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Alkaline Phosphatase (Calf intestine) (CIAP)

-

Á¦Á¶»ç Á¦Ç°ÄÚµå Á¦Ç°¸í ¿ë·® °¡°Ý
(ºÎ°¡¼¼º°µµ)
ºñ°í »ç¿ëÀڸŴº¾ó
Takara
2250A
Alkaline Phosphatase (Calf intestine)
°ü·ÃÇмú ±¸¸ÅÇϱâ
1,000 U
72,000¿ø 
90,000¿ø
°¡°ÝÇÒÀÎ
09.02 ~ 10.31
2250A_DS.v2107Da.pdf
Takara
2250B
Alkaline Phosphatase (Calf intestine)
°ü·ÃÇмú ±¸¸ÅÇϱâ
5,000 U (2250A x 5)
405,000¿ø 

Á¦Ç°¼³¸í
º» È¿¼Ò´Â ´ëÀå±Õ À¯·¡ÀÇ È¿¼Ò¿Í ¸¶Âù°¡Áö·Î ´ëºÎºÐÀÇ Àλê monoester °áÇÕÀ» ºÐÇØÇÏÁö¸¸, Àλê diester¿Í Àλê triester °áÇÕÀº ºÐÇØÇÏÁö ¾Ê´Â´Ù. ¶ÇÇÑ ATP µîÀÇ pyrophosphate °áÇÕÀÇ ºÐÇؼӵµ°¡ ´À¸®´Ù.
º¸Á¸
-20¡É
³óµµ
30 U/§¡
Çü»ó

10 mM

 

Tris-HCl (pH8.0)

50 mM

 

KCl

1 mM

 

MgCl2

0.1 mM

 

ZnCl2

50%

 

Glycerol

÷ºÎ buffer ±¸¼º (10x)

500 mM

 

Tris-HCl (pH9.0)

10 mM

 

MgCl2

È°¼ºÀÇ Á¤ÀÇ
37¡É, pH9.8¿¡¼­ 1ºÐ°£ 1 ¥ìmolÀÇ p-nitrophenolÀ» À¯¸®½ÃÅ°´Â È¿¼ÒÈ°¼ºÀ» 1 UÀ¸·Î ÇÑ´Ù.
È°¼º ÃøÁ¤¿ë ¹ÝÀÀ¾× Á¶¼º

1 M

 

Diethanolamine (pH9.8)

0.5 mM

 

MgCl2

15 mM

 

p-nitrophenyl phosphate

»ç¿ë»óÀÇ ÁÖÀÇ
º» È¿¼Ò´Â º¸Á¸ Á¶°Ç¿¡¼­ ¸Å¿ì ¾ÈÁ¤ÇÏÁö¸¸ chelate°¡ Á¸ÀçÇÒ ¶§ 65 ¡É, 30ºÐÀÇ ¿­Ã³¸®·Î 99% ÀÌ»óÀÇ È°¼ºÀÌ ºñ°¡¿ªÀûÀ¸·Î ½ÇÈ°µÈ´Ù.
´Ü, »ç¿ë Á¶°Ç¿¡ µû¶ó¼­´Â ÃæºÐÇÏÁö ¾ÊÀ» ¼ö ÀÖÀ¸¹Ç·Î, gel extraction ¶Ç´Â phenol-chloroform Á¤Á¦¸¦ ÅëÇØ ¿ÏÀüÈ÷ ºÒÈ°¼ºÈ­½ÃÅ°´Â °ÍÀ» ÃßõÇÑ´Ù.
¿ëµµ
- DNAÀÇ 5¡¯-¸»´Ü Ç¥Áö¸¦ À§ÇÑ Àü ó¸®, ¶Ç´Â ¼±ÇüÈ­µÈ vectorÀÇ self ligation ¹æÁö¸¦ À§ÇÑ Å»ÀλêÈ­(dephosphorylation)
- ÆòÈ°¸»´Ü (blunt end) ¶Ç´Â 3¡¯-µ¹Ã⸻´Ü (3¡¯-overhang)ÀÇ Å»ÀλêÈ­¿¡´Â Alkaline Phosphatase (E. coli C75) (Code 2120A)À» ÀÌ¿ëÇÑ °í¿Â (55¡É~60¡É)¿¡¼­ÀÇ ¹ÝÀÀÀÌ ¹Ù¶÷Á÷ÇÏ´Ù.
±â¿ø
Yeast carrying the plasmid which encodes the gene of calf intestine alkaline phosphatase
ÀϹÝÀû ¼ºÁú
- ºÐÀÚ·®: ¾à 100,000
- Subunit: ºÐÀÚ·® ¾à 50,000ÀÇ Zn2+À» Æ÷ÇÔÇÑ 2°³ÀÇ ´ç´Ü¹éÁú
- ÃÖÀû pH
   pH10 ºÎ±Ù (³ôÀº ±âÁú ³óµµ)
   pH8 ºÎ±Ù (³·Àº ±âÁú ³óµµ)
- È°¼ºÈ­Á¦
   Trisµî ¾ËÄÚ¿Ã È­ÇÕ¹°, Mg2+
- ¾ÈÁ¤¼º
   ¸Å¿ì ¾ÈÁ¤
    65¡É, 30ºÐÀÇ ¿­Ã³¸®·Î °¡¿ªÀûÀ¸·Î ºÒÈ°¼ºÈ­ÇÑ´Ù. ¿ÏÀüÈ÷ ºÒÈ°¼ºÈ­½ÃÅ°±â À§Çؼ­´Â Æä³î 󸮰¡ ÇÊ¿äÇÏ´Ù.
°ü·ÃÁ¦Ç°
- ½ÇÇè¸ñÀûº°·Î ¼±ÅÃÇÏ´Â Cloning Kit ¼±Åð¡À̵å - ¸µÅ©
DNA Ligation Kit ¡´Mighty Mix¡µ (Code 6023)
Mighty TA-cloning Kit (Code 6028)
Mighty TA-cloning Reagent Set for PrimeSTAR¢ç (Code 6019)
Alkaline Phosphatase (E. coli C75) (Code 2120A)
Alkaline Phosphatase (Shrimp) (Code 2660A)

Keyword : cloning,CIAP,CIP,Calf intestine

¼ö½ÄÈ¿¼Ò£¯Alkaline Phosphatase¡¢Polynucleotide Kinase