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È¿¸ð·Î ´Ü¹éÁú ¹ßÇöÇü Shuttle Vector

pAUR123 DNA

-

Á¦Á¶»ç Á¦Ç°ÄÚµå Á¦Ç°¸í ¿ë·® °¡°Ý
(ºÎ°¡¼¼º°µµ)
ºñ°í »ç¿ëÀڸŴº¾ó
Takara
3602
pAUR123 DNA (È¿¸ð ¹ßÇöÇü Vector)
°ü·ÃÇмú ±¸¸ÅÇϱ⠶óÀ̼±½º 
20 ¥ìg
375,200¿ø 
469,000¿ø
°¡°ÝÇÒÀÎ
09.02 ~ 10.31
3602_DS.v2109Da.pdf

³óµµ : 1 mg/ §¢
º¸Á¸ : -20¡É
GenBank
Accession No. AB012284
Á¦Ç°¼³¸í
º» Á¦Ç°Àº ÀÚÀ² º¹Á¦Çü º¤ÅÍ pAUR112 À¯·¡ÀÇ ´Ü¹éÁú ¹ßÇö¿ë plasmid vector·Î¼­ Saccharomyces cerevisiae ³»¿¡¼­ plasmid »óÅ·Π¾ÈÁ¤ÇÏ°Ô À¯ÁöÇÒ ¼ö ÀÖ´Â È¿¸ð shuttle vectorÀÌ´Ù. ¹ßÇö¿ë promoter·Î¼­ ±¸¼ºÀûÀ¸·Î ¹ßÇöÇÏ´Â alcohol dehydrogenase I À¯ÀüÀÚ (ADH1)ÀÇ promoter¸¦ ÇÔÀ¯ÇÏ°í ÀÖ´Ù. º» vector´Â ´ëÀå±ÕÀÇ ¼±Åà ¸¶Ä¿·Î ampicillin ³»¼ºÀ¯ÀüÀÚ¸¦, ¶Ç S. cerevisiaeÀÇ ¼±Åà ¸¶Ä¿·Î Aurobasidin A (AbA) ³»¼º À¯ÀüÀÚ AUR1-C¸¦ °®°í ÀÖÀ¸¸ç ¾ÈÁ¤ÀûÀÎ ÀÚÀ²º¹Á¦¿¡ ÇÊ¿äÇÑ CEN/ARS ¼­¿­À» °¡Áö°í ÀÖ´Ù. ¹ßÇöÇÏ°íÀÚ ÇÏ´Â À¯ÀüÀÚ¸¦ °³½Ã codon ATG¸¦ ÇÔÀ¯ÇÑ ÇüÅ·Πcloning site¿¡ »ðÀÔÇÔÀ¸·Î½á È¿¸ð ³»¿¡¼­ ±¸¼ºÀûÀ¸·Î ¹ßÇöÇÒ ¼ö ÀÖ´Ù. ¶Ç cloning site¿¡´Â reading frameÀ» shift½ÃŲ 3°³ÀÇ stop codonÀÌ µé¾î ÀÖ´Ù.
±æÀÌ
6,982 bp
¿ëµµ
Ç×»ý¹°Áú Aureobasidin A¸¦ ÀÌ¿ëÇÑ È¿¸ðÀÇ ÇüÁúÀüȯ¿ë º¤ÅÍ(´Ü¹éÁú ¹ßÇöÇü shuttle vector). È¿¸ð ³»¿¡¼­ plasmid »óÅ·Πº¸À¯¡¤ À¯ÁöµÈ´Ù.
»ç¿ë»óÀÇ ÁÖÀÇ
º» vector¿¡ »ç¿ëÇÏ´Â Aph1 promoter´Â °í¹ßÇö ÇüÅÂÀÇ promoter°¡ ¾Æ´Ï¹Ç·Î ¸ñÀû ´Ü¹éÁúÀÇ °í¹ßÇöÀº ±â´ëÇÒ ¼ö ¾ø´Ù.
License Notice : [M11]
pAUR123 DNAÀÇ ±¸Á¶

±×¸² pAUR123 DNA Á¦ÇÑÈ¿¼Ò ¸Ê ¹× cloning site

AUR1-C S. cerevisiaeÀÇ AbA ³»¼º À¯ÀüÀÚ
PADH1 S. cerevisiaeÀÇ ADH1 À¯ÀüÀÚÀÇ promoter
TADH1 S. cerevisiaeÀÇ ADH1 À¯ÀüÀÚÀÇ terminator
ARS S. cerevisiaeÀÇ º¹Á¦ ±âÁ¡
CEN S. cerevisiaeÀÇ centromere
Ampr E. coli¿¡¼­ÀÇ ¼±Åà ¸¶Ä¿
Ori E. coli¿¡¼­ÀÇ º¹Á¦ ±âÁ¡(pBR322 À¯·¡)

Keyword :

È¿¸ð ¡¤ °è»ó±Õ ¿ë Vectors
>
Chromosomal Integrating È¿¸ð Suttle Vector
pAUR101 DNA
>
È¿¸ð ÀÚÀ²º¹Á¦Çü Suttle Vector
pAUR112 DNA
>
È¿¸ð·Î ´Ü¹éÁú ¹ßÇöÇü Shuttle Vector
pAUR123 DNA
>
Ãâ¾Æ È¿¸ð ¿ë Marker Á¦°ÅÇü Vector
pAUR135 DNA
>
Schizo. pombe ¿ë ´Ü¹éÁú ¹ßÇöÇü Shuttle Vecto..
pAUR224 DNA
>
Aspergillus ¿ë Shuttle Vector
pAUR316 DNA
>
Chromosomal Integrating Çü Shuttle Vector
pPTR I DNA
>
ÀÚÀ² º¹Á¦Çü Shuttle Vector
pPTR II DNA