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Home > ÀüÁ¦Ç°º¸±â > Cloning °ü·Ã > ½Ä¹°¿ë Vectors > Takara Plant ½ÇÇè °ü·ÃÁ¦Ç°

Takara Plant ½ÇÇè °ü·ÃÁ¦Ç°

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½Ä¹° ÇüÁú Àüȯ¿ë¾× binary vector

pRI 201 DNA ½Ã¸®Áî

  • Ri plasmid À¯·¡ÀÇ º¯ÀÌÇü º¹Á¦°³½ÃÁ¡ (Ri ori)À» °¡Áø binary vector
  • High copyÀÇ ColE1 ori¸¦ Æ÷ÇÔÇÏ¿© E. coli ¿¡¼­ º¹Á¦ ¿ëÀÌ
  • °íÈ¿À² Cauliflower mosaic virus (CaMV) 35S promoter žÀç
Code
Á¦Ç°¸í ¿ë·® Ư¡
pRI 201-AN DNA 10 §¶ ½Ö¶±ÀÙ ½Ä¹°¿ë vector
(¾Ö±â Àå´ë, Å丶Åä, ´ã¹è µî)
pRI 201-ON DNA 10 §¶ ¿Ü¶±ÀÙ ½Ä¹°¿ë vector
(º­ µî)
pRI 201-AN-GUS DNA 10 §¶ ¥â-glucuronidase (GUS)
À¯ÀüÀÚ¸¦ Æ÷ÇÔÇÑ
½Ö¶±ÀÙ ½Ä¹°¿ë vector
pRI 201-ON-GUS DNA 10 §¶ ¥â-glucuronidase (GUS)
À¯ÀüÀÚ¸¦ Æ÷ÇÔÇÑ
¿Ü¶±ÀÙ ½Ä¹°¿ë vector

[pRI 201 DNA ½Ã¸®Áî reference]


ÇüÁúÀüȯ ½Ä¹° Á¦ÀÛ¿ë Competent Cell

Agrobacterium tumefaciens LBA4404 Electro-Cells

  • Electorporation ¿ë Agrobacterium competent cells
  • ÇüÁúÀüȯµÈ A. tumefaciens´Â ´Ù¾çÇÑ ½Ä¹°ÀÇ ÇüÁúÀüȯ½ÇÇè¿¡ Àû¿ë °¡´É

Code
Á¦Ç°¸í ¿ë·®
Á¦Ç°±¸¼º
Agrobacterium tumefaciens
LBA4404 Electro-Cells
1 Set - Electro-Cells 40 §¡ ¡¿ 5
- pRI 900 DNA (1 ng/§¡) 10 §¡
- SOC ¹èÁö 1 §¢¡¿10





½Ä¹° DNA/RNA ÃßÃâ½Ã¾à

TaKaRa MiniBEST Plant RNA Extraction Kit (Code 9769A)
TaKaRa MiniBEST Plant Genomic DNA Extraction Kit (Code 9768A)
  • ½Ä¹° DNA ¶Ç´Â RNA ÃßÃâ Àü¿ë spin column kit
  • ½Ä¹°¿¡ ÃÖÀûÈ­µÈ 2Á¾·ùÀÇ lysis buffer Æ÷ÇÔ
    : ÀϹÝÀûÀÎ ½Ä¹° »ùÇÃ, fungi¿ë
    : °ú½Ç, Á¾ÀÚ µî polysaccharide, polyphenol, ÁöÁúÀÌ ¸¹Àº »ùÇÿë




Àü ó¸® - Polysaccharide, polyphenolÀ» °¡Áø ½Ä¹°»ùÇÃÀ» À§ÇÑ
            Fruit-mate¢â for RNA Purification (Code 9192)
ÈÄ Ã³¸® - ÃßÃâµÈ RNA ¿ë¾×³» ´Ù´ç·ù Á¦°Å¸¦ À§ÇÑ
            High-Salt Solution for Precipitation (Plant) (Code 9193)


Direct PCR ¿ë DNA Polymerase

MightyAmp¢â Genotyping Kit (Code R074A)
  • PCR ÁõÆøÀ» ÀÌ¿ëÇÑ genotyping¿¡ ÃÖÀû
  • ¸¶¿ì½º²¿¸®, ½Ä¹°ÀÙ, ½Ò µî¿¡¼­ °£´ÜÇÏ°Ô DNA ÃßÃâ ÈÄ PCR ÁõÆø
  • °£ÀÌ ÃßÃâ½Ã¾à, Àü¿ë DNA polymerase, loading dye±îÁö ¸ðµÎ Æ÷ÇÔ