´Ý±â
TaKaRa
Á¦Ç°¸í/Á¦Ç°ÄÚµå
Å°¿öµå°Ë»ö
°Ë»ö
´Ý±â
  • °í°´Áö¿ø 
  • ¾÷¹«¾È³»¦¢Á¦Ç°¹®ÀÇ
  • ÀüÈ­¹øÈ£¦¢02-2081-2510
  • Email     ¦¢support@takara.co.kr
  • ´ëÀüÁö»ç 
  • ¾÷¹«¾È³»¦¢´ëÀü/ÃæûÁö¿ª ÁÖ¹®, Á¦Ç°¹®ÀÇ
  • ÀüÈ­¹øÈ£¦¢042-828-6525
  • Email     ¦¢tkbd@takara.co.kr
  • ¾÷¹«½Ã°£¾È³»
  • [ Æò¡¡¡¡ÀÏ ] 09 : 00 ~ 18 : 00 ¦¢ [ Á¡½É½Ã°£ ] 12 : 00 ~ 13 : 00
  • Å䡤ÀÏ¿äÀÏ, °øÈÞÀÏÀº ÈÞ¹«ÀÔ´Ï´Ù.
RT-PCR ÇʼöÇ°

Recombinant DNase I (RNase-free)

-

Á¦Á¶»ç Á¦Ç°ÄÚµå Á¦Ç°¸í ¿ë·® °¡°Ý
(ºÎ°¡¼¼º°µµ)
ºñ°í »ç¿ëÀڸŴº¾ó
Takara
2270A
Recombinant DNase I (RNase-free)
°ü·ÃÇмú ±¸¸ÅÇϱâ
1000 U
92,000¿ø 
115,000¿ø
°¡°ÝÇÒÀÎ
09.02 ~ 10.31
2270A_DS.v2107Da.pdf
Takara
2270B
Recombinant DNase I (RNase-free)
°ü·ÃÇмú ±¸¸ÅÇϱâ
5,000 U (2270A x 5)
520,000¿ø 

Á¦Ç°¼³¸í
º» È¿¼Ò´Â ´ÜÀÏ °¡´Ú, ÀÌÁß °¡´Ú DNA ±×¸®°í DNA¿Í RNAÀÇ hybrid¸¦ ¹«ÀÛÀ§·Î ºÐÇØÇÏ¿© 5¡¯-phosphate ¸»´ÜÀ» °¡Áö´Â oligonucleotide¸¦ »ý¼ºÇÏ´Â endodeoxyribonucleaseÀÌ´Ù. Mg2+ Á¸Àç ÇÏ¿¡¼­´Â ÀÌÁß °¡´Ú DNA¿¡ ¹«ÀÛÀ§·Î nickÀ» ¸¸µéÁö¸¸, Mn2+ Á¸Àç ÇÏ¿¡¼­´Â ÀÌÁß °¡´ÚÀ» µ¿½Ã¿¡ Àý´ÜÇÏ¿© DNA¸¦ ´ÜÆíÈ­ÇÑ´Ù.
º» È¿¼Ò´Â È°¼º¿¡´Â ¿µÇâÀÌ ¾øÀ» ¼öÁرîÁö protease ¹× RNase È°¼ºÀ» Á¦°ÅÇÏ¿© Áß¼º pH¿¡¼­ ¾ÈÁ¤¼ºÀÌ Çâ»óµÇ¾î RNA Á¶Á¦½Ã ¾ÈÀüÇÏ°Ô ÀÌ¿ëÇÒ ¼ö ÀÖ´Ù.
º¸Á¸ -20¡É
³óµµ 5 U/§¡
Çü»ó

20 mM

 

Tris-HCl (pH7.5, 4¡É)

50 mM

 

NaCl

0.1 mM

 

CaCl2

50%

 

Glycerol

±â¿ø
Expressed in non-animal host carrying plasmid encodes the gene of bovine pancreas DNase I.
÷ºÎ buffer Á¶¼º (10x)

400 mM

 

Tris-HCl, pH7.5

80 mM

 

MgCl2

50 mM

 

DTT

È°¼ºÀÇ Á¤ÀÇ
25¡É, pH5.0¿¡¼­ ¼Û¾ÆÁö Èä¼± DNA¸¦ ±âÁú·Î 1ºÐ¿¡ OD260¸¦ 0.001 Áõ°¡½ÃÅ°´Â È¿¼Ò È°¼ºÀ» 1 UÀ¸·Î ÇÑ´Ù. (Kunitz unit)
È°¼º ÃøÁ¤¿ë ¹ÝÀÀ¾× Á¶¼º

100 mM

 

Sodium acetate (pH5.0, 25 ¡É)

5 mM

 

MgSO4

40 §¶ / §¢

 

¼Û¾ÆÁö Èä¼± DNA

¿ëµµ
- T7 ¶Ç´Â SP6 Polymerase¸¦ ÀÌ¿ëÇÑ in vitro transcription ÈÄ RNA »ùÇÿ¡¼­ ¹öÆÛ ±³È¯ ¾øÀÌ ÁÖÇü DNA¸¦ Á¦°ÅÇÑ´Ù.
- DNA Polymerase I (Code 2130)°ú °°ÀÌ »ç¿ëÇؼ­ nick translation¿¡ »ç¿ëÇÑ´Ù.
- Mn2+°¡ Á¸ÀçÇÒ ¶§ shot-gun sequencingÀ» À§ÇÑ DNA library Á¦ÀÛ¿¡ »ç¿ëÇÑ´Ù.
- DNA¿Í ´Ü¹éÁúÀÇ »óÈ£ÀÛ¿ëÀ» ºÐ¼®Çϱâ À§ÇÑ foot-printing analysis¿¡ »ç¿ëÇÑ´Ù.
- RT-PCR Àü gDNAÁ¦°Å¿¡ »ç¿ëÇÑ´Ù.
ÀϹÝÀû ¼ºÁú
- ºÐÀÚ·®: ¾à 39,000
- ÃÖÀû pH: pH7.0~pH8.0 (2°¡ ¾çÀÌ¿ÂÀÌ Á¸ÀçÇÒ ¶§ ÃÖ´ë È°¼ºÀ» º¸ÀδÙ.)
- ÀúÇØÁ¦: EDTA
- ¾ÈÁ¤¼º: 75¡É, 10ºÐÀÇ ¿­Ã³¸®¿¡¼­ ºñ°¡¿ªÀûÀ¸·Î ºÒÈ°¼ºÈ­µÈ´Ù.

Keyword :

¼ö½ÄÈ¿¼Ò£¯Nuclease¡¢Topoisomerase
>
Cloned
Exonuclease I
>
´Ü¹éÁú/¹ÙÀÌ·¯½º Á¤Á¦½Ã ÇÙ»êÁ¦°Å
NucleoGone¢â Endonuclease
>
RT-PCR ÇʼöÇ°
Recombinant DNase I (RNase-free)