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TaKaRa
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Cloned

T4 Polynucleotide Kinase

-

Á¦Á¶»ç Á¦Ç°ÄÚµå Á¦Ç°¸í ¿ë·® °¡°Ý
(ºÎ°¡¼¼º°µµ)
ºñ°í »ç¿ëÀڸŴº¾ó
Takara
2021A
T4 Polynucleotide Kinase
°ü·ÃÇмú ±¸¸ÅÇϱâ
1,000 U
75,200¿ø 
94,000¿ø
°¡°ÝÇÒÀÎ
09.02 ~ 10.31
2021A_DS.v2106Da.pdf
Takara
2021B
T4 Polynucleotide Kinase
°ü·ÃÇмú ±¸¸ÅÇϱâ
5,000 U (2021A x 5)
425,000¿ø 

Á¦Ç°¼³¸í
º» È¿¼Ò´Â DNA ȤÀº RNA 5¡¯-¸»´ÜÀÇ ATP ¥ãÀ§Ä¡¿¡ Àλê±â¸¦ ÷°¡ÇÏ´Â È¿¼ÒÀÌ´Ù. ATP°¡ Á¸ÀçÇÒ ¶§ 5¡¯-OH ¸»´ÜÀÇ ÀλêÈ­ ¹ÝÀÀÀÌ ÀϾ´Ù. À̶§ Ç¥½Ä µÈ ATP¸¦ ÷°¡Çϸé ÀλêÈ­ ÀüÀ̹ÝÀÀÀÌ µ¿½Ã¿¡ ÀϾ 5¡¯-¸»´ÜÀÌ Ç¥½Ä µÈ Àλê±â·Î ±³È¯µÈ´Ù (±³È¯¹ÝÀÀ).
º¸Á¸ -20¡É
³óµµ 10 U/§¡
Çü»ó

50 mM

 

Tris-HCl (pH7.5)

50 mM

 

KCl

1 mM

 

DTT

0.1 ¥ìM

 

ATP

50%

 

Glycerol

÷ºÎ buffer ±¸¼º (10x)

500 mM

 

Tris-HCl (pH8.0)

100 mM

 

MgCl 2

50 mM

 

DTT

Ç¥½ÄÇÏÁö ¾Ê°í ÀλêÈ­ ¹ÝÀÀÀ» ½Ç½ÃÇÒ ¶§´Â ¹ÝÀÀ¾×¿¡ ÃÖÁ¾ ³óµµ 1 mM·Î ATP¸¦ ÷°¡ÇÏ¿© ÁֽʽÿÀ.
º» È¿¼Ò¿¡´Â ATP°¡ ÷ºÎµÇ¾î ÀÖÁö ¾Ê½À´Ï´Ù (ATP (Code 4041) ÀÌ¿ë °¡´É).
È°¼ºÀÇ Á¤ÀÇ
Micrococcal nuclease¿¡¼­ È°¼ºÈ­ÇÑ ¼Û¾ÆÁö Èä¼± DNAÀ» ±âÁú·Î ÇÏ¿©, 37¡É, pH7.6¿¡¼­ 30 ºÐ°£ 1 nmolÀÇ [¥ã- 32P]ATP¸¦ »êºÒ¿ë¼º ħÀü¹°·Î ¹Ù²Ù´Â È¿¼ÒÈ°¼ºÀ» 1UÀ¸·Î ÇÑ´Ù.
È°¼º ÃøÁ¤¿ë ¹ÝÀÀ¾× Á¶¼º

50 mM

 

Tris-HCl (pH7.6)

10 mM

 

MgCl2

1 mM

 

DTT

100 ¥ìM

 

[¥ã- 32P]ATP

0.2 §·/§¢

 

5¡¯-OH DNA

¿ëµµ
- DNA ¶Ç´Â RNAÀÇ 5¡¯-¸»´Ü Ç¥½Ä
- ÇÕ¼º DNA linkerÀÇ 5¡¯-¸»´Ü ÀλêÈ­
±â¿ø
Escherichia coli carrying the plasmid encoding T4 pse T gene
ÀϹÝÀû ¼ºÁú
- ºÐÀÚ·®: ¾à 140,000
- Subunit
   ºÐÀÚ·® ¾à 33,000ÀÇ subunit homo oligomer
   È°¼º ¹ßÇö ½Ã¿¡´Â tetramer·Î ÃßÁ¤
- ÃÖÀû pH
   ÀλêÈ­ ¹ÝÀÀ: pH6.5 ~ pH8.5
   ±³È¯ ¹ÝÀÀ: pH6.2 ~ pH6.6
   Å» ÀλêÈ­ ¹ÝÀÀ: pH6.0
- º¸Á¶ÀÎÀÚ
   Mg2+(10 mM, pH7.6)¸¦ ÇÊ¿ä·Î ÇÔ, ±× ¿Ü¿¡ Mn2+, Zn2+ µµ »ç¿ë °¡´É
   È¯¿øÁ¦ Çʿ伺
  • 5 mM DTT¿¡¼­ ÃÖ´ë È°¼ºÀ» ³ªÅ¸³½´Ù.
  • 10 mM 2-mercaptoethanol ¶Ç´Â 10 mM glutathioneÀ¸·Î ´ëüÇÒ ¼ö ÀÖÁö¸¸
    °¢°¢ 5 mM DTT ´ëºñ È°¼ºÀÌ 80%, 70% ÀúÇϵȴÙ.
- ÀúÇØÁ¦
   Àλê, pyrophosphoric acid, trisodium phosphate: 7 mM, 50% ¾ïÁ¦
   È²»ê¾Ï¸ð´½: 7 mM 75%¾ïÁ¦

Keyword :

¼ö½ÄÈ¿¼Ò£¯Alkaline Phosphatase¡¢Polynucleotide Kinase