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Home > ÀüÁ¦Ç°º¸±â > Cloning °ü·Ã > DNA Ligation > ½ÇÇè ¸ñÀûº°·Î ÃßõÇÏ´Â Cloning Kit

½ÇÇè ¸ñÀûº°·Î ÃßõÇÏ´Â Cloning Kit

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Cloning Kit ¼±Åð¡À̵å



[½ÇÇè ¸ñÀûº° Cloning Kit ¼±ÅÃÇϱâ]


½Å¼Ó, °£Æí, °íÈ¿À² ligation
Blunt end ligation
Long insert (>10 kb ligation)


3¡¯ ¸»´Ü¿¡ dA°¡ ºÎ°¡µÈ
insert ligation
3¡¯ ¸»´Ü¿¡ dA°¡ ¾ø´Â
insert ligation
; PrimeSTAR¢ç µî high fidelity È¿¼Ò¸¦
ÀÌ¿ëÇÑ °æ¿ì


½Å¼Ó, °íÈ¿À² cloning
In-Fusion¢ç Snap Assembly Master Mix
- »óµ¿¼­¿­À» ÀçÁ¶ÇÕÇÏ¿© ´Ü 15ºÐ¸¸¿¡ cloning ¿Ï·á
- »ç¿ëÇÒ vectorÀÇ ¾ç ¸»´Ü ¼­¿­À» ÀÌ¿ëÇϹǷΠ¿°±âÃß°¡ ºÒÇÊ¿ä
- Multiple fragment, large insert cloning¿¡µµ ÃÖÀû
Multiple fragment cloning
Mutagenesis


[Ligation, dephosphorylation ¿¡ »ç¿ëÇÏ´Â È¿¼Ò]




[In-Fusion CloningÀ» À§ÇÑ ¿Â¶óÀÎ Åø]