닫기
TaKaRa
로그인  |   회원가입  |   ID/PW 찾기  
제품명/제품코드
키워드검색
검색
Home > 전제품보기 > Cloning 관련 > In-Fusion Cloning > In-Fusion® Ready Products

In-Fusion® Ready Products

-

제조사 제품코드 제품명 용량 가격
(부가세별도)
비고 사용자매뉴얼
Clontech
632500
pAcGFP1-C In-Fusion® Ready Vector
관련학술 관심상품등록 구매하기 라이선스 
1 μg
777,000원  제조사 페이지로 바로가기 In-Fusion Ready Vector Cloning Protocol-At-A-Glance (PT3865-2)_070213.pdf
Clontech
632501
pAcGFP1-N In-Fusion® Ready Vector
관련학술 관심상품등록 구매하기 라이선스 
1 μg
777,000원  제조사 페이지로 바로가기 In-Fusion Ready Vector Cloning Protocol-At-A-Glance (PT3865-2)_070213.pdf
Clontech
632498
pDsRed-Monomer-N In-Fusion® Ready Vector
관련학술 관심상품등록 구매하기 라이선스 
1 μg
777,000원  제조사 페이지로 바로가기 In-Fusion Ready Vector Cloning Protocol-At-A-Glance (PT3865-2)_070213.pdf
Clontech
632499
pDsRed-Monomer-C In-Fusion® Ready Vector
관련학술 관심상품등록 구매하기 라이선스 
1 μg
777,000원  제조사 페이지로 바로가기 In-Fusion Ready Vector Cloning Protocol-At-A-Glance (PT3865-2)_070213.pdf


pAcGFP1 Vector Information

Restriction map and multiple cloning site (MCS) of pAcGFP1.
Unique restriction sites are in bold. NOTE: The Xba I and Bcl I sites are methylated in the DNA provided by Clontech Laboratories, Inc. If you wish to digest the vector with these enzymes, you will need to transform the vector into a dam - host and make fresh DNA.

Location of features
  • lac promoter: 95 - 178
    CAP binding site: 111 - 124
    - 35 region: 143 - 148; - 10 region: 167 - 172
    Transcription start point: 179
    lac operator: 179 - 199
  • lacZ - ECFP fusion protein expressed in E. coli
    Ribosome binding site: 206 - 209
    Start codon (ATG): 217 - 219; stop codon: 1006 - 1008
  • 5' multiple cloning site: 234 - 281
  • Aequorea coerulescens Green fluorescent protein (AcGFP1) gene
    Kozak consensus translation initiation site: 282 - 292
    Start codon (ATG): 289 - 291; stop codon: 1006 - 1008
    Insertion of Val at position 2: 292 - 294
    Last amino acid: 1003 - 1005
  • 3' Multiple Cloning Site: 1009 - 1108 Ampicillin resistance gene:
    Promoter: - 35 region: 1484 - 1450, - 10 region: 1507 - 1512
    Ribosome binding site: 1542 - 1546
    β-lactamase coding sequences:
    start codon (ATG): 1554 - 1556; stop codon: 2412 - 2414
    β-lactamase signal peptide: 1554 - 1622
    β-lactamase mature protein: 1623 - 2411

Keyword :
-
In-Fusion Cloning
> In-Fusion® Cloning 실제 사용후기
> In-Fusion® Cloning 실제 사용자 후기 2탄
> In-Fusion® HD Cloning FAQ
> Online In-Fusion Tools
Premix화! 시간단축! 효율성 향상!
> In-Fusion® HD Cloning Kit
> In-Fusion® HD Cloning Plus
> In-Fusion® HD EcoDry Cloning Kit
> In-Fusion® HD EcoDry Cloning Plus
> In-Fusion® Ready Products
-