergillus ¿ë Shuttle Vector
pAUR316 DNA
Chromosomal Integrating Çü Shuttle Vector
pPTR I DNA
ÀÚÀ² º¹Á¦Çü Shuttle Vector
pPTR II DNA