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DNA ¸»´Ü ÆòÈ°È­¿¡

DNA Blunting Kit

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Takara
6025
DNA Blunting Kit
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20 ȸ
212,000¿ø 
265,000¿ø
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09.02 ~ 10.31
6025_e.v2001Da.pdf

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T4 DNA Polymerase 20 §¡
10¡¿ Buffer (dNTP Æ÷ÇÔ) 30 §¡
DNA Dilution Buffer 500 §¡
Ligation Solution A* 600 §¡¡¿2
Ligation Solution B* 150 §¡
*Ligation Solution A, B´Â DNA Ligation Kit Ver.1 (TaKaRa Code 6021)ÀÇ ½Ã¾à°ú °°´Ù.
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DNA Blunting Kit´Â T4 DNA polymeraseÀÇ 5¡¯¡æ 3¡¯polymerase È°¼º°ú 3¡¯¡æ 5¡¯ exonuclease È°¼ºÀ» ÀÌ¿ëÇÏ¿© DNA ¸»´ÜÀ» ÆòÈ°È­ÇÔÀ¸·Î½á µ¹Ã⸻´Ü DNA¸¦ °£´ÜÈ÷ ÆòÈ°¸»´ÜÀÇ vector¿¡ ¿¬°áÇÏ´Â ½Ã½ºÅÛÀÌ´Ù.
DNA°¡ 5¡¯µ¹Ã⸻´ÜÀÇ °æ¿ì´Â 5¡¯¡æ 3¡¯polymerase È°¼º¿¡ ÀÇÇØ 3¡¯ÇÔ¸ôºÎºÐ¿¡ ¿°±â°¡ ºÎ°¡µÇ°í, 5¡¯ÇÔ¸ô¸»´ÜÀÇ °æ¿ì´Â 3¡¯¡æ 5¡¯ exonuclease È°¼º¿¡ ÀÇÇØ 3¡¯µ¹ÃâºÎºÐÀ» ºÐÇØÇÏ¿© ÆòÈ°¸»´ÜÈ­ÇÑ´Ù. ÀÌ ¸»´Ü ÆòÈ°È­ ¹ÝÀÀÀº µ¿½Ã¿¡ ÀϾ¹Ç·Î ¸»´ÜÇü»óÀÌ ºÒºÐ¸íÇÑ DNA¶óµµ °£´ÜÈ÷ ÆòÈ°È­ÇÒ ¼ö ÀÖ´Ù. ÆòÈ°¸»´ÜÈ­ÇÑ DNA´Â TaKaRa µ¶ÀÚÀÇ ligation ½Ã½ºÅÛÀ» ÅëÇÏ¿© °íÈ¿À²·Î ÆòÈ°¸»´ÜÀÇ vector µî¿¡ ¿¬°áÇÒ ¼ö ÀÖ¾î PCR ÁõÆø ÈÄÀÇ DNA³ª Ŭ·Î´×¿¡ ºÎÀû´çÇÑ ¸»´ÜÀ» °®´Â »ðÀÔ DNA¸¦ ÆòÈ°¸»´Ü vector¿¡ ligationÇÒ ¶§¿¡ ƯÈ÷ À¯¿ëÇÏ´Ù.
º» Á¦Ç°Àº µ¹Ã⸻´Ü vector¸¦ ÆòÈ°¸»´Ü vector·Î º¯°æÇÒ °æ¿ì¿¡µµ È¿°úÀûÀÌ´Ù. ÆòÈ°È­½ÃŲ vector ´Â Alkaline Phosphatase (Code 2120A/B, 2250A/B)·Î Å»ÀλêÈ­ÇÏ¿© »ç¿ëÇÑ´Ù.

Keyword :

DNA Ligation
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DNA ¸»´Ü ÆòÈ°È­¿¡
DNA Blunting Kit
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°íÈ¿À²ÀÇ Ligation premix
DNA Ligation Kit £¼Mighty Mix£¾
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10 kb ÀÌ»óÀÇ insert cloning¿¡ ÃÖÀû
TaKaRa DNA Ligation Kit LONG