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Home
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ÀüÁ¦Ç°º¸±â
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Cloning °ü·Ã
>
Vector (Cloning ¿ë)
> pUC118/119 type vector
pUC118/119 type vector
Á¦Á¶»ç
Á¦Ç°ÄÚµå
Á¦Ç°¸í
¿ë·®
°¡°Ý
(ºÎ°¡¼¼º°µµ)
ºñ°í
»ç¿ëÀڸŴº¾ó
Takara
3318
pUC118 DNA
25 ¥ìg
(0.5 OD)
83,000¿ø
Takara
3319
pUC119 DNA
25 ¥ìg
(0.5 OD)
83,000¿ø
Takara
3320
pUC118 EcoR I/BAP
5 ¥ìg
(0.1 OD)
100,000¿ø
Takara
3321
pUC118 BamH I/BAP
5 ¥ìg
(0.1 OD)
100,000¿ø
Takara
3322
pUC118 Hinc II/BAP
5 ¥ìg
(0.1 OD)
100,000¿ø
Takara
3323
pUC118 Pst I/BAP
5 ¥ìg
(0.1 OD)
100,000¿ø
Takara
3324
pUC118 Hind III/BAP
5 ¥ìg
(0.1 OD)
100,000¿ø
Takara
3328
pTV118N DNA
25 ¥ìg
(0.5 OD)
100,000¿ø
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pBR322 DNA
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pUC118/119 type vector
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¼ö½ÄÈ¿¼Ò¿¡ ´ëÇÏ¿©
¼ö½ÄÈ¿¼Ò ¼øµµ À϶÷
¼ö½ÄÈ¿¼Òº° ¿ëµµ
¼ö½ÄÈ¿¼Ò£¯Ligase
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¼ö½ÄÈ¿¼Ò£¯Alkaline Phospha..
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¹èÁö ¡¤ Buffer (ºÐ¸»)
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IPTG£¨dioxane free£©£¨..
X-Gal£¨5-Bromo-4-Chlor..
±× ¿Ü (À¯Àü°øÇÐ °ü·Ã)
DNA-OFF¢ç
Proteinase K
Westase (È¿¸ð ¼¼Æ÷º® ..
Yatalase
Yatalase -Plus-
³óµµ
pUC118, pUC119, pTV118N :
250~1,000 §¶/§¢
pUC118 BAPó¸® DNA :
50~250 §¶/§¢
º¸Á¸ -20¡É
GenBank
Entry Name
Accession No.
pUC118
U07649
U07649
pUC119
U07650
U07650
ÁÖ) Gen Bank¿¡ µî·ÏµÇ¾î ÀÖ´Â pUC118 ÀÇ ¼¿Àº 2540¹øÀÌ CÀÌÁö¸¸, ´ç»ç¿¡¼ ÆǸÅÇÏ´Â pUC118 º¤ÅÍ´Â 2540¹øÀÌ TÀÌ´Ù. ¶ÇÇÑ GenBank¿¡ µî·ÏµÈ pUC119ÀÇ ¼¿Àº 597¹ø, 852¹ø, 901¹ø ¹× 2540¹øÀÌ CÀÌÁö¸¸, ´ç»ç¿¡¼ ÆǸÅÇÏ´Â º¤ÅÍ´Â À̵é ÀÚ¸®°¡ ¸ðµÎ T ÀÌ´Ù.
DNA ±æÀÌ(MessingÀÇ ±¸Ãà¹ýÀ¸·Î °è»ê)
pUC118/pUC119 :
3,162 bp
pTV118N :
3,163 bp
Á¦Ç°¼³¸í
pUC118/pUC119 vector´Â pUC18/pUC19 ³»¿¡ M13 phage DNAÀÇ intergenic region (IG)À» »ðÀÔÇÑ plasmidÀÌ´Ù. Helper phage M13KO7ÀÇ °¨¿°¿¡ ÀÇÇØ pUC118/pUC119´Â ssDNA°¡ µÇ¾î ¿ì¼±ÀûÀ¸·Î phage ÀÔÀÚ ³»¿¡ packagingµÇ¾î ±Õü ¹ÛÀ¸·Î ¹æÃâµÈ´Ù. ÀÌ ½Ã½ºÅÛÀ» »ç¿ëÇÏ¿© Ä¿´Ù¶õ DNA ´ÜÆíµµ deletion ¾øÀÌ ¾ÈÁ¤ÇÏ°Ô ssDNA¸¦ ¾òÀ» ¼ö ÀÖ´Ù.
¶ÇÇÑ, pUC118Àº pUC18°ú, pUC119´Â pUC19¿Í °¢°¢ µ¿ÀÏÇÑ cloning site¸¦ °¡Áö°í ÀÖ¾î IPTG¿Í X-GalÀ» Æ÷ÇÔÇÏ´Â Ç÷¹ÀÌÆ®¿¡¼ ¿Ü·¡ DNAÀÇ µµÀÔ À¯¹«¸¦ ¿ëÀÌÇÏ°Ô ÆǺ°ÇÒ ¼ö ÀÖ´Ù. Á¦ÇÑÈ¿¼Ò Àý´Ü ¹× BAP ó¸®ÇÑ pUC118Àº multi-cloning siteÀÇ ÇÑ °÷¸¸À» Àý´ÜÇÏ°í ºñ±³Àû »ç¿ëºóµµ°¡ ³ôÀº Á¦ÇÑÈ¿¼Ò¸¦ »ç¿ëÇÏ¿© pUC118À» Àý´ÜÇÑ ÈÄ ´ëÀå±Õ À¯·¡ÀÇ Alkaline Phosphatase (BAP)·Î Å»ÀλêÈÇÑ Å¬·Î´× º¤ÅÍÀÌ´Ù. º¤ÅÍ ¸¸ÀÇ self-ligationÀ» ¹æÁöÇÏ¿© ÇüÁúÀüȯ ¼¼Æ÷ÀÇ blank ¼öÄ¡¸¦ ³·Ãâ ¼ö ÀÖ´Ù.
pTV118NÀº ¿Ü·¡ À¯ÀüÀÚ¸¦ Á÷Á¢ ¹ßÇöÇÒ ¼ö ÀÖµµ·Ï pUC118¸¦ º¯Çü½ÃŲ ssDNA Á¦Á¶¿ëphagemid vectorÀÌ´Ù.
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promoter,
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ZÀÇ SD¼¿°ú ±× °³½Ã ÄÚµ·À» »ç¿ëÇÏ¿© ¿Ü·¡ À¯ÀüÀÚ¸¦ À¯µµ ¹ßÇöÇÒ ¼ö ÀÖ´Ù. SD¼¿°ú °³½Ã ÄÚµ·°£ÀÇ ¿°±â¼ö´Â ¹ø¿ª °³½Ã È¿À²ÀÌ ³ô´Ù°í º¸°íµÈ 8¿°±â·Î ¼³°èµÇ¾î ÀÖÀ¸¸ç, ¹ø¿ª frameÀ» ¸ÂÃß¾î ¿Ü·¡ À¯ÀüÀÚ¸¦ Ŭ·Î´×Çϱâ À§ÇÏ¿© 3 Á¾·ùÀÇ
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- ¿Ü·¡ À¯ÀüÀÚÀÇ cloning°ú
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promoter¸¦ ÀÌ¿ëÇÑ À¯ÀüÀÚ ¹ßÇö
- M13 primers¸¦ ÀÌ¿ëÇÑ DNA ¿°±â¼¿ °áÁ¤
- Kilo-Sequence¿ë Deletion Kit (Code 6030)¸¦ ÀÌ¿ëÇÑ ±ä ´ÜÆíÀÇ ¿°±â¼¿ °áÁ¤
»ç¿ë»óÀÇ ÁÖÀÇ
pTV118N˼
lac
Z ¿µ¿ª¿¡ ´ëÇÑ M13IG ¿µ¿ªÀÇ ¹æÇâÀÌ pUC118ÀÇ ¿ª¹æÇâÀ̹ǷΠM13 forward primer¸¦ »ç¿ëÇÏ¿© ÇÑ°¡´Ú DNAÀÇ ¿°±â¼¿À» °áÁ¤ÇÒ ¼ö ¾ø´Ù.
°ü·ÃÁ¦Ç°
M13 Primer RV-N (TaKaRa Code 3833)
M13 Primers (Code 3810, 3820, 3831, 3832A/B, 3830A/B)
pUC118/pUC119¿Í pTV118NÀÇ ±¸Á¶
Keyword :
Vector (Cloning ¿ë)
M13 mp18 Single Strand DNA£¨Virion DNA£©
pBR322 DNA
pKF18k-2/19k-3 DNA
pSTV28/29 DNA
pUC118/119 type vector
pUC18/19 type vector
È¿À² ³ôÀº TA-cloning¿¡
TaKaRa T-Vector pMD20