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È¿À² ³ôÀº TA-cloning¿¡

TaKaRa T-Vector pMD20

-

Á¦Á¶»ç Á¦Ç°ÄÚµå Á¦Ç°¸í ¿ë·® °¡°Ý
(ºÎ°¡¼¼º°µµ)
ºñ°í »ç¿ëÀڸŴº¾ó
Takara
3270
T-Vector pMD20
°ü·ÃÇмú ±¸¸ÅÇϱâ
1 ¥ìg
114,400¿ø 
143,000¿ø
°¡°ÝÇÒÀÎ
09.02 ~ 10.31
3270_DS.v2109Da.pdf
Takara
3271
T-Vector pMD19 (Simple)
°ü·ÃÇмú ±¸¸ÅÇϱâ
1 ¥ìg
114,400¿ø 
143,000¿ø
°¡°ÝÇÒÀÎ
09.02 ~ 10.31
3271_DS.v2109Da.pdf

Á¦Ç°¼³¸í
º» Á¦Ç°Àº Taq DNA polymerase (TaKaRa Taq¢â, TaKaRa Ex Taq¢ç, TaKaRa LA Taq¢ç, SpeedSTAR¢â HS µî)·Î ÁõÆøÇÏ¿© 3¡¯ ¸»´Ü¿¡ dA ´ÜÀÏ¿°±â°¡ ºÎ°¡µÈ PCR »ê¹°À» cloningÇϱâ À§ÇÑ T vectorÀÌ´Ù.
EcoR V È¿¼Ò·Î Àý´ÜµÈ ÈÄ °¢ 3¡¯ ¸»´Ü¿¡ dT ¿°±â°¡ ºÎ°¡µÇ¾î ÀÖ¾î Taq DNA polymerase °è¿­ÀÇ È¿¼Ò·Î ÁõÆøµÈ PCR »ê¹°¿¡ ¹Ù·Î ligationÇÏ¿© blue/white screening ÇÒ ¼ö ÀÖ´Ù. ¶ÇÇÑ T-Vector pMD20Àº MCS site »ó·ù¿¡ SP6 promoter ¼­¿­À» °¡Áö°í ÀÖ¾î, SP6 RNA polymerase¸¦ ÀÌ¿ëÇÏ¿© cloning À¯ÀüÀÚ¿¡ »óº¸ÀûÀÎ single strand RNA¸¦ ÇÕ¼ºÇÒ ¼ö ÀÖ´Ù.
T-Vector pMD19 (Simple)´Â pUC19À» ±âº»À¸·Î ¼³°èµÇ¾î ÀÖÁö¸¸, pUC19 MCS site°¡ ¸ðµÎ Á¦°ÅµÇ¾î ÀÖ¾î cloning ÈÄ PCR »ê¹°¿¡ Á¸ÀçÇÏ´Â Á¦ÇÑ È¿¼Ò »çÀÌÆ®¸¦ ÀÌ¿ëÇÏ´Â °æ¿ì¿¡ À¯¿ëÇÏ´Ù.

[ÁÖÀÇ]
´ÙÄ«¶óÀÇ high fidelity PCR È¿¼ÒÀÎ PrimeSTAR¢ç½Ã¸®Áî ¶Ç´Â TaKaRa Ex Premier¿¡¼­ ÁõÆøµÈ PCR »ê¹°Àº ´ëºÎºÐ blunt end·Î, º» T vector¿¡ ±×´ë·Î cloning ÇÒ ¼ö ¾ø´Ù.
ÀÌ °æ¿ì, PCR »ê¹°ÀÇ 3¡¯ ¸»´Ü¿¡ dA ¿°±â¸¦ ºÎ°¡ÇÏ´Â ½Ã¾àÀÌ Æ÷ÇÔµÈ Mighty TA-cloning Reagent Set for PrimeSTAR¢ç (Code 6019) ¸¦ ÀÌ¿ëÇÏ¿© Æí¸®ÇÏ°Ô TA cloningÀ» ÁøÇàÇÒ ¼ö ÀÖ´Ù.
T-Vector pMD20, T-Vector pMD19 (simple)
³»¿ë (20ȸ)

20 §¡

 

T-Vector pMD20 ¶Ç´Â T-Vector pMD19 (Simple) (50 ng/§¡)

10 §¡

 

Control Insert (50 ng/§¡)*


*3¡¯ ¸»´Ü¿¡ dA ¿°±â¸¦ Æ÷ÇÔÇÏ´Â ¾à 500 bpÀÇ DNA ´ÜÆí
º¸Á¸ -20¡É
°ü·ÃÁ¦Ç°
- PrimeSTAR¢ç µî High fidelity È¿¼Ò¸¦ ÁõÆø»ê¹°ÀÇ TA-cloning Mighty TA-cloning Reagent Set for PrimeSTAR¢ç (Code 6019)
- TaKaRa Ex Taq¢çµî Taq ±â¹Ý polymeraseÀÇ ÁõÆø»ê¹°ÀÇ TA-cloning Mighty TA-cloning Kit (Code 6028)
Âü°íÀÚ·á
- Cloning ½ÇÇè ÀÔ¹®ÀÚ¸¦ À§ÇÑ ¡®Step by Step Cloning °¡À̵塯
- ½ÇÇè ¸ñÀûº°·Î ÃßõÇÏ´Â Cloning Kit

Keyword : T vector,TA cloning,TA-cloning,t cloining,t-cloning,cloning,Ŭ·Î´×,t easy