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Cloning °ü·Ã
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È¿¸ð ¡¤ °è»ó±Õ ¿ë Vectors
> pAUR101 DNA
Chromosomal Integrating È¿¸ð Suttle Vector
pAUR101 DNA
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Takara
3600
pAUR101 DNA (È¿¸ð ¿°»öü »ðÀÔÇü vector)
20 ¥ìg
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pAUR101 DNA
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pUC118/119 type vector
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¼ö½ÄÈ¿¼Ò¿¡ ´ëÇÏ¿©
¼ö½ÄÈ¿¼Ò ¼øµµ À϶÷
¼ö½ÄÈ¿¼Òº° ¿ëµµ
¼ö½ÄÈ¿¼Ò£¯Ligase
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±× ¿Ü (À¯Àü°øÇÐ °ü·Ã)
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Proteinase K
Westase (È¿¸ð ¼¼Æ÷º® ..
Yatalase
Yatalase -Plus-
³óµµ : 1 mg/ §¢
º¸Á¸ : -20¡É
GenBank
Accession No. AB012282
Á¦Ç°¼³¸í
º» Á¦Ç°Àº
Saccharomyces cerevisiae
ÀÇ ¿°»öü µµÀÔÇü È¿¸ð shuttle vectorÀÌ´Ù. pAUR101Àº Aureobasidin A (AbA) ³»¼ºÀ¯ÀüÀÚ AUR1-C¸¦ ¼±Åà ¸¶Ä¿·Î ÇÏ´Â vector·Î ÇüÁúÀüȯü´Â AbA ³»¼ºÀÌ´Ù. Ŭ·Î´× »çÀÌÆ®·Î 7Á¾·ùÀÇ Á¦ÇÑÈ¿¼Ò Àý´ÜºÎÀ§ (
Sac
I,
Kpn
I,
Sma
I,
Xba
I,
Sal
I,
Sse
8387 I,
Sph
I)¸¦ °®´Â´Ù. vector´Â È¿¸ð ³»¿¡¼´Â ÀÚÀ²ÀûÀ¸·Î º¹Á¦ÇÏÁö ¾Ê°í ¿°»öü¿¡ µµÀÔµÈ »óÅ¿¡¼¸¸ ¾ÈÁ¤ÀûÀ¸·Î º¸À¯¡¤ À¯ÁöµÈ´Ù. AUR1-C À¯ÀüÀÚ ³»ÀÇ Á¦ÇÑÈ¿¼Ò ºÎÀ§ (
Bst
P I,
Eco
O65 I,
Bsi
W I,
Stu
I) Áß Çѱºµ¥ Àý´ÜÇÑ ¼±ÇüÈµÈ plasmid·Î ÇüÁúÀüȯÇÔÀ¸·Î½á ³ôÀº È¿À²·Î È¿¸ð¿°»öü (aur1 À¯ÀüÀÚ ºÎÀ§)¿¡ µµÀԵȴÙ.
±æÀÌ
6,687 bp
¿ëµµ
Ç×»ý¹°Áú Aureobasidin A¸¦ ÀÌ¿ëÇÑ È¿¸ðÀÇ ÇüÁúÀüȯÀ» À§ÇÑ º¤ÅÍ (¿°»öü µµÀÔÇü shuttle º¤ÅÍ)
License Notice : [M11]
pAUR101 DNAÀÇ ±¸Á¶
±×¸² pAUR101 DNA Á¦ÇÑÈ¿¼Ò ¸Ê ¹× Ŭ·Î´× »çÀÌÆ®
AUR1-C
S. cerevisiae
ÀÇ AbA ³»¼º À¯ÀüÀÚ
Amp
r
E. coli
¿¡¼ ¼±Åà ¸¶Ä¿
Ori
E. coli
¿¡¼ÀÇ º¹Á¦ ±âÁ¡ (pUC119 À¯·¡)
Keyword :
È¿¸ð ¡¤ °è»ó±Õ ¿ë Vectors
Chromosomal Integrating È¿¸ð Suttle Vector
pAUR101 DNA
È¿¸ð ÀÚÀ²º¹Á¦Çü Suttle Vector
pAUR112 DNA
È¿¸ð·Î ´Ü¹éÁú ¹ßÇöÇü Shuttle Vector
pAUR123 DNA
Ãâ¾Æ È¿¸ð ¿ë Marker Á¦°ÅÇü Vector
pAUR135 DNA
Schizo. pombe ¿ë ´Ü¹éÁú ¹ßÇöÇü Shuttle Vecto..
pAUR224 DNA
Aspergillus ¿ë Shuttle Vector
pAUR316 DNA
Chromosomal Integrating Çü Shuttle Vector
pPTR I DNA
ÀÚÀ² º¹Á¦Çü Shuttle Vector
pPTR II DNA