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Mung Bean Nuclease

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Á¦Á¶»ç Á¦Ç°ÄÚµå Á¦Ç°¸í ¿ë·® °¡°Ý
(ºÎ°¡¼¼º°µµ)
ºñ°í »ç¿ëÀڸŴº¾ó
Takara
2420A
Mung Bean Nuclease
°ü·ÃÇмú °ü½É»óǰµî·Ï ±¸¸ÅÇϱâ
2,000 U
96,000¿ø  2420A_DS.v1901Da.pdf
Takara
2420B
Mung Bean Nuclease
°ü·ÃÇмú °ü½É»óǰµî·Ï ±¸¸ÅÇϱâ
10,000 U
432,000¿ø 

³óµµ 10~50 U/§¡
Çü»ó
10 mM Tris-HCl (pH7.5)
0.1 mM (CH3COO)2 Zn
50% Glycerol
º¸Á¸ -20¡É
÷ºÎ ¹öÆÛ Á¶¼º(10¡¿)
300 mM CH3COONa (pH5.0)
1,000 mM NaCl
10 mM (CH3COO)2Zn
50% Glycerol
±â¿ø
Mung bean sprouts
Á¦Ç°¼³¸í
º» È¿¼Ò´Â ´ÜÀϰ¡´Ú ƯÀÌÀûÀÎ endonuclease·Î½á 5¡¯-P ¸»´ÜÀ» °®´Â mono ¶Ç´Â oligonucleotide¸¦ »ý¼ºÇÑ´Ù. °ú·®(1,000¹è)ÀÇ È¿¼Ò¸¦ »ç¿ëÇϸé, oligomerµµ ÀüºÎ mononucleotide°¡ µÈ´Ù. ÀÌÁß°¡´Ú DNA¿Í RNA, ¶Ç´Â DNA-RNAÀÇ hybrid¿¡ ´ëÇØ¼­´Â ´Ù·®ÀÇ È¿¼Ò¸¦ »ç¿ëÇÏÁö ¾ÊÀ¸¸é ºÐÇØµÇÁö ¾Ê´Â´Ù. ÀÌ °æ¿ì´Â AT-rich ¿µ¿ªÀ» ¼±ÅÃÀûÀ¸·Î ºÐÇØÇÑ´Ù. A¡é pN, T¡é pN site¸¦ ¼±È£ÇØ, ƯÈ÷ A¡é pN site´Â 100% ºÐÇØÇÏÁö¸¸ C¡é pC, C¡é pG site´Â ºÐÇØÇÏ±â ¾î·Æ´Ù.
Ȱ¼ºÀÇ Á¤ÀÇ
¿­º¯¼º ¼Û¾ÆÁö Èä¼± DNA¸¦ ±âÁú·Î ÇÏ¿© 37¡É, pH5.0¿¡¼­ 1ºÐ µ¿¾È 1 §¶ÀÇ »ê°¡¿ë¼º ºÐÇØ¹°À» »ý¼ºÇÏ´Â È¿¼ÒȰ¼ºÀ» 1 UÀ¸·Î ÇÑ´Ù.
Ȱ¼º ÃøÁ¤¿ë ¹ÝÀÀ¾× Á¶¼º
30 mM CH3COONa (pH5.0)
100 mM NaCl
1 mM (CH3COO)2Zn
10% Glycerol
0.5 mg/ml ±âÁú DNA
¼øµµ
30 UÀÇ º» È¿¼Ò¿Í 1 §¶ÀÇ ¥ëDNA-Hind III ºÐÇØ¹°À» 37¡É, 10ºÐ°£ ¹ÝÀÀÇÏ¿©µµ DNAÀÇ Àü±â¿µµ¿ ÆÐÅÏ¿¡ º¯È­°¡ ¾ø´Ù.
»ç¿ë»óÀÇ ÁÖÀÇ
- ÀÌÁß°¡´Ú ½Äº°´É·ÂÀº ¿°±â¼­¿­¿¡ ÀÇÁ¸Çϰí ÀÖ¾î, Àý´ÜÁ¡À¸·Î A¡é pN°ú T(U)¡é pN À» ¼±È£ÇÑ´Ù. ƯÈ÷ A¡é pA¸¦ ¿ÏÀüÈ÷ ºÐÇØÇÏÁö¸¸ G¿Í CÀÇ ¼­¿­À» °ÅÀÇ ºÐÇØÇÏÁö ¾Ê´Â´Ù.
- S1 Nuclease¿Í ´Þ¸®, nickÀÇ ¹Ý´ëÂÊ °¡´ÚÀº Àý´ÜÇÏÁö ¾Ê´Â´Ù.
¿ëµµ
- HybridizationÀÇ mapping (S1 mapping) ƯÈ÷ º» È¿¼Ò´Â S1 nuclease¿Í ºñ±³ÇÏ¿© °ú¼ÒÈ­ (nibbling)ÇÏÁö ¾Ê¾Æ Á¤È®ÇÑ ¹êµå¸¦ Çü¼ºÇÑ´Ù.
- ÀÌÁß°¡´Ú DNA¸»´ÜÀÇ ÆòȰ ¸»´ÜÈ­(´Ü, ÆòȰȭÀÇ È¿À²Àº ¿°±â¼­¿­¿¡ ÀÇÁ¸ÇϹǷΠÁÖÀÇÇÒ °Í)

Keyword :
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¼ö½ÄÈ¿¼Ò£¯Nuclease¡¢Topoisomerase
> NucleaseÀÇ Æ¯¼º ºñ±³
> BAL 31 Nuclease
> DNA Topoisomerase I
Cloned
> Exonuclease I
Cloned
> Exonuclease III
> Micrococcal Nuclease
> Mung Bean Nuclease
> Recombinant DNase I (RNase-free)
Cloned
> Ribonuclease H (RNase H)
> S1 Nuclease
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