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Cloning °ü·Ã
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½Ä¹°¿ë Vectors
> pRI 101 DNA ½Ã¸®Áî
½Ä¹° ÇüÁúÀüȯ¿ë °í¹ßÇö Vector
pRI 101 DNA ½Ã¸®Áî
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Takara
3262
pRI 101-AN DNA
10 ¥ìg
662,000¿ø
Takara
3263
pRI 101-ON DNA
10 ¥ìg
662,000¿ø
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* Takara Plant ½ÇÇè °ü·ÃÁ¦Ç° overview (Ŭ¸¯)
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pRI 101-AN DNA 10 §¶
pRI 101-ON DNA 10 §¶
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pRI101 DNA ½Ã¸®Áî´Â ÇüÁúÀüȯ ½Ä¹°¿¡¼ ¿Ü·¡À¯ÀüÀÚ¸¦ °íÈ¿À²·Î ¹ßÇö½ÃÅ°±â À§ÇÑ vectorÀÌ´Ù. Cauliflower mosaic virus (CaMV)ÀÇ 35S promoter ¾Æ·¡ÂÊ¿¡ ADH (Alcohol Dehydrogenase) À¯ÀüÀÚ À¯·¡ÀÇ 5¡¯ untranslated region (5¡¯-UTR)À» Æ÷ÇÔÇÏ°í ÀÖÀ¸¸ç, ÇØ´ç ¿µ¿ªÀÌ °¡Áö´Â translation enhancer ±â´É¿¡ ÀÇÇØ ½Ä¹°Ã¼¿¡¼ ¸ñÀûÀ¯ÀüÀڻ깰À» °íÈ¿À²·Î ¹ßÇö½Ãų ¼ö ÀÖ´Ù. ¾Ö±âÀå´ë ADH À¯·¡ 5¡¯-UTR (AtADH 5¡¯-UTR)À» žÀçÇÑ pRI101-AN DNA¿Í º ADH À¯·¡ 5¡¯-UTR (OsADH 5¡¯-UTR)À» žÀçÇÑ pRI101-ON DNAÀÇ 2Á¾·ù°¡ ÀÖ¾î¼ ½Ö¶±ÀÙ ½Ä¹°¿¡´Â pRI101-AN DNA¸¦, ¿Ü¶±ÀÙ ½Ä¹°¿¡´Â pRI101-ON DNA¸¦ ÀÌ¿ëÇÒ ¼ö ÀÖ´Ù.
¶ÇÇÑ º» Á¦Ç°Àº ½Ä¹°ÇüÁúÀüȯ¿ë binary vectorÀÎ pRI910 DNA (Code 3261)À» ±âº»À¸·Î ÇÏ´Â binary vectorÀ̸ç,
Rhizobium rhizogenes
Ri plasmid À¯·¡ÀÇ º¯ÀÌÇü º¹Á¦ ±âÁ¡(Ri ori)À» °¡Áö°í ÀÖ´Ù. pUC °èÀÇ plasmid¿Í °°Àº º¹Á¦ ±âÁ¡ (ColE1 ori)µµ °¡Áö°í ÀÖ¾î ´ëÀå±Õ¿¡¼ ³ôÀº Ä«ÇǼöÀÇ plasmid·Î À¯ÁöµÇ¸ç, cloning site°¡ T-DNA³» ½Ä¹°¼±Åø¶Ä¿ÀÇ right border (RB)Ãø¿¡ ¹èÄ¡µÇ¾î Àֱ⠶§¹®¿¡ ¸ñÀû À¯ÀüÀÚ°¡ °á½ÇµÇÁö ¾Ê°í ¾ÈÁ¤ÀûÀ¸·Î ½Ä¹° ¿°»öü¿¡ »ðÀÔÀÌ °¡´ÉÇÏ´Ù.
º» º¤ÅÍ´Â ±¹¸³ ´ëÇÐ ¹ýÀÎ ³ª¶ó (ÑÕÕÞ) ÷´Ü°úÇбâ¼ú´ëÇпø´ëÇп¡¼ ±â¼ú ¹× ½ÇÇèÀÚ·áÀÇ Á¦°øÀ» ¹Þ°í, ´ÙÄ«¶ó¹ÙÀÌ¿À°¡ Á¦Ç°ÈÇÏ¿´½À´Ï´Ù.
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Double strand covalently closed circular form (RF I)À» 70%ÀÌ»ó Æ÷ÇÔÇÏ°í ÀÖ´Ù.
Dideoxy¹ý¿¡ ÀÇÇÑ ½ÃÄö½Ì °á°ú Ŭ·Î´× »çÀÌÆ®¸¦ À¯ÁöÇÏ°í ÀÖ´Ù´Â °ÍÀ» È®ÀÎÇÏ°í ÀÖ´Ù.
Cloning site¿¡¼¸¸ 1°÷À» Àý´ÜÇÏ´Â Á¦ÇÑ È¿¼Ò¿¡ ÀÇÇØ ÇØ´ç Àý´ÜÀ» È®ÀÎÇÏ°í ÀÖ´Ù.
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Binary vector¹ý¿¡ ÀÇÇØ Agrobacterium À» ¸Å°³·Î ½Ä¹°À» ÇüÁúÀüȯÇϱâ À§ÇÑ vector.
¾Ö±âÀå´ë, Å丶Åä, ´ã¹è µîÀÇ ½Ö¶±ÀÙ ½Ä¹° (pRI101-AN DNA¸¦ »ç¿ë) ¶Ç´Â º µîÀÇ ¿Ü¶±ÀÙ ½Ä¹° (pRI101-ON DNA¸¦ »ç¿ë)ÀÇ ÇüÁúÀüȯ ¹× ¿Ü·¡ À¯ÀüÀÚÀÇ ³ôÀº ¹ßÇöÀ» ½ÇÇö.
pRI 101-AN DNA¿Í pRI 101-ON DNA
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Takara Plant ½ÇÇè °ü·ÃÁ¦Ç°
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Agrobacterium tumefaciens LBA4404 Electro-Cells
½Ä¹° ÇüÁúÀüȯ¿ë °í¹ßÇö Vector
pRI 101 DNA ½Ã¸®Áî
½Ä¹° ÇüÁúÀüȯ¿ë °í¹ßÇö Vecto£¨New version£©
pRI 201 DNA ½Ã¸®Áî
½Ä¹° ÇüÁúÀüȯ¿ë Binary Vector
pRI 909/910 DNA