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Alkaline Phosphatase (E. coli C75) (BAP)

-

Á¦Á¶»ç Á¦Ç°ÄÚµå Á¦Ç°¸í ¿ë·® °¡°Ý
(ºÎ°¡¼¼º°µµ)
ºñ°í »ç¿ëÀڸŴº¾ó
Takara
2120A
Alkaline Phosphatase (E. coli C75)
°ü·ÃÇмú ±¸¸ÅÇϱâ
50 U
68,000¿ø 
85,000¿ø
°¡°ÝÇÒÀÎ
09.02 ~ 10.31
2120A_DS.v2107Da.pdf
Takara
2120B
Alkaline Phosphatase (E. coli C75)
°ü·ÃÇмú ±¸¸ÅÇϱâ
250 U (2120A x 5)
380,000¿ø 

Á¦Ç°¼³¸í
º» È¿¼Ò´Â ¸ðµç Àλê monoester °áÇÕÀ» °¡¼öºÐÇØÇÏÁö¸¸ ´ëºÎºÐÀÇ Àλê diester¿Í Àλê triester °áÇÕÀº ºÐÇØÇÏÁö ¾Ê´Â´Ù. ¶ÇÇÑ ATP µîÀÇ pyrophosphate °áÇÕµµ °¡¼öºÐÇØÇÑ´Ù.
º¸Á¸
-20¡É
³óµµ
0.5 U/§¡
Çü»ó

50 mM

 

Tris-HCl (pH8.0)

100 mM

 

KCl

1 mM

 

MgSO4

50%

 

Glycerol

÷ºÎ buffer ±¸¼º (10x)

500 mM

 

Tris-HCl (pH9.0)

10 mM

 

MgCl2

È°¼ºÀÇ Á¤ÀÇ
p-nitrophenyl phosphate¸¦ ±âÁú·Î ÇÏ¿© 25¡É, pH8.0¿¡¼­ 1 ºÐ°£ 1 ¥ìmolÀÇ p-nitrophenolÀ» À¯¸®½ÃÅ°´Â È¿¼ÒÈ°¼ºÀ» 1 UÀ¸·Î ÇÑ´Ù.
È°¼º ÃøÁ¤¿ë ¹ÝÀÀ¾× Á¶¼º

1 M

 

Tris-HCl (pH8.0)

1 mM

 

p-nitrophenyl phosphate

»ç¿ë»óÀÇ ÁÖÀÇ
º» È¿¼Ò´Â ¸Å¿ì ¾ÈÁ¤¼ºÀÌ ³ôÀº È¿¼Ò·Î, 100¡ÉÀÇ °¡¿­¿¡¼­ ÀϽÃÀûÀ¸·Î ºÒÈ°¼ºÈ­µÇÁö¸¸ ½Ç¿Â¿¡ µÎ¸é È°¼ºÀÌ µÇ»ì¾Æ³ª¹Ç·Î ºÒÈ°¼ºÈ­½ÃÅ°·Á¸é Àû¾îµµ 2ȸÀÇ Æä³î 󸮰¡ ÇÊ¿äÇÏ´Ù.
¿ëµµ
- DNAÀÇ 5¡¯-¸»´Ü Ç¥Áö¸¦ À§ÇÑ Àü ó¸®, ¶Ç´Â ¼±ÇüÈ­µÈ vectorÀÇ self ligation ¹æÁö¸¦ À§ÇÑ vector DNA ´ÜÆíÀÇ Å»ÀλêÈ­ (dephosphorylation)
- RNAÀÇ 5¡¯-¸»´Ü Ç¥Áö¸¦ À§ÇÑ Àü ó¸®, ¶Ç´Â RNA (ȤÀº DNA)ÀÇ T4 RNA Ligase¿¡ ÀÇÇÑ self ligation ¹æÁö¸¦ À§ÇÑ Å»ÀλêÈ­ (dephosphorylation)
±â¿ø
Escherichia coli C 75
ÀϹÝÀû ¼ºÁú
- ºÐÀÚ·®: ¾à 80,000
- Subunit
   »óµ¿ÀÇ polypeptide dimer·Î ÀÌ·ç¾îÁø ±¸»óÀÇ ±Ý¼Ó ´Ü¹éÁú 1 ºÐÀÚ´ç Zn2+ 2ºÐÀÚ¿Í Àλê ÀÌ¿Â 2ºÐÀÚ¸¦ °¡Áö¸ç ¸î °¡ÁöÀÇ ¾ÆÀ̼ÒÀÚÀÓÀÌ ÀÖ´Ù.
- ÃÖÀû pH: pH8 ºÎ±Ù
- È°¼ºÈ­Á¦
   Tris µî ¾ËÄÚ¿Ã È­ÇÕ¹°, Mg2+, Zn2+(10-6 M)
   Zn2+(10-4 M)¿¡¼­´Â ÀúÇØ
- ÀúÇØÁ¦
   ¹«±âÀλê, EDTA, EGTA, cysteine (2x10-4 M), Thioglycolic acid (5x10-3 M), Nitrilotriacetic acid (Zn2+¿¡ ƯÀÌÀûÀΠų·¹ÀÌÆ®Á¦)
°ü·Ã Á¦Ç°
- ½ÇÇè¸ñÀûº°·Î ¼±ÅÃÇÏ´Â Cloning Kit ¼±Åð¡À̵å - ¸µÅ©
DNA Ligation Kit ¡´Mighty Mix¡µ (Code 6023)
Mighty TA-cloning Kit (Code 6028)
Mighty TA-cloning Reagent Set for PrimeSTAR¢ç (Code 6019)
Alkaline Phosphatase (Calf intestine) (Code 2250A)
Alkaline Phosphatase (Shrimp) (Code 2660A)

Keyword :

¼ö½ÄÈ¿¼Ò£¯Alkaline Phosphatase¡¢Polynucleotide Kinase