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Cloning °ü·Ã
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½Ä¹°¿ë Vectors
> pRI 201 DNA ½Ã¸®Áî
½Ä¹° ÇüÁúÀüȯ¿ë °í¹ßÇö Vecto£¨New version£©
pRI 201 DNA ½Ã¸®Áî
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Á¦Ç°ÄÚµå
Á¦Ç°¸í
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(ºÎ°¡¼¼º°µµ)
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Takara
3264
pRI 201-AN DNA
10 ¥ìg
537,600¿ø
672,000¿ø
09.02 ~ 10.31
,
Takara
3265
pRI 201-ON DNA
10 ¥ìg
537,600¿ø
672,000¿ø
09.02 ~ 10.31
,
Takara
3266
pRI 201-AN-GUS DNA
10 ¥ìg
484,000¿ø
605,000¿ø
09.02 ~ 10.31
Takara
3267
pRI 201-ON-GUS DNA
10 ¥ìg
484,000¿ø
605,000¿ø
09.02 ~ 10.31
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* Takara Plant ½ÇÇè °ü·ÃÁ¦Ç° overview (Ŭ¸¯)
Á¦Ç°¼³¸í
pRI 201 DNA½Ã¸®Áî´Â pRI 101½Ã¸®Áî (Code 3262/3263)º¸´Ù ´õ ³ôÀº È¿À²·Î ÇüÁúÀüȯ ½Ä¹°¿¡¼ ¿Ü·¡À¯ÀüÀÚ¸¦ ¹ßÇö½ÃÅ°±â À§ÇØ °³·®µÈ vectorÀÌ´Ù. Cauliflower mosaic virus (CaMV)ÀÇ 35S protomer ÇÏ·ù¿¡ ADH (Alcohol Dehydrogenase) À¯ÀüÀÚ À¯·¡ÀÇ5¡¯ ºñ¹ø¿ª ¿µ¿ª (5¡¯-UTR) (translation enhancer ¿µ¿ª)¸¦ žÀçÇÏ¿´´Ù. pRI 101½Ã¸®Áî DNA¸¦ ±âº»À¸·Î
1) Á¾·¡ÀÇNOS (Nopaline Synthase) À¯ÀüÀÚ À¯·¡ terminator¸¦ HSP (Heat Shock Protein) À¯ÀüÀÚ À¯·¡ terminator·Î ¹Ù²Ù´Â °ÍÀ¸·Î, pRI 101½Ã¸®Áî¿¡ ºñÇØ ½Ä¹°Ã¼¿¡¼ ¸ñÀû À¯ÀüÀÚ »ê¹°ÀÇ °í¹ßÇöÀ» ±â´ëÇÒ ¼ö ÀÖ´Ù
2). ¶Ç, HSP terminator ÇÏ·ùÀÇ cloning site (MCS2)¿¡ ´Ù¸¥ À¯ÀüÀÚ¸¦ Æ÷ÇÔÇÑ ¹ßÇö Ä«¼ÂÆ® (promoter£«enhancer£«¸ñÀû À¯ÀüÀÚ+terminator)¸¦ µµÀÔÇÏ¿© ÇϳªÀÇ º¤ÅÍ·Î º¹¼öÀÇ À¯ÀüÀÚ¸¦ ÇüÁú ÀüȯÇÏ´Â °ÍÀÌ °¡´ÉÇÏ´Ù.
pRI 201½Ã¸®Áî¿¡´Â ¾Ö±âÀå´ë ADHÀ¯·¡ 5¡¯-UTR (AtADH 5¡¯-UTR)À» žÀçÇÏ¿´´Ù. pRI 201-AN DNA¿Í º ADH À¯·¡ 5¡¯-UTR (OsADH 5¡¯-UTR)À» žÀçÇÏ¿´´Ù. pRI 201-ON DNAÀÇ2Á¾·ù°¡ ÀÖ¾î ½ÖÀÚ¿± ½Ä¹°¿¡´Â pRI 201-AN DNA, ´ÜÀÚ¿± ½Ä¹°¿¡´Â pRI 201-ON DNA°¡ ÀûÇÕÇÏ´Ù. ¶Ç, ÇüÁú Àüȯ½ÃÀÇ positive control·Î¼ ³Î¸® ÀÌ¿ëµÇ´Â GUS (¥â-glucuronidase) À¯ÀüÀÚ¸¦ °¢°¢ÀÇ º¤ÅÍ¿¡ ³ÖÀº control vector (pRI 201-AN-GUS DNA,pRI 201-ON-GUS DNA)µµ ÆǸÅÇÏ°í ÀÖ´Ù.
º» Á¦Ç°Àº ½Ä¹° ÇüÁú Àüȯ¿ë binary vector pRI 910 DNA (Code 3261)¸¦ ±âº»À¸·Î ÇÑ binary vectorÀ̸ç, Rhizobium rhizogenesÀÇRiÇÙ¿Ü À¯ÀüÀÚ À¯·¡ÀÇ º¯ÀÌÇü º¹Á¦ ±âÁ¡ (Ri ori)À» °¡Áö°í ÀÖ´Ù3). pUC°èÀÇ ÇÙ ¿Ü À¯ÀüÀÚ¿Í °°Àº º¹Á¦ ±âÁ¡ (ColE1 ori)À» °¡Áö°í ÀÖ¾î, ´ëÀå±Õ ³»¿¡¼ ³ôÀº copy¼ö¸¦ À¯ÁöÇÏ¸ç ¶ÇÇÑ, cloning site°¡ ½Ä¹°ÀÇ selection marker¿¡ ºñÇØ T-DNAÀÇRight Border (RB) Ãø¿¡ ¹èÄ¡µÇ¾î ÀÖ¾î ¸ñÀû À¯ÀüÀÚ°¡ °á¿©µÇ´Â ÀÏ ¾øÀÌ ¾ÈÁ¤ÀûÀ¸·Î ½Ä¹° ¿°»öü¿¡ À¯ÀüÀÚ »ðÀÔÀÌ °¡´ÉÇÏ°Ô µÇ´Â µî, pRI 910 DNA°¡ °¡Áö´Â Ư¡µµ º¸°ü À¯ÁöÇÏ°í ÀÖ´Ù.
¡Øº» º¤ÅÍ´Â, ±¹¸³´ëÇÐ ¹ýÀÎ ³ª¶ó ÷´Ü °úÇбâ¼ú ´ëÇпø ´ëÇкÎÅÍ ±â¼ú ¹× ½Ã·áÀÇ Á¦°øÀ» ¹Þ°í, ´ÙÄ«¶ó ¹ÙÀÌ¿À(ÁÖ)°¡ Á¦Ç°ÈÇß½À´Ï´Ù.
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Binary vector¹ý¿¡ ÀÇÇØ Rhizobium (Agrobacterium)À» ¸Å°³·Î ½Ä¹° ÇüÁú ÀüȯÀ» À§ÇÑ vector. ¾Ö±âÀå´ë, Å丶Åä, ´ã¹è µîÀÇ ½ÖÀÚ¿± ½Ä¹° (pRI 201-AN DNA, pRI 201-AN-GUS DNA¸¦ »ç¿ë) ¶Ç´Â º µîÀÇ ´ÜÀÚ¿± ½Ä¹° (pRI 201-ON DNA, pRI 201-ON-GUS DNA¸¦ »ç¿ë)ÀÇ ÇüÁú Àüȯ ¹× ¿Ü·¡ À¯ÀüÀÚÀÇ °í¹ßÇöÀ» ½ÇÇö.
³»¿ë (³óµµ: °¢ 0.5 ¥ìg/¥ìl)
pRI 201-AN DNA (TaKaRa Code 3264)
10 ¥ìg
pRI 201-ON DNA (TaKaRa Code 3265)
10 ¥ìg
pRI 201-AN-GUS DNA (TaKaRa Code 3266)
10 ¥ìg
pRI 201-ON-GUS DNA (TaKaRa Code3267)
10 ¥ìg
º¸Á¸ -20¡É
±æÀÌ
pRI 201-AN DNA
10,432 bp
pRI 201-ON DNA
10,475 bp
pRI 201-AN-GUS DNA
12,220 bp
pRI 201-ON-GUS DNA
12,263 bp
pRI 201 DNA ½Ã¸®ÁîÀÇ ±¸Á¶
ColE1 ori£º ´ëÀå±Õ À¯·¡ º¹Á¦ ±âÁ¡ (´ëÀå±Õ À¯·¡)
Ri ori£º
Rhizobium
À¯·¡ º¹Á¦ ±âÁ¡ (
Rhizobium rhizogenes
(
Agrobacterium rhizogenes
) À¯·¡)
RB, LB£º ½Ä¹°¿°»öü¿¡ »ðÀԵǴ T-DNA ÀÇ border ¼¿ (
Rhizobium radiobacter
(
Agrobaceterium tumefaciens
) À¯·¡)
NOS promoter, NOS terminator£º ½Ä¹°Ã¼³»ÀÇ À¯ÀüÀÚ ¹ßÇöÀ» À§ÇÑ promoter, terminator (
Rhizobium radiobacter
(
Agrobaceterium tumefaciens
) À¯·¡)
HSP terminator£º ½Ä¹°·ÎÀÇ À¯ÀüÀÚ ¹ßÇöÀ» À§ÇÑ terminator (
Arabidopsis thaliana
À¯·¡)
GUS£º ¥â-glucuronidase À¯ÀüÀÚ (´ëÀå±Õ À¯·¡)
NPT II£º ½Ä¹°Ã¼³»ÀÇ selection marker À¯ÀüÀÚ (´ëÀå±Õ À¯·¡)
AtADH 5¡¯-UTR, OsADH 5¡¯-UTR£º translation enhancer ¿µ¿ª (
Arabidopsis thaliana
¶Ç´Â
Oryza sativa
À¯·¡)
35S promoter£º ½Ä¹°Ã¼³»ÀÇ À¯ÀüÀÚ ¹ßÇöÀ» À§ÇÑ promoter (CaMV À¯·¡)
NPT III£º ´ëÀå±Õ ¹×
Rhizobium
(
Agrobacterium
)ÀÇ selection marker À¯ÀüÀÚ (kanamycin ³»¼º) (
Streptococcus faecalis
À¯·¡)
Keyword :
½Ä¹°¿ë Vectors
Takara Plant ½ÇÇè °ü·ÃÁ¦Ç°
½Ä¹° ÇüÁúÀüȯÀ» À§ÇÑ À¯ÀüÀÚ µµÀÔ¿¡
Agrobacterium tumefaciens LBA4404 Electro-Cells
½Ä¹° ÇüÁúÀüȯ¿ë °í¹ßÇö Vector
pRI 101 DNA ½Ã¸®Áî
½Ä¹° ÇüÁúÀüȯ¿ë °í¹ßÇö Vecto£¨New version£©
pRI 201 DNA ½Ã¸®Áî
½Ä¹° ÇüÁúÀüȯ¿ë Binary Vector
pRI 909/910 DNA